KLHL9 (NM_018847) Human Tagged ORF Clone

CAT#: RG203728

KLHL9 (GFP-tagged) - Human kelch-like 9 (Drosophila) (KLHL9)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLHL9
• Synonyms: RP11-380P16.6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203728 representing NM_018847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGTCCCTTGGTAACGGCGAAATGGGCGTCTCTGCCCATTTGCAGCCTTGTAAGGCAGGAACCA
  CACGCTTTTTTACCAGCAATACTCACAGTTCGGTGGTATTGCAAGGCTTTGATCAGCTTAGAATAGAAGG
  ATTGCTTTGTGATGTGACCCTGGTACCAGGTGATGGAGATGAAATCTTCCCTGTTCACAGAGCTATGATG
  GCGTCTGCTAGTGATTATTTCAAAGCCATGTTCACAGGTGGAATGAAAGAACAAGATTTGATGTGCATTA
  AGCTTCATGGGGTGAACAAGGTTGGTCTGAAGAAAATCATTGATTTTATTTATACTGCAAAACTTTCTCT
  TAATATGGACAATCTTCAGGACACACTTGAAGCTGCTAGCTTTTTACAAATATTACCCGTTTTGGATTTC
  TGTAAAGTATTTCTTATATCAGGAGTCTCTTTGGATAATTGTGTTGAGGTTGGACGAATTGCTAACACCT
  ACAATCTTATAGAAGTGGATAAATATGTTAATAATTTCATCCTGAAGAACTTTCCTGCTTTATTGAGTAC
  TGGGGAGTTTCTAAAACTCCCTTTTGAACGACTTGCATTTGTGCTTTCCAGTAATAGTCTTAAGCACTGT
  ACCGAACTTGAACTCTTTAAGGCAGCCTGTCGCTGGCTAAGGTTGGAAGACCCTCGGATGGATTATGCTG
  CAAAGTTAATGAAGAATATTCGATTTCCACTGATGACACCACAGGATCTCATCAATTACGTGCAGACAGT
  AGATTTCATGAGAACAGACAATACCTGCGTGAATTTGCTTTTGGAAGCTAGCAATTACCAAATGATGCCA
  TATATGCAGCCAGTGATGCAGTCAGATAGAACTGCCATTCGATCTGACTCCACTCACTTGGTTACATTAG
  GAGGAGTTTTGAGGCAGCAGCTGGTTGTCAGTAAAGAATTACGGATGTATGATGAAAGGGCACAAGAATG
  GAGATCTTTAGCCCCAATGGATGCTCCCCGTTACCAGCATGGTATTGCTGTCATTGGAAACTTTCTTTAT
  GTAGTTGGTGGTCAGAGTAATTATGATACAAAAGGAAAAACTGCTGTTGATACAGTTTTCAGATTTGATC
  CTCGGTATAATAAATGGATGCAGGTTGCATCATTAAATGAAAAGCGCACATTCTTTCACTTGAGTGCCCT
  CAAAGGACATTTGTATGCAGTTGGTGGGCGCAGTGCAGCTGGTGAACTGGCCACAGTAGAATGTTACAAC
  CCAAGAATGAATGAGTGGAGCTATGTTGCAAAAATGAGTGAACCCCACTATGGTCATGCTGGAACAGTAT
  ATGGAGGCTTAATGTATATTTCAGGAGGAATTACCCATGACACTTTCCAAAATGAGCTCATGTGTTTTGA
  CCCAGATACAGATAAATGGATGCAAAAGGCTCCAATGACTACAGTCAGAGGTCTGCATTGCATGTGTACA
  GTTGGAGATAAGCTCTATGTCATTGGTGGCAATCACTTCAGAGGAACAAGTGATTATGATGATGTTCTAA
  GCTGTGAATACTATTCACCAACCCTTGACCAGTGGACCCCAATTGCCGCCATGTTAAGAGGCCAAAGTGA
  TGTTGGAGTTGCCGTCTTTGAAAATAAAATCTATGTTGTTGGTGGATATTCTTGGAATAATCGTTGTATG
  GTAGAAATTGTCCAGAAATATGACCCAGAAAAAGATGAGTGGCATAAAGTTTTTGATCTTCCAGAGTCAC
  TTGGTGGCATTCGAGCCTGTACACTCACAGTTTTTCCACCTGAAGAAAACCCTGGGTCACCTTCTAGAGA
  ATCACCTCTTTCAGCACCTTCAGATCATTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203728 representing NM_018847
  Red=Cloning site Green=Tags(s)
  
  MKVSLGNGEMGVSAHLQPCKAGTTRFFTSNTHSSVVLQGFDQLRIEGLLCDVTLVPGDGDEIFPVHRAMM
  ASASDYFKAMFTGGMKEQDLMCIKLHGVNKVGLKKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDF
  CKVFLISGVSLDNCVEVGRIANTYNLIEVDKYVNNFILKNFPALLSTGEFLKLPFERLAFVLSSNSLKHC
  TELELFKAACRWLRLEDPRMDYAAKLMKNIRFPLMTPQDLINYVQTVDFMRTDNTCVNLLLEASNYQMMP
  YMQPVMQSDRTAIRSDSTHLVTLGGVLRQQLVVSKELRMYDERAQEWRSLAPMDAPRYQHGIAVIGNFLY
  VVGGQSNYDTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHLSALKGHLYAVGGRSAAGELATVECYN
  PRMNEWSYVAKMSEPHYGHAGTVYGGLMYISGGITHDTFQNELMCFDPDTDKWMQKAPMTTVRGLHCMCT
  VGDKLYVIGGNHFRGTSDYDDVLSCEYYSPTLDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCM
  VEIVQKYDPEKDEWHKVFDLPESLGGIRACTLTVFPPEENPGSPSRESPLSAPSDHS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018847
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018847.2, NP_061335.1
• RefSeq Size: 4412
• RefSeq ORF: 1854
• Locus ID: 55958
• Domains: BTB, Kelch
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a protein that belongs to the kelch repeat-containing family, and contains an N-terminal BTB/POZ domain, a BACK domain and six C-terminal kelch repeats. The encoded protein is a component of a complex with cullin 3-based E3 ligase, which plays a role in mitosis. This protein complex is a cell cycle regulator, and functions in the organization and integrity of the spindle midzone in anaphase and the completion of cytokinesis. The complex is required for the removal of the chromosomal passenger protein aurora B from mitotic chromosomes. [provided by RefSeq, Jul 2016]