ISYNA1 (NM_016368) Human Tagged ORF Clone

CAT#: RG203452

ISYNA1 (GFP-tagged) - Human inositol-3-phosphate synthase 1 (ISYNA1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ISYNA1
• Synonyms: INO1; INOS; IPS; IPS-1; IPS 1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203452 representing NM_016368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGCCGCCCAGTTCTTCGTCGAGAGCCCGGACGTGGTCTACGGCCCCGAGGCCATCGAGGCGC
  AATACGAGTACCGGACGACGCGCGTCAGCCGCGAGGGTGGCGTTCTCAAGGTGCACCCCACGTCCACGCG
  CTTCACCTTCCGGACCGCCCGGCAGGTGCCCCGGCTCGGGGTCATGCTTGTCGGCTGGGGCGGGAACAAC
  GGCTCCACACTCACCGCCGCGGTGCTGGCCAATCGACTGCGTTTGTCCTGGCCCACGCGCAGCGGCCGCA
  AGGAGGCCAACTACTACGGCTCGCTGACTCAGGCGGGCACCGTGAGCCTGGGCCTGGACGCCGAGGGCCA
  GGAGGTGTTCGTACCCTTCAGCGCGGTGCTGCCCATGGTGGCGCCCAACGACCTCGTGTTCGATGGCTGG
  GACATCTCGTCGCTGAACCTGGCCGAGGCGATGCGGCGCGCGAAGGTGCTGGACTGGGGGCTGCAGGAGC
  AACTGTGGCCGCACATGGAGGCCCTGCGGCCCCGGCCTTCTGTTTACATCCCCGAATTCATCGCGGCCAA
  CCAGAGCGCGCGCGCGGACAACCTCATCCCAGGCTCGCGTGCGCAGCAGCTGGAGCAGATCCGCAGGGAC
  ATCCGAGACTTCCGGTCTAGCGCGGGGCTGGACAAAGTCATAGTGCTGTGGACGGCGAACACGGAGCGCT
  TCTGTGAGGTGATTCCAGGCCTCAACGACACAGCCGAGAACCTGCTGCGCACCATTGAGCTCGGTCTGGA
  GGTGTCGCCCTCCACGCTCTTCGCCGTGGCCAGCATCCTGGAGGGCTGTGCCTTCCTCAATGGGTCTCCG
  CAGAACACCCTGGTGCCCGGAGCTCTTGAGCTCGCGTGGCAGCACCGGGTTTTTGTGGGCGGAGATGACT
  TCAAGTCAGGCCAGACCAAAGTCAAGTCCGTGCTTGTGGACTTCCTCATTGGCTCCGGCCTCAAGACCAT
  GTCCATCGTGAGTTACAACCACCTGGGCAACAACGATGGGGAGAACCTATCGGCGCCATTGCAGTTCCGC
  TCTAAGGAGGTGTCCAAGAGCAACGTGGTGGACGACATGGTGCAGAGCAACCCAGTGCTCTATACGCCCG
  GCGAAGAGCCTGACCACTGCGTGGTCATCAAGTATGTGCCGTACGTGGGTGACAGCAAGCGCGCGCTGGA
  TGAGTATACCTCGGAGCTGATGCTGGGCGGAACCAACACACTGGTGCTGCACAACACGTGTGAGGACTCG
  CTGCTGGCCGCACCCATCATGCTGGACCTAGCGCTGCTGACCGAGCTGTGCCAGCGCGTGAGCTTCTGCA
  CTGACATGGACCCCGAGCCGCAGACCTTCCACCCCGTGCTGTCCCTGCTCAGCTTCCTCTTCAAGGCGCC
  ACTAGTGCCGCCCGGCAGCCCGGTGGTCAATGCGCTTTTCCGCCAGCGCAGCTGCATCGAGAACATCCTC
  AGGGCCTGCGTGGGGCTCCCGCCACAGAACCACATGCTCCTGGAACACAAAATGGAGCGCCCAGGGCCCA
  GCCTCAAGCGAGTTGGACCCGTGGCTGCCACCTACCCTATGTTGAACAAGAAAGGACCGGTACCCGCTGC
  CACCAATGGCTGCACCGGTGATGCCAATGGGCATCTGCAAGAGGAGCCCCCAATGCCCACCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203452 representing NM_016368
  Red=Cloning site Green=Tags(s)
  
  MEAAAQFFVESPDVVYGPEAIEAQYEYRTTRVSREGGVLKVHPTSTRFTFRTARQVPRLGVMLVGWGGNN
  GSTLTAAVLANRLRLSWPTRSGRKEANYYGSLTQAGTVSLGLDAEGQEVFVPFSAVLPMVAPNDLVFDGW
  DISSLNLAEAMRRAKVLDWGLQEQLWPHMEALRPRPSVYIPEFIAANQSARADNLIPGSRAQQLEQIRRD
  IRDFRSSAGLDKVIVLWTANTERFCEVIPGLNDTAENLLRTIELGLEVSPSTLFAVASILEGCAFLNGSP
  QNTLVPGALELAWQHRVFVGGDDFKSGQTKVKSVLVDFLIGSGLKTMSIVSYNHLGNNDGENLSAPLQFR
  SKEVSKSNVVDDMVQSNPVLYTPGEEPDHCVVIKYVPYVGDSKRALDEYTSELMLGGTNTLVLHNTCEDS
  LLAAPIMLDLALLTELCQRVSFCTDMDPEPQTFHPVLSLLSFLFKAPLVPPGSPVVNALFRQRSCIENIL
  RACVGLPPQNHMLLEHKMERPGPSLKRVGPVAATYPMLNKKGPVPAATNGCTGDANGHLQEEPPMPTT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016368
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016368.3, NP_057452.1
• RefSeq Size: 1852
• RefSeq ORF: 1677
• Locus ID: 51477
• Domains: Inos-1-P_synth
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways
• Gene Summary: This gene encodes an inositol-3-phosphate synthase enzyme. The encoded protein plays a critical role in the myo-inositol biosynthesis pathway by catalyzing the rate-limiting conversion of glucose 6-phosphate to myoinositol 1-phosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011]