ACADSB (NM_001609) Human Tagged ORF Clone

CAT#: RG204465

ACADSB (GFP-tagged) - Human acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACADSB
• Synonyms: 2-MEBCAD; ACAD7; SBCAD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204465 representing NM_001609
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCCTGGCAGTGCGGTTGCTGCGCGGCAGCAAGCTGCTAAGAAGAAATTTCCTGACTTGTTTGT
  CTTCTTGGAAGATTCCTCCTCATGTCTCAAAATCTTCCCAGTCAGAAGCTCTACTCAATATAACAAATAA
  TGGAATACACTTTGCTCCCCTGCAAACATTTACAGATGAGGAAATGATGATAAAGAGTTCAGTTAAAAAA
  TTTGCTCAGGAACAAATTGCACCTTTGGTTTCAACCATGGATGAAAATTCGAAAATGGAGAAATCAGTAA
  TACAAGGATTATTTCAACAAGGGTTGATGGGTATTGAAGTTGACCCAGAATATGGAGGCACAGGAGCTTC
  ATTTTTATCCACTGTGCTCGTGATAGAGGAATTAGCCAAAGTTGATGCATCTGTGGCTGTCTTTTGTGAG
  ATCCAGAACACATTAATTAACACACTGATTAGAAAACATGGAACAGAAGAACAAAAGGCCACCTATTTGC
  CTCAGCTCACTACAGAAAAAGTAGGAAGTTTCTGCCTTTCAGAGGCTGGAGCAGGTAGTGACTCATTTGC
  TTTGAAGACCAGAGCTGATAAAGAGGGAGATTATTATGTCCTCAATGGATCAAAGATGTGGATCAGCAGT
  GCTGAGCACGCAGGGCTCTTTCTGGTGATGGCAAATGTAGACCCTACCATTGGATATAAGGGAATTACCT
  CCTTCTTAGTAGATCGTGATACTCCGGGCCTTCATATAGGGAAACCTGAAAACAAATTGGGGCTCAGAGC
  TTCTTCCACCTGCCCGTTAACATTCGAAAATGTCAAGGTTCCAGAAGCCAATATCTTGGGACAAATTGGA
  CATGGCTATAAGTATGCCATAGGGAGTCTCAATGAAGGTAGAATAGGAATTGCTGCACAGATGCTGGGAC
  TGGCGCAAGGATGTTTTGACTACACTATTCCATATATTAAAGAAAGGATACAATTTGGCAAAAGACTATT
  TGATTTTCAGGGCCTCCAACACCAAGTGGCTCACGTGGCCACCCAGCTGGAAGCTGCAAGATTACTAACA
  TACAATGCTGCTAGGCTTTTAGAAGCTGGAAAGCCATTCATAAAAGAAGCGTCAATGGCCAAATACTATG
  CATCAGAGATTGCAGGACAAACAACGAGTAAATGTATCGAGTGGATGGGGGGAGTAGGCTACACCAAAGA
  TTACCCTGTGGAGAAATACTTCCGAGATGCAAAGATTGGTACGATATATGAAGGAGCTTCCAACATCCAG
  TTGAACACCATTGCAAAGCATATCGATGCAGAATAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204465 representing NM_001609
  Red=Cloning site Green=Tags(s)
  
  MEGLAVRLLRGSKLLRRNFLTCLSSWKIPPHVSKSSQSEALLNITNNGIHFAPLQTFTDEEMMIKSSVKK
  FAQEQIAPLVSTMDENSKMEKSVIQGLFQQGLMGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCE
  IQNTLINTLIRKHGTEEQKATYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISS
  AEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANILGQIG
  HGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLT
  YNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQ
  LNTIAKHIDAEY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001609
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001609.3, NP_001600.1
• RefSeq Size: 5941
• RefSeq ORF: 1299
• Locus ID: 36
• Domains: Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
• Protein Pathways: Fatty acid metabolism, Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul 2008]