SR1 (SRI) (NM_003130) Human Tagged ORF Clone

CAT#: RG203130

SRI (GFP-tagged) - Human sorcin (SRI), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SRI
• Synonyms: CP-22; CP22; SCN; V19
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203130 representing NM_003130
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTACCCGGGGCATCCTGGCGCCGGCGGCGGGTACTACCCAGGCGGGTATGGAGGGGCTCCCGGAG
  GGCCTGCGTTTCCCGGACAAACTCAGGATCCGCTGTATGGTTACTTTGCTGCTGTAGCTGGACAGGATGG
  GCAGATAGATGCTGATGAATTGCAGAGATGTCTGACACAGTCTGGCATTGCTGGAGGATACAAACCTTTT
  AACCTGGAGACTTGCCGGCTTATGGTTTCAATGCTGGATAGAGATATGTCTGGCACAATGGGTTTCAATG
  AATTTAAAGAACTCTGGGCTGTACTGAATGGCTGGAGACAACACTTTATCAGTTTTGACACTGACAGGAG
  TGGAACAGTAGACCCACAAGAATTGCAGAAGGCCCTGACAACAATGGGATTTAGGTTGAGTCCCCAGGCT
  GTGAATTCAATTGCAAAACGATACAGCACCAATGGAAAGATCACCTTCGACGACTACATCGCCTGCTGCG
  TCAAACTGAGGGCTCTTACAGACAGCTTTCGAAGACGGGATACTGCTCAGCAAGGTGTTGTGAATTTCCC
  ATATGATGATTTCATTCAATGTGTCATGAGTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203130 representing NM_003130
  Red=Cloning site Green=Tags(s)
  
  MAYPGHPGAGGGYYPGGYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYKPF
  NLETCRLMVSMLDRDMSGTMGFNEFKELWAVLNGWRQHFISFDTDRSGTVDPQELQKALTTMGFRLSPQA
  VNSIAKRYSTNGKITFDDYIACCVKLRALTDSFRRRDTAQQGVVNFPYDDFIQCVMSV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003130
• ORF Size: 594 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003130.2, NP_003121.1
• RefSeq Size: 2030 bp
• RefSeq ORF: 597 bp
• Locus ID: 6717
• Cytogenetics: 7q21.12
• Domains: EFh
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]'