ARHGEF1 (NM_004706) Human Tagged ORF Clone

CAT#: RG204530

ARHGEF1 (GFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARHGEF1
• Synonyms: GEF1; IMD62; LBCL2; LSC; P115-RHOGEF; SUB1.5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204530 representing NM_004706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTCTTTCCACCTGGAGCAGCCCTGCAGAGCCCAGGGAGATGGAAGACTTCGCCCGAGGGGCGG
  CCTCCCCAGGCCCCTCCCGGCCTGGCCTGGTTCCCGTCAGCATCATCGGGGCTGAGGATGAGGATTTTGA
  GAACGAGCTGGAGACAAACTCAGAAGAGCAAAACAGCCAGTTCCAGAGCCTGGAGCAGGTGAAGCGGCGC
  CCAGCCCACCTCATGGCCCTCCTGCAGCACGTGGCCCTGCAGTTTGAGCCAGGACCCCTGCTTTGCTGTC
  TGCATGCCGACATGCTGGGCTCACTGGGCCCCAAGGAGGCCAAGAAGGCCTTCCTGGACTTCTACCACAG
  CTTCCTGGAGAAGACAGCGGTTCTCCGGGTGCCGGTCCCTCCCAACGTCGCCTTTGAACTTGACCGCACT
  AGGGCTGACCTCATCTCCGAGGATGTCCAGCGGCGGTTCGTGCAGGAGGTGGTGCAAAGCCAGCAGGTAG
  CCGTGGGCCGGCAGCTGGAGGACTTCCGTTCCAAGCGGCTCATGGGCATGACGCCCTGGGAGCAGGAGCT
  GGCCCAGCTGGAGGCTTGGGTTGGGCGGGACCGAGCCAGCTACGAGGCCCGGGAGCGGCACGTGGCGGAG
  CGGCTGCTCATGCACCTGGAGGAGATGCAACATACCATCTCTACCGACGAAGAAAAGAGTGCTGCCGTGG
  TCAACGCCATTGGCCTGTACATGCGCCACCTTGGGGTGCGGACCAAGAGTGGAGACAAGAAGTCGGGGAG
  GAACTTCTTCCGGAAAAAGGTGATGGGGAACCGGCGGTCGGACGAGCCTGCCAAGACCAAGAAGGGGCTG
  AGCAGCATCCTGGATGCCGCCCGCTGGAACCGGGGAGAGCCCCAGGTTCCAGATTTTCGACACCTCAAAG
  CAGAGGTTGATGCCGAGAAGCCAGGTGCTACAGACCGGAAGGGAGGCGTGGGGATGCCCTCTCGGGACCG
  GAATATCGGGGCTCCTGGGCAGGACACCCCTGGAGTCTCTCTGCACCCTCTGTCCCTGGACAGCCCAGAC
  CGGGAACCAGGTGCTGACGCCCCCCTGGAGCTGGGGGACTCATCCCCGCAGGGCCCAATGAGCCTGGAGT
  CCTTGGCGCCCCCAGAGAGTACCGACGAGGGGGCCGAAACCGAGAGCCCCGAGCCTGGAGATGAGGGGGA
  GCCGGGGCGGTCGGGACTGGAGCTTGAACCAGAAGAGCCTCCCGGCTGGCGGGAACTCGTCCCCCCAGAC
  ACCCTGCACAGCCTGCCCAAGAGCCAGGTGAAGCGGCAGGAGGTCATCAGCGAGCTGCTGGTGACAGAGG
  CGGCCCACGTGCGCATGCTGCGGGTGCTGCACGACCTCTTCTTCCAGCCCATGGCAGAATGCCTGTTCTT
  CCCCTTGGAGGAGCTGCAGAACATCTTCCCCAGCCTGGACGAGCTCATCGAGGTGCATTCCCTGTTCCTC
  GATCGCCTGATGAAGCGGAGGCAGGAGAGTGGCTACCTCATCGAGGAGATCGGAGACGTGCTGCTGGCCC
  GGTTTGATGGTGCTGAGGGCTCCTGGTTCCAGAAAATCTCCTCCCGCTTCTGCAGCCGCCAGTCATTTGC
  CTTAGAGCAGCTCAAAGCCAAGCAACGCAAGGACCCTCGGTTCTGTGCCTTCGTGCAGGAAGCTGAGAGC
  CGCCCGCGGTGCCGCCGCCTGCAGCTGAAGGACATGATCCCCACGGAGATGCAGCGGCTGACCAAGTACC
  CCCTGCTCCTGCAGAGCATCGGGCAGAACACAGAAGAGCCCACAGAACGGGAGAAAGTGGAGCTGGCAGC
  CGAGTGCTGCCGGGAAATTCTACACCACGTCAACCAAGCCGTGCGTGACATGGAGGACCTGCTGAGGCTC
  AAGGACTATCAGCGGCGCCTGGACTTGTCCCACCTTCGGCAGAGCAGCGACCCTATGCTGAGCGAGTTCA
  AGAACCTGGACATCACCAAGAAGAAATTGGTCCACGAGGGCCCACTGACGTGGCGGGTGACTAAGGACAA
  GGCAGTGGAGGTGCATGTGCTGCTGCTGGACGACCTGCTGCTGCTGCTCCAGCGCCAGGACGAGCGGCTG
  CTGCTCAAGTCCCATAGCCGGACACTGACGCCCACGCCCGATGGCAAGACCATGCTGCGGCCCGTGCTGC
  GGCTCACCTCCGCCATGACCCGCGAGGTGGCCACCGATCACAAAGCCTTCTACGTCCTTTTTACCTGGGA
  CCAGGAGGCCCAGATATACGAGCTGGTGGCACAGACTGTGTCGGAGCGGAAAAACTGGTGTGCTCTCATC
  ACTGAGACTGCCGGATCCCTGAAAGTCCCTGCCCCTGCCTCTCGCCCTAAGCCCCGGCCCAGCCCGAGCA
  GCACCCGAGAACCCCTCCTCAGCAGCTCTGAGAACGGCAATGGTGGCCGAGAGACGTCTCCAGCTGATGC
  CCGGACCGAGAGAATCCTCAGTGACCTCCTGCCCTTCTGCAGACCAGGCCCCGAGGGCCAGCTCGCTGCC
  ACGGCCCTTCGGAAAGTGCTGTCCCTGAAGCAGCTTCTGTTTCCGGCGGAGGAAGACAATGGGGCGGGGC
  CTCCTCGAGATGGGGATGGGGTCCCAGGGGGCGGCCCCCTGAGCCCAGCACGGACCCAGGAAATCCAGGA
  GAACCTGCTCAGCTTGGAGGAGACCATGAAGCAGCTGGAGGAGTTGGAGGAGGAATTTTGCCGCCTGAGA
  CCCCTCCTGTCTCAGCTTGGGGGGAACTCTGTCCCCCAGCCTGGCTGCACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204530 representing NM_004706
  Red=Cloning site Green=Tags(s)
  
  MASLSTWSSPAEPREMEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRR
  PAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRT
  RADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAE
  RLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGL
  SSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPD
  REPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPD
  TLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFL
  DRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAES
  RPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRL
  KDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERL
  LLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALI
  TETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAA
  TALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLR
  PLLSQLGGNSVPQPGCT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004706
• ORF Size: 2784 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004706.3, NP_004697.2
• RefSeq Size: 3236
• RefSeq ORF: 2739
• Locus ID: 9138
• Domains: RhoGEF, PH
• Protein Pathways: Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]