DACT3 (NM_145056) Human Tagged ORF Clone

CAT#: RG203525

DACT3 (GFP-tagged) - Human dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DACT3
• Synonyms: DAPPER3; RRR1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203525 representing NM_145056
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCAGCCCGCGGCCCTGCGGCCGCCCTCCCACCGACTCGCCCGACGCGGGGGGCGCAGGGCGGCCCC
  TGGACGGCTACATCTCGGCGCTCCTGCGCAGGCGCCGCCGCCGGGGGGCGGGCCAGCCCCGGACCAGTCC
  TGGGGGCGCGGACGGCGGCCCGCGGCGCCAGAACAGCGTGCGCCAGCGGCCGCCCGACGCGTCTCCGTCC
  CCCGGCAGCGCGCGACCCGCGCGGGAGCCCTCGTTGGAGCGCGTCGGGGGCCACCCCACCAGCCCTGCCG
  CCTTGAGCCGCGCCTGGGCGTCGTCGTGGGAGTCGGAGGCGGCACCCGAGCCCGCTGCGCCGCCCGCCGC
  CCCCTCACCCCCCGACAGCCCGGCTGAGGGCCGCTTGGTGAAGGCGCAGTACATCCCGGGCGCGCAGGCG
  GCCACCCGAGGCCTCCCTGGCCGCGCCGCCCGCCGCAAACCGCCGCCACTGACCCGCGGCCGCAGCGTGG
  AGCAGTCACCACCCCGGGAGCGTCCCCGGGCCGCCGGCCGCCGTGGACGCATGGCCGAGGCTTCGGGCCG
  CCGCGGCTCGCCCAGGGCCCGCAAGGCCTCGCGCTCCCAGTCTGAGACCAGCCTGCTGGGCCGCGCCTCC
  GCGGTCCCTTCGGGGCCCCCTAAGTACCCCACGGCGGAGCGGGAAGAGCCTCGGCCTCCACGGCCACGCC
  GCGGCCCAGCGCCCACGCTGGCGGCCCAGGCCGCAGGGTCCTGCCGTCGCTGGCGCTCCACTGCGGAGAT
  CGACGCTGCCGATGGGCGCCGCGTGCGGCCCCGAGCCCCTGCGGCGCGTGTTCCCGGCCCCGGCCCGTCC
  CCGTCAGCTCCCCAGCGTCGTCTGCTTTACGGCTGCGCGGGCAGCGACTCCGAGTGCTCGGCTGGGCGCC
  TGGGGCCCCTGGGACGCCGGGGGCCTGCGGGAGGCGTCGGCGGGGGTTACGGGGAGAGCGAATCGAGCGC
  CAGCGAGGGAGAATCGCCTGCCTTCAGCTCTGCCTCCAGCGACTCAGACGGCAGCGGTGGCCTCGTGTGG
  CCGCAGCAGCTGGTGGCGGCCACCGCGGCCTCTGGGGGTGGAGCAGGTGCAGGGGCGCCCGCAGGCCCCG
  CCAAAGTCTTCGTGAAAATCAAAGCTTCCCACGCGCTCAAGAAAAAGATACTGCGTTTCCGTTCGGGTTC
  TCTCAAGGTCATGACTACAGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203525 representing NM_145056
  Red=Cloning site Green=Tags(s)
  
  MRSPRPCGRPPTDSPDAGGAGRPLDGYISALLRRRRRRGAGQPRTSPGGADGGPRRQNSVRQRPPDASPS
  PGSARPAREPSLERVGGHPTSPAALSRAWASSWESEAAPEPAAPPAAPSPPDSPAEGRLVKAQYIPGAQA
  ATRGLPGRAARRKPPPLTRGRSVEQSPPRERPRAAGRRGRMAEASGRRGSPRARKASRSQSETSLLGRAS
  AVPSGPPKYPTAEREEPRPPRPRRGPAPTLAAQAAGSCRRWRSTAEIDAADGRRVRPRAPAARVPGPGPS
  PSAPQRRLLYGCAGSDSECSAGRLGPLGRRGPAGGVGGGYGESESSASEGESPAFSSASSDSDGSGGLVW
  PQQLVAATAASGGGAGAGAPAGPAKVFVKIKASHALKKKILRFRSGSLKVMTTV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145056
• ORF Size: 1212 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145056.1, NP_659493.1
• RefSeq Size: 2544
• RefSeq ORF: 1890
• Locus ID: 147906