NSDHL (NM_015922) Human Tagged ORF Clone

CAT#: RG203225

NSDHL (GFP-tagged) - Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NSDHL
• Synonyms: H105E3; SDR31E1; XAP104
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203225 representing NM_015922
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAGCAGTTAGCGAGCCAATGAGAGACCAAGTCGCACGGACTCATTTGACAGAGGACACTCCCA
  AAGTGAATGCTGACATAGAAAAGGTTAACCAGAATCAGGCCAAGAGATGCACAGTGATCGGTGGCTCTGG
  ATTCCTGGGGCAGCACATGGTGGAGCAGTTGCTGGCAAGAGGATATGCTGTCAATGTATTTGATATCCAG
  CAAGGGTTTGATAATCCCCAGGTGCGGTTCTTTCTGGGTGACCTCTGCAGCCGACAGGATCTGTACCCAG
  CTCTGAAAGGTGTAAACACAGTTTTCCACTGTGCGTCACCCCCACCATCCAGTAACAACAAGGAGCTCTT
  TTATAGAGTGAATTACATTGGCACCAAGAATGTCATTGAAACTTGCAAAGAGGCTGGGGTTCAGAAACTC
  ATTTTAACCAGCAGTGCCAGTGTCATCTTTGAGGGCGTCGATATCAAGAATGGAACTGAAGACCTTCCCT
  ATGCCATGAAACCCATTGACTACTACACAGAGACTAAGATCTTACAGGAGAGGGCAGTTCTGGGCGCCAA
  CGATCCTGAGAAGAATTTCTTAACCACAGCCATCCGCCCTCATGGCATTTTCGGCCCAAGGGACCCGCAG
  TTGGTACCCATCCTCATCGAGGCAGCCAGGAACGGCAAGATGAAGTTCGTGATTGGAAATGGGAAGAACT
  TGGTGGACTTCACCTTTGTGGAGAACGTGGTCCATGGACACATCCTGGCGGCAGAGCAGCTCTCCCGAGA
  CTCGACACTGGGTGGGAAGGCATTTCACATCACCAATGATGAGCCCATCCCTTTCTGGACATTCCTGTCT
  CGCATCCTGACAGGCCTCAATTATGAGGCCCCCAAGTACCACATCCCCTACTGGGTGGCCTACTACCTGG
  CCCTCCTGCTATCCCTGCTGGTGATGGTGATCAGTCCTGTCATCCAGCTGCAGCCCACCTTCACACCCAT
  GCGGGTCGCACTGGCTGGCACATTCCACTACTACAGCTGCGAGAGAGCCAAAAAGGCCATGGGCTACCAG
  CCACTAGTGACCATGGATGATGCTATGGAGAGGACCGTGCAGAGCTTTCGCCACCTGCGGAGGGTCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203225 representing NM_015922
  Red=Cloning site Green=Tags(s)
  
  MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQ
  QGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKL
  ILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQ
  LVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLS
  RILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQ
  PLVTMDDAMERTVQSFRHLRRVK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015922
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015922.2, NP_057006.1
• RefSeq Size: 1581
• RefSeq ORF: 1122
• Locus ID: 50814
• Domains: 3Beta_HSD
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]