ARHGEF1 (NM_004706) Human Untagged Clone

CAT#: SC117214

ARHGEF1 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGEF1
• Synonyms: GEF1; IMD62; LBCL2; LSC; P115-RHOGEF; SUB1.5
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117214 sequence for NM_004706 edited (data generated by NextGen Sequencing)
  ATGGAAGACTTCGCCCGAGGGGCGGCCTCCCCAGGCCCCTCCCGGCCTGGCCTGGTTCCC
  GTCAGCATCATCGGGGCTGAGGATGAGGATTTTGAGAACGAGCTGGAGACAAACTCAGAA
  GAGCAAAACAGCCAGTTCCAGAGCCTGGAGCAGGTGAAGCGGCGCCCAGCCCACCTCATG
  GCCCTCCTGCAGCACGTGGCCCTGCAGTTTGAGCCAGGACCCCTGCTTTGCTGTCTGCAT
  GCCGACATGCTGGGCTCACTGGGCCCCAAGGAGGCCAAGAAGGCCTTCCTGGACTTCTAC
  CACAGCTTCCTGGAGAAGACAGCGGTTCTCCGGGTGCCGGTCCCTCCCAACGTCGCCTTT
  GAACTTGACCGCACTAGGGCTGACCTCATCTCCGAGGATGTCCAGCGGCGGTTCGTGCAG
  GAGGTGGTGCAAAGCCAGCAGGTAGCCGTGGGCCGGCAGCTGGAGGACTTCCGTTCCAAG
  CGGCTCATGGGCATGACGCCCTGGGAGCAGGAGCTGGCCCAGCTGGAGGCTTGGGTTGGG
  CGGGACCGAGCCAGCTACGAGGCCCGGGAGCGGCACGTGGCGGAGCGGCTGCTCATGCAC
  CTGGAGGAGATGCAACATACCATCTCTACCGACGAAGAAAAGAGTGCTGCCGTGGTCAAC
  GCCATTGGCCTGTACATGCGCCACCTTGGGGTGCGGACCAAGAGTGGAGACAAGAAGTCG
  GGGAGGAACTTCTTCCGGAAAAAGGTGATGGGGAACCGGCGGTCGGACGAGCCTGCCAAG
  ACCAAGAAGGGGCTGAGCAGCATCCTGGATGCCGCCCGCTGGAACCGGGGAGAGCCCCAG
  GTTCCAGATTTTCGACACCTCAAAGCAGAGGTTGATGCCGAGAAGCCAGGTGCTACAGAC
  CGGAAGGGAGGCGTGGGGATGCCCTCTCGGGACCGGAATATCGGGGCTCCTGGGCAGGAC
  ACCCCTGGAGTCTCTCTGCACCCTCTGTCCCTGGACAGCCCAGACCGGGAACCAGGTGCT
  GACGCCCCCCTGGAGCTGGGGGACTCATCCCCGCAGGGCCCAATGAGCCTGGAGTCCTTG
  GCGCCCCCAGAGAGTACCGACGAGGGGGCCGAAACCGAGAGCCCCGAGCCTGGAGATGAG
  GGGGAGCCGGGGCGGTCGGGACTGGAGCTTGAACCAGAAGAGCCTCCCGGCTGGCGGGAA
  CTCGTCCCCCCAGACACCCTGCACAGCCTGCCCAAGAGCCAGGTGAAGCGGCAGGAGGTC
  ATCAGCGAGCTGCTGGTGACAGAGGCGGCCCACGTGCGCATGCTGCGGGTGCTGCACGAC
  CTCTTCTTCCAGCCCATGGCAGAATGCCTGTTCTTCCCCTTGGAGGAGCTGCAGAACATC
  TTCCCCAGCCTGGACGAGCTCATCGAGGTGCATTCCCTGTTCCTCGATCGCCTGATGAAG
  CGGAGGCAGGAGAGTGGCTACCTCATCGAGGAGATCGGAGACGTGCTGCTGGCCCGGTTT
  GATGGTGCTGAGGGCTCCTGGTTCCAGAAAATCTCCTCCCGCTTCTGCAGCCGCCAGTCA
  TTTGCCTTAGAGCAGCTCAAAGCCAAGCAACGCAAGGACCCTCGGTTCTGTGCCTTCGTG
  CAGGAAGCTGAGAGCCGCCCGCGGTGCCGCCGCCTGCAGCTGAAGGACATGATCCCCACG
  GAGATGCAGCGGCTGACCAAGTACCCCCTGCTCCTGCAGAGCATCGGGCAGAACACAGAA
  GAGCCCACAGAACGGGAGAAAGTGGAGCTGGCAGCCGAGTGCTGCCGGGAAATTCTACAC
  CACGTCAACCAAGCCGTGCGTGACATGGAGGACCTGCTGAGGCTCAAGGACTATCAGCGG
  CGCCTGGACTTGTCCCACCTTCGGCAGAGCAGCGACCCTATGCTGAGCGAGTTCAAGAAC
  CTGGACATCACCAAGAAGAAATTGGTCCACGAGGGCCCACTGACGTGGCGGGTGACTAAG
  GACAAGGCAGTGGAGGTGCATGTGCTGCTGCTGGACGACCTGCTGCTGCTGCTCCAGCGC
  CAGGACGAGCGGCTGCTGCTCAAGTCCCATAGCCGGACACTGACGCCCACGCCCGATGGC
  AAGACCATGCTGCGGCCCGTGCTGCGGCTCACCTCCGCCATGACCCGCGAGGTGGCCACC
  GATCACAAAGCCTTCTACGTCCTTTTTACCTGGGACCAGGAGGCCCAGATATACGAGCTG
  GTGGCACAGACTGTGTCGGAGCGGAAAAACTGGTGTGCTCTCATCACTGAGACTGCCGGA
  TCCCTGAAAGTCCCTGCCCCTGCCTCTCGCCCTAAGCCCCGGCCCAGCCCGAGCAGCACC
  CGAGAACCCCTCCTCAGCAGCTCTGAGAACGGCAATGGTGGCCGAGAGACGTCTCCAGCT
  GATGCCCGGACCGAGAGAATCCTCAGTGACCTCCTGCCCTTCTGCAGACCAGGCCCCGAG
  GGCCAGCTCGCTGCCACGGCCCTTCGGAAAGTGCTGTCCCTGAAGCAGCTTCTGTTTCCG
  GCGGAGGAAGACAATGGGGCGGGGCCTCCTCGAGATGGGGATGGGGTCCCAGGGGGCGGC
  CCCCTGAGCCCAGCACGGACCCAGGAAATCCAGGAGAACCTGCTCAGCTTGGAGGAGACC
  ATGAAGCAGCTGGAGGAGTTGGAGGAGGAATTTTGCCGCCTGAGACCCCTCCTGTCTCAG
  CTTGGGGGGAACTCTGTCCCCCAGCCTGGCTGCACTTGA
  
  Clone variation with respect to NM_004706.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004706 unedited
  GCACGAGGCGGGATCGCCGAGCCCGACCTCGGGCGCCCCGCCGGTCACCTCCGCGCGGAC
  ACCAGCCCTGCAGAGCCCAGGGAGATGGAAGACTTCGCCCGAGGGGCGGCCTCCCCAGGC
  CCCTCCCGGCCTGGCCTGGTTCCCGTCAGCATCATCGGGGCTGAGGATGAGGATTTTGAG
  AACGAGCTGGAGACAAACTCAGAAGAGCAAAACAGCCAGTTCCAGAGCCTGGAGCAGGTG
  AAGCGGCGCCCAGCCCACCTCATGGCCCTCCTGCAGCACGTGGCCCTGCAGTTTGAGCCA
  GGACCCCTGCTTTGCTGTCTGCATGCCGACATGCTGGGCTCACTGGGCCCCAAGGAGGCC
  AAGAAGGCCTTCCTGGACTTCTACCACAGCTTCCTGGAGAAGACAGCGGTTCTCCGGGTG
  CCGGTCCCTCCCAACGTCGCCTTTGAACTTGACCGCACTAGGGCTGACCTCATCTCCGAG
  GATGTCCAGCGGCGGTTCGTGCAGGAGGTGGTGCAAAGCCAGCAGGTAGCCGTGGGCCGG
  CAGCTGGAGGACTTCCGTTCCAAGCGGCTCATGGGCATGACGCCCTGGGAGCAGGAGCTG
  GCCCAGCTGGAGGCTTGNGTTGGGCGGGACCGAGCCAGCTACGAGGCCCGGGAGCGGCAC
  GTGGCGGAGCGGCTGCTCATGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004706 unedited
  GGGACTATGTACCTCGGCCGCACCTCNAAAGTCGAGTTTTTTTTTTTTTTTTTTCTTCTT
  AGATAATATATATTAAAAAATAAACCTCCAATTCAGGCTATAAAAACAGATCGAATGCAC
  TTGGGGGTGGGGGTGGAGGGGTGNCCCCCACACGCCATCCTGANATGGCCCGGGTCACCG
  TGGTGCCCTCCANAATGGAGCCCTGAGTCCCCCAATCTTTTTTTTTGAGGGAGACATGGC
  CTTGGCTCGTGCGGGGCCTCTAACCCCATCTGGTCCCCTCCCATGCGTGGCCCACATCTC
  CCTCTCCTCATGCCCTCGGGGTGTGAGATGCTGCCGCAGCTGTGTGGGTGGGGGTGGTCC
  CTTACCTCCTCTCCCCCNTTCCTCTCCTTCTTGCNTAATGCCTTCCTGGGCGGGCACCCT
  CTCTCCCCCTCGTCTTTGTGTCACATTTCCCCCCCATCTTTTCCCTGCCCGTTCTCAGGC
  CGCAATTTTCCCCCTCCCTCTCCTCCCCCTCCTTTATGTCCCCCTCCAACCTGCCCANGC
  TCTCCTTGCCCCTCCTCCCCCCCCCCCCTCTTCTTGGCCCCCCCCCTTTTTCCCCTCTCT
  CTCTTTGCCTTTCCCCCCCCCCTTTTTTTCCTCTCCCCTATCCCACCCTCTTTTTTCTCC
  CCTCTTTCCCCATGCTTCTTTTCTCTTCCTTCCCCTTTTGCCTTCTCTCCCCCTTTTTCT
  TTTGCCCCCCCCGCCTCTTCTTCCCCCCCACCTTCCTTTCCCGTTCCTTTTCTTTCCCTT
  CCTTTCCCTTTCCTCCCTCCTCTCCCTTTGTCCCTTTTCCCTTCTTCTCCTTCCCCCCCC
  CCCCGTCCTCGCCCTCTCTCTCCTTCCCCTTNACCTTCCTCGTTCCCCTCTTTTCACTTC
  ACTCAACCACCCCTTAAT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004706
• ORF Size: 2739 bp
• Insert Size: 3400
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_004706.3, NP_004697.2
• RefSeq Size: 3236
• RefSeq ORF: 2739
• Locus ID: 9138
• Domains: RhoGEF, PH
• Protein Pathways: Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream start codon, as compared to variant 1. The encoded isoform 2 thus has a shorter N-terminus, as compared to isoform 1.