PI3 (NM_002638) Human Tagged ORF Clone
CAT#: RG203136
- TrueORF®
PI3 (GFP-tagged) - Human peptidase inhibitor 3, skin-derived (PI3)
"NM_002638" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PI3 |
Synonyms | cementoin; ESI; SKALP; WAP3; WFDC14 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203136 representing NM_002638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCCAGCAGCTTCTTGATCGTGGTGGTGTTCCTCATCGCTGGGACGCTGGTTCTAGAGGCAGCTG TCACGGGAGTTCCTGTTAAAGGTCAAGACACTGTCAAAGGCCGTGTTCCATTCAATGGACAAGATCCCGT TAAAGGACAAGTTTCAGTTAAAGGTCAAGATAAAGTCAAAGCGCAAGAGCCAGTCAAAGGTCCAGTCTCC ACTAAGCCTGGCTCCTGCCCCATTATCTTGATCCGGTGCGCCATGTTGAATCCCCCTAACCGCTGCTTGA AAGATACTGACTGCCCAGGAATCAAGAAGTGCTGTGAAGGCTCTTGCGGGATGGCCTGTTTCGTTCCCCA G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203136 representing NM_002638
Red=Cloning site Green=Tags(s) MRASSFLIVVVFLIAGTLVLEAAVTGVPVKGQDTVKGRVPFNGQDPVKGQVSVKGQDKVKAQEPVKGPVS TKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMACFVPQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002638 |
ORF Size | 351 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002638.2, NP_002629.1 |
RefSeq Size | 623 bp |
RefSeq ORF | 354 bp |
Locus ID | 5266 |
Cytogenetics | 20q13.12 |
Protein Families | Secreted Protein, Transmembrane |
Gene Summary | 'This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC122631 | PI3 (untagged)-Human peptidase inhibitor 3, skin-derived (PI3) |
USD 310.00 |
|
RC203136 | PI3 (Myc-DDK-tagged)-Human peptidase inhibitor 3, skin-derived (PI3) |
USD 98.00 |
|
RC203136L1 | Lenti ORF clone of Human peptidase inhibitor 3, skin-derived (PI3), Myc-DDK-tagged |
USD 768.00 |
|
RC203136L2 | Lenti ORF clone of Human peptidase inhibitor 3, skin-derived (PI3), mGFP tagged |
USD 620.00 |
|
RC203136L3 | Lenti ORF clone of Human peptidase inhibitor 3, skin-derived (PI3), Myc-DDK-tagged |
USD 768.00 |
|
RC203136L4 | Lenti ORF clone of Human peptidase inhibitor 3, skin-derived (PI3), mGFP tagged |
USD 768.00 |
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