MAT1A (NM_000429) Human Tagged ORF Clone
CAT#: RG203765
- TrueORF®
MAT1A (GFP-tagged) - Human methionine adenosyltransferase I, alpha (MAT1A)
"NM_000429" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | MAT1A |
Synonyms | MAT; MATA1; SAMS; SAMS1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203765 representing NM_000429
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGACCGGTGGATGGCTTGTGTGACCACTCTCTAAGTGAAGGAGTCTTCATGTTCACATCGGAGT CTGTGGGAGAGGGACACCCGGATAAGATCTGTGACCAGATCAGTGATGCAGTGCTGGATGCCCATCTCAA GCAAGACCCCAATGCCAAGGTGGCCTGTGAGACAGTGTGCAAGACCGGCATGGTGCTGCTGTGTGGTGAG ATCACCTCAATGGCCATGGTGGACTACCAGCGGGTGGTGAGGGACACCATCAAGCACATCGGCTACGATG ACTCAGCCAAGGGCTTTGACTTCAAGACTTGCAACGTGCTGGTGGCTTTGGAGCAGCAATCCCCAGATAT TGCCCAGTGCGTCCATCTGGACAGAAATGAGGAGGATGTGGGGGCAGGAGATCAGGGTTTGATGTTCGGC TATGCCACCGACGAGACAGAGGAGTGCATGCCCCTCACCATCATCCTTGCTCACAAGCTCAACGCCCGGA TGGCAGACCTCAGGCGCTCCGGCCTCCTCCCCTGGCTGCGGCCTGACTCTAAGACTCAGGTGACAGTTCA GTACATGCAGGACAATGGCGCAGTCATCCCTGTGCGCATCCACACCATCGTCATCTCTGTGCAGCACAAC GAAGACATCACGCTGGAGGAGATGCGCAGGGCCCTGAAGGAGCAAGTCATCAGGGCCGTGGTGCCGGCCA AGTACCTGGACGAAGACACCGTCTACCACCTGCAGCCCAGTGGGCGGTTTGTCATCGGAGGTCCCCAGGG GGATGCGGGTGTCACTGGCCGTAAGATTATTGTGGACACCTATGGCGGCTGGGGGGCTCATGGTGGTGGG GCCTTCTCTGGGAAGGACTACACCAAGGTGGACCGCTCAGCCGCTTATGCTGCCCGCTGGGTGGCCAAGT CTCTGGTGAAAGCAGGGCTCTGCCGGAGAGTGCTTGTCCAGGTTTCCTATGCCATTGGTGTGGCCGAGCC GCTGTCCATTTCCATCTTCACCTACGGAACCTCTCAGAAGACAGAGCGAGAGCTGCTGGATGTGGTGCAT AAGAACTTCGACCTCCGGCCGGGCGTCATTGTCAGGGATTTGGACTTGAAGAAGCCCATCTACCAGAAGA CAGCATGCTACGGCCATTTCGGAAGAAGCGAGTTCCCATGGGAGGTTCCCAGGAAGCTTGTATTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203765 representing NM_000429
Red=Cloning site Green=Tags(s) MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGE ITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFG YATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHN EDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGG AFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVH KNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000429 |
ORF Size | 1185 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000429.2, NP_000420.1 |
RefSeq Size | 3419 bp |
RefSeq ORF | 1188 bp |
Locus ID | 4143 |
Cytogenetics | 10q22.3 |
Domains | S-AdoMet_synt |
Protein Families | Druggable Genome |
Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism |
Gene Summary | 'This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119881 | MAT1A (untagged)-Human methionine adenosyltransferase I, alpha (MAT1A) |
USD 310.00 |
|
RC203765 | MAT1A (Myc-DDK-tagged)-Human methionine adenosyltransferase I, alpha (MAT1A) |
USD 420.00 |
|
RC203765L3 | Lenti ORF clone of Human methionine adenosyltransferase I, alpha (MAT1A), Myc-DDK-tagged |
USD 620.00 |
|
RC203765L4 | Lenti ORF clone of Human methionine adenosyltransferase I, alpha (MAT1A), mGFP tagged |
USD 620.00 |
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