MAT1A (NM_000429) Human Untagged Clone

CAT#: SC119881

MAT1A (untagged)-Human methionine adenosyltransferase I, alpha (MAT1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAT1A
• Synonyms: MAT; MATA1; SAMS; SAMS1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119881 sequence for NM_000429 edited (data generated by NextGen Sequencing)
  ATGAATGGACCGGTGGATGGCTTGTGTGACCACTCTCTAAGTGAAGGAGTCTTCATGTTC
  ACATCGGAGTCTGTGGGAGAGGGACACCCGGATAAGATCTGTGACCAGATCAGTGATGCA
  GTGCTGGATGCCCATCTCAAGCAAGACCCCAATGCCAAGGTGGCCTGTGAGACAGTGTGC
  AAGACCGGCATGGTGCTGCTGTGTGGTGAGATCACCTCAATGGCCATGGTGGACTACCAG
  CGGGTGGTGAGGGACACCATCAAGCACATCGGCTACGATGACTCAGCCAAGGGCTTTGAC
  TTCAAGACTTGCAACGTGCTGGTGGCTTTGGAGCAGCAATCCCCAGATATTGCCCAGTGC
  GTCCATCTGGACAGAAATGAGGAGGATGTGGGGGCAGGAGATCAGGGTTTGATGTTCGGC
  TATGCCACCGACGAGACAGAGGAGTGCATGCCCCTCACCATCATCCTTGCTCACAAGCTC
  AACGCCCGGATGGCAGACCTCAGGCGCTCCGGCCTCCTCCCCTGGCTGCGGCCTGACTCT
  AAGACTCAGGTGACAGTTCAGTACATGCAGGACAATGGCGCAGTCATCCCTGTGCGCATC
  CACACCATCGTCATCTCTGTGCAGCACAACGAAGACATCACGCTGGAGGAGATGCGCAGG
  GCCCTGAAGGAGCAAGTCATCAGGGCCGTGGTGCCGGCCAAGTACCTGGACGAAGACACC
  GTCTACCACCTGCAGCCCAGTGGGCGGTTTGTCATCGGAGGTCCCCAGGGGGATGCGGGT
  GTCACTGGCCGTAAGATTATTGTGGACACCTATGGCGGCTGGGGGGCTCATGGTGGTGGG
  GCCTTCTCTGGGAAGGACTACACCAAGGTGGACCGCTCAGCCGCATATGCTGCCCGCTGG
  GTGGCCAAGTCTCTGGTGAAAGCAGGGCTCTGCCGGAGAGTGCTTGTCCAGGTTTCCTAT
  GCCATTGGTGTGGCCGAGCCGCTGTCCATTTCCATCTTCACCTACGGAACCTCTCAGAAG
  ACAGAGCGAGAGCTGCTGGATGTGGTGCATAAGAACTTCGACCTCCGGCCGGGCGTCATT
  GTCAGGGATTTGGACTTGAAGAAGCCCATCTACCAGAAGACAGCATGCTACGGCCATTTC
  GGAAGAAGCGAGTTCCCATGGGAGGTTCCCAGGAAGCTTGTATTTTAG
  
  Clone variation with respect to NM_000429.2
  426 t=>c;870 a=>g;882 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000429 unedited
  NGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGCTCAAAATGTTCACTGGAA
  AAAAGCGTGAGTGGAGAAGTGTGAGAAGATGAATGGACCGGTGGATGGCTTGTGTGACCA
  CTCTCTAAGTGAAGGAGTCTTCATGTTCACATCGGAGTCTGTGGGAGAGGGACACCCGGA
  TAAGATCTGTGACCAGATCAGTGATGCAGTGCTGGATGCCCATCTCAAGCAAGACCCCAA
  TGCCAAGGTGGCCTGTGAGACAGTGTGCAAGACCGGCATGGTGCTGCTGTGTGGTGAGAT
  CACCTCAATGGCCATGGTGGACTACCAGCGGGTGGTGAGGGACACCATCAAGCACATCGG
  CTACGATGACTCAGCCAAGGGCTTTGACTTCAAGACTTGCAACGTGCTGGTGGCTTTGGA
  GCAGCAATCCCCAGATATTGCCCAGTGCGTCCATCTGGACAGAAATGAGGAGGATGTGGG
  GGCAGGAGATCAGGGTTTGATGTTCGGCTATGCCACCGACGAGACAGAGGAGTGCATGCC
  CCTCACCATCATCCTTGCTCACAAGCTCAACGCCCGGATGGCAGACCTCANGCGCTTCGG
  CCTCCTNCCCTGGCTGCGGCCTGACTCTAAGACTCANGTGACAGTTCAGTACATGCAGGA
  CATGGGCGCAGTCATCCCTGTGCGCATNCACACATCGTCATCTCTGTGCAGCACAACGAA
  GACATCACGCTGGNAGAGATGCGCANGGCCCCTGAGGAGCAGTCATCGGGCCCGTGGNTG
  CCGNCCAGTACCTGNACGAAGACACCGTCTACCCCTGCAGCCAGTGGNGCGGTTNGTCAT
  CGGAGGTCCCAGGGGATGGCGGNTGTCACTGCCGTAGAATATTGTGGACACTATGGCGGC
  TGGGGCTATTGGGGGGGTGGGGCTCTNCTGGAAGGACTACACAAGTGGACCGTCACCGCT
  ATCTGCCCCTGGTGGCAGTCTTGNGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000429 unedited
  NAAGGTTTTTACTNTGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTT
  TTTAGTTGCTCCTTGAGTTTTTATTCATTGTTTTAGCAGGAACTATTAACAAATAAAGGC
  TTTAGTTTCATACAAACAAACTGACAAAAAAGATCTTATTTCTCTAGAGGGTAGGGAAAG
  TATAAAATTCTGAATTTTCATGTCGAGTGTGAGCCAAGTTAGAGGAACTTGGCCACCTGC
  AAACCACTCCCTCCCTCCATGGGAAGGAATCTGAGGCTTCCTAGGTGACCAGGAGCCGGG
  CTTCTTTTGTTGCCTTAATTTCCTTTCACCTGAGAGAAAATGAAAGCCAGGGTCTTTGTG
  CTCCAGGCCAAGGCCCAGATGCTGGCTGCCTGCTCCCCAGATGAGACTTCCTGGGCTGCC
  TCTCCCAGCACCATGTACCCTAGAGAGAGGCCAAGTCCCCAGTGTTCCCCTGGGGCCCAT
  GGAGGCCCCTGCCTGAGCCTGTGGTCCTGGGAGCTGGGGAGAGGTANAGATGCTGCTGTC
  AGAAGTCCAGGCTAAGGACAAGTGGGGCANGTANGGCANGTGCTGCTGGCCATGGATGGA
  AAGGCANNGCAGTTTCCAGACCTTAGCANGCCAAGGACTGCTCANGCCCCTTGGGAGCAG
  ACCCGCTCCCAGACAGTACCTGGNCAAAGCAGAATCTNCTCATGACGTCTCAAAGCCTGT
  CCGACACCAGAAGGCAGCCCCGCCTATTTACATGAGGCCCATAGACCAGCCTNCCCTGAT
  CCCGAAAGGGCACTCACTTCCTTCAATGCCTCAGNCTGCAGGGGATACAGGGACCANNGA
  AAGAGAAAGACTGAGGCCTCTGTCTAGGGNGCTGCCTNTGTGCTGGAACAAGTGCTGCTC
  TGCTCCAGAGCCAGATAGACTGGACTGACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000429
• Insert Size: 3520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000429.2, NP_000420.1
• RefSeq Size: 3419 bp
• RefSeq ORF: 1188 bp
• Locus ID: 4143
• Cytogenetics: 10q22.3
• Domains: S-AdoMet_synt
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: 'This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008]'