PACS1 (NM_018026) Human Tagged ORF Clone

CAT#: RG204036

PACS1 (GFP-tagged) - Human phosphofurin acidic cluster sorting protein 1 (PACS1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PACS1
• Synonyms: MRD17; SHMS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204036 representing NM_018026
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAACGCGGAGGGGCGGGCGGTGGTCCCGGAGGCGCCGGGGGCGGCAGCGGCCAGCGGGGATCCG
  GGGTCGCCCAGTCCCCTCAGCAGCCGCCGCCGCAGCAGCAGCAGCAGCAGCCGCCGCAGCAGCCGACGCC
  CCCCAAGCTGGCCCAGGCCACCTCGTCGTCCTCGTCCACCTCGGCGGCGGCTGCCTCCTCCTCGTCCTCG
  TCTACCTCCACCTCCATGGCCGTGGCGGTGGCCTCGGGCTCCGCGCCTCCCGGTGGCCCGGGGCCAGGCC
  GCACCCCCGCCCCGGTGCAGATGAACCTGTACGCCACCTGGGAGGTGGACCGGAGCTCGTCCAGCTGCGT
  GCCTAGGCTATTCAGCTTGACCCTGAAGAAACTCGTCATGCTAAAAGAAATGGACAAAGATCTTAACTCA
  GTGGTCATCGCTGTGAAGCTGCAGGGTTCAAAAAGAATTCTTCGCTCCAACGAGATCGTCCTTCCAGCTA
  GTGGACTGGTGGAAACAGAGCTCCAATTAACCTTCTCCCTTCAGTACCCTCATTTCCTTAAGCGAGATGC
  CAACAAGCTGCAGATCATGCTGCAAAGGAGAAAACGTTACAAGAATCGGACCATCTTGGGCTATAAGACC
  TTGGCCGTGGGACTCATCAACATGGCAGAGGTGATGCAGCATCCTAATGAAGGCGCACTGGTGCTTGGCC
  TACACAGCAACGTGAAGGATGTCTCTGTGCCTGTGGCAGAAATAAAGATCTACTCCCTGTCCAGCCAACC
  CATTGACCATGAAGGAATCAAATCCAAGCTTTCTGATCGTTCTCCTGATATTGACAATTATTCTGAGGAA
  GAGGAAGAGAGTTTCTCATCAGAACAGGAAGGCAGTGATGATCCATTGCATGGGCAGGACTTGTTCTACG
  AAGACGAAGATCTCCGGAAAGTGAAGAAGACCCGGAGGAAACTAACCTCAACCTCTGCCATCACAAGGCA
  ACCTAACATCAAACAGAAGTTTGTGGCCCTCCTGAAGCGGTTTAAAGTTTCAGATGAGGTGGGCTTTGGG
  CTGGAGCATGTGTCCCGCGAGCAGATCCGGGAAGTGGAAGAGGACTTGGATGAATTGTATGACAGTCTGG
  AGATGTACAACCCCAGCGACAGTGGCCCTGAGATGGAGGAGACAGAAAGCATCCTCAGCACGCCAAAGCC
  CAAGCTCAAGCCTTTCTTTGAGGGGATGTCGCAGTCCAGCTCCCAGACGGAGATTGGCAGCCTCAACAGC
  AAAGGCAGCCTCGGAAAAGACACCACCAGCCCTATGGAATTGGCTGCTCTAGAAAAAATTAAATCTACTT
  GGATTAAAAACCAAGATGACAGCTTGACTGAAACAGACACTCTGGAAATCACTGACCAGGACATGTTTGG
  AGATGCCAGCACGAGTCTGGTTGTGCCGGAGAAAGTCAAAACTCCCATGAAGTCCAGTAAAACGGATCTC
  CAGGGCTCTGCCTCCCCCAGCAAAGTGGAGGGGGTGCACACACCCCGGCAGAAGAGGAGCACGCCCCTGA
  AGGAGCGGCAGCTCTCCAAGCCCCTAAGTGAGAGGACCAACAGTTCCGACAGCGAGCGCTCCCCAGATCT
  GGGCCACAGCACGCAGATTCCAAGAAAGGTGGTGTATGACCAGCTCAATCAGATCCTGGTGTCAGATGCA
  GCCCTCCCAGAAAATGTCATTCTGGTGAACACCACTGACTGGCAGGGCCAGTATGTGGCTGAGCTGCTCC
  AGGACCAGCGGAAGCCTGTGGTGTGCACCTGCTCCACCGTGGAGGTCCAGGCCGTGCTGTCCGCCCTGCT
  CACCCGGATCCAGCGCTACTGCAACTGCAACTCTTCCATGCCGAGGCCAGTGAAGGTGGCTGCTGTGGGA
  GGCCAGAGCTACCTGAGCTCCATCCTCAGGTTCTTTGTCAAGTCCCTGGCCAACAAGACCTCCGACTGGC
  TTGGCTACATGCGCTTCCTCATCATCCCCCTCGGTTCTCACCCTGTGGCCAAATACTTGGGGTCAGTCGA
  CAGTAAATACAGTAGTTCCTTCCTGGATTCTGGTTGGAGAGATCTGTTCAGTCGCTCGGAGCCACCAGTG
  TCAGAGCAACTGGACGTGGCAGGGCGGGTGATGCAGTACGTCAACGGGGCAGCCACGACACACCAGCTTC
  CCGTGGCCGAAGCCATGCTGACTTGCCGGCATAAGTTCCCTGATGAAGACTCCTATCAGAAGTTTATTCC
  CTTCATTGGCGTGGTGAAGGTGGGTCTGGTTGAAGACTCTCCCTCCACAGCAGGCGATGGGGACGATTCT
  CCTGTGGTCAGCCTTACTGTGCCCTCCACATCACCACCCTCCAGCTCGGGCCTGAGCCGAGACGCCACGG
  CCACCCCTCCCTCCTCCCCATCTATGAGCAGCGCCCTGGCCATCGTGGGGAGCCCTAATAGCCCATATGG
  GGACGTGATTGGCCTCCAGGTGGACTACTGGCTGGGCCACCCCGGGGAGCGGAGGAGGGAAGGCGACAAG
  AGGGACGCCAGCTCGAAGAACACCCTCAAGAGTGTCTTCCGCTCAGTGCAGGTGTCCCGCCTGCCCCATA
  GTGGGGAGGCCCAGCTTTCTGGCACCATGGCCATGACTGTGGTCACCAAAGAAAAGAACAAGAAAGTTCC
  CACCATCTTCCTGAGCAAGAAACCCCGAGAAAAGGAGGTGGATTCTAAGAGCCAGGTCATTGAAGGCATC
  AGCCGCCTCATCTGCTCAGCCAAGCAGCAGCAGACTATGCTGAGAGTGTCCATCGATGGGGTCGAGTGGA
  GTGACATCAAGTTCTTCCAGCTGGCAGCCCAGTGGCCCACCCATGTCAAGCACTTTCCAGTGGGACTCTT
  CAGTGGCAGCAAGGCCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204036 representing NM_018026
  Red=Cloning site Green=Tags(s)
  
  MAERGGAGGGPGGAGGGSGQRGSGVAQSPQQPPPQQQQQQPPQQPTPPKLAQATSSSSSTSAAAASSSSS
  STSTSMAVAVASGSAPPGGPGPGRTPAPVQMNLYATWEVDRSSSSCVPRLFSLTLKKLVMLKEMDKDLNS
  VVIAVKLQGSKRILRSNEIVLPASGLVETELQLTFSLQYPHFLKRDANKLQIMLQRRKRYKNRTILGYKT
  LAVGLINMAEVMQHPNEGALVLGLHSNVKDVSVPVAEIKIYSLSSQPIDHEGIKSKLSDRSPDIDNYSEE
  EEESFSSEQEGSDDPLHGQDLFYEDEDLRKVKKTRRKLTSTSAITRQPNIKQKFVALLKRFKVSDEVGFG
  LEHVSREQIREVEEDLDELYDSLEMYNPSDSGPEMEETESILSTPKPKLKPFFEGMSQSSSQTEIGSLNS
  KGSLGKDTTSPMELAALEKIKSTWIKNQDDSLTETDTLEITDQDMFGDASTSLVVPEKVKTPMKSSKTDL
  QGSASPSKVEGVHTPRQKRSTPLKERQLSKPLSERTNSSDSERSPDLGHSTQIPRKVVYDQLNQILVSDA
  ALPENVILVNTTDWQGQYVAELLQDQRKPVVCTCSTVEVQAVLSALLTRIQRYCNCNSSMPRPVKVAAVG
  GQSYLSSILRFFVKSLANKTSDWLGYMRFLIIPLGSHPVAKYLGSVDSKYSSSFLDSGWRDLFSRSEPPV
  SEQLDVAGRVMQYVNGAATTHQLPVAEAMLTCRHKFPDEDSYQKFIPFIGVVKVGLVEDSPSTAGDGDDS
  PVVSLTVPSTSPPSSSGLSRDATATPPSSPSMSSALAIVGSPNSPYGDVIGLQVDYWLGHPGERRREGDK
  RDASSKNTLKSVFRSVQVSRLPHSGEAQLSGTMAMTVVTKEKNKKVPTIFLSKKPREKEVDSKSQVIEGI
  SRLICSAKQQQTMLRVSIDGVEWSDIKFFQLAAQWPTHVKHFPVGLFSGSKAT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018026
• ORF Size: 2889 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018026.3, NP_060496.2
• RefSeq Size: 4511
• RefSeq ORF: 2892
• Locus ID: 55690
• Gene Summary: This gene encodes a protein with a putative role in the localization of trans-Golgi network (TGN) membrane proteins. Mouse and rat homologs have been identified and studies of the homologous rat protein indicate a role in directing TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. In addition, the human protein plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance. [provided by RefSeq, Jul 2008]