ALG14 (NM_144988) Human Tagged ORF Clone

CAT#: RG204062

ALG14 (GFP-tagged) - Human asparagine-linked glycosylation 14 homolog (S. cerevisiae) (ALG14)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALG14
• Synonyms: CMS15
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204062 representing NM_144988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTGCGTTCTCGTTCTAGCTGCGGCCGCAGGAGCTGTGGCGGTTTTCCTAATCCTGCGAATATGGG
  TAGTGCTTCGTTCCATGGACGTTACGCCCCGGGAGTCTCTCAGTATCTTGGTAGTGGCTGGGTCCGGTGG
  GCATACCACTGAGATCCTGAGGCTGCTTGGGAGCTTGTCCAATGCCTACTCACCTAGACATTATGTCATT
  GCTGACACTGATGAAATGAGTGCCAATAAAATAAATTCTTTTGAACTAGATCGAGCTGATAGAGACCCTA
  GTAACATGTATACCAAATACTACATTCACCGAATTCCAAGAAGCCGGGAGGTTCAGCAGTCCTGGCCCTC
  CACCGTTTTCACCACCTTGCACTCCATGTGGCTCTCCTTTCCCCTAATTCACAGGGTGAAGCCAGATTTG
  GTGTTGTGTAACGGACCAGGAACATGTGTTCCTATCTGTGTATCTGCCCTTCTCCTTGGGATACTAGGAA
  TAAAGAAAGTGATCATTGTCTACGTTGAAAGCATCTGCCGTGTAGAAACGTTATCCATGTCCGGAAAGAT
  TCTGTTTCATCTCTCAGATTACTTCATTGTTCAGTGGCCGGCTCTGAAAGAAAAGTATCCCAAATCGGTG
  TACCTTGGGCGAATTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204062 representing NM_144988
  Red=Cloning site Green=Tags(s)
  
  MVCVLVLAAAAGAVAVFLILRIWVVLRSMDVTPRESLSILVVAGSGGHTTEILRLLGSLSNAYSPRHYVI
  ADTDEMSANKINSFELDRADRDPSNMYTKYYIHRIPRSREVQQSWPSTVFTTLHSMWLSFPLIHRVKPDL
  VLCNGPGTCVPICVSALLLGILGIKKVIIVYVESICRVETLSMSGKILFHLSDYFIVQWPALKEKYPKSV
  YLGRIV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144988
• ORF Size: 648 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_144988.2, NP_659425.1
• RefSeq Size: 1032
• RefSeq ORF: 651
• Locus ID: 199857
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene is a member of the glycosyltransferase 1 family. The encoded protein and ALG13 are thought to be subunits of UDP-GlcNAc transferase, which catalyzes the first two committed steps in endoplasmic reticulum N-linked glycosylation. Mutations in this gene have been linked to congenital myasthenic syndrome (CMSWTA). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]