PSG9 (NM_002784) Human Tagged ORF Clone

CAT#: RG204742

PSG9 (GFP-tagged) - Human pregnancy specific beta-1-glycoprotein 9 (PSG9)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PSG9
• Synonyms: PS34; PSBG-9; PSBG-11; PSG11; PSGII
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204742 representing NM_002784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCCTCCCAGCCCCTTCCTGCACACAGCGCATCACCTGGAAGGGGCTCCTGCTCACAGCATCAC
  TTTTAAACTTCTGGAACCCGCCCACCACTGCCGAAGTCACGATTGAAGCCCAGCCACCCAAAGTTTCTGA
  GGGGAAGGATGTTCTTCTACTTGTCCACAATTTGCCCCAGAATCTTCCTGGCTACTTCTGGTACAAAGGG
  GAAATGACGGACCTCTACCATTACATTATATCGTATATAGTTGATGGTAAAATAATTATATATGGGCCTG
  CATACAGTGGAAGAGAAACAGTATATTCCAACGCATCCCTGCTGATCCAGAATGTCACCCGGAAGGATGC
  AGGAACCTACACCTTACACATCATAAAGCGAGGTGATGAGACTAGAGAAGAAATTCGACATTTCACCTTC
  ACCTTATACTTGGAGACTCCCAAGCCCTACATCTCCAGCAGCAACTTAAACCCCAGGGAGGCCATGGAGG
  CTGTGCGCTTAATCTGTGATCCTGAGACTCTGGACGCAAGCTACCTATGGTGGATGAATGGTCAGAGCCT
  CCCTGTGACTCACAGGTTGCAGCTGTCCAAAACCAACAGGACCCTCTATCTATTTGGTGTCACAAAGTAT
  ATTGCAGGACCCTATGAATGTGAAATACGGAACCCAGTGAGTGCCAGTCGCAGTGACCCAGTCACCCTGA
  ATCTCCTCCCGAAGCTGCCCATCCCCTACATCACCATCAACAACTTAAACCCCAGGGAGAATAAGGATGT
  CTTAGCCTTCACCTGTGAACCTAAGAGTGAGAACTACACCTACATTTGGTGGCTAAACGGTCAGAGCCTC
  CCCGTCAGTCCCGGGGTAAAGCGACCCATTGAAAACAGGATACTCATTCTACCCAGTGTCACGAGAAATG
  AAACAGGACCCTATCAATGTGAAATACAGGACCGATATGGTGGCCTCCGCAGTAACCCAGTCATCCTAAA
  TGTCCTCTATGGTCCAGACCTCCCCAGAATTTACCCTTCATTCACCTATTACCGTTCAGGAGAAAACCTC
  GACTTGTCCTGCTTCACGGAATCTAACCCACCGGCAGAGTATTTTTGGACAATTAATGGGAAGTTTCAGC
  AATCAGGACAAAAGCTCTTTATCCCCCAAATTACTAGAAATCATAGCGGGCTCTATGCTTGCTCTGTTCA
  TAACTCAGCCACTGGCAAGGAAATCTCCAAATCCATGACAGTCAAAGTCTCTGGTCCCTGCCATGGAGAC
  CTGACAGAGTCTCAGTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204742 representing NM_002784
  Red=Cloning site Green=Tags(s)
  
  MGPLPAPSCTQRITWKGLLLTASLLNFWNPPTTAEVTIEAQPPKVSEGKDVLLLVHNLPQNLPGYFWYKG
  EMTDLYHYIISYIVDGKIIIYGPAYSGRETVYSNASLLIQNVTRKDAGTYTLHIIKRGDETREEIRHFTF
  TLYLETPKPYISSSNLNPREAMEAVRLICDPETLDASYLWWMNGQSLPVTHRLQLSKTNRTLYLFGVTKY
  IAGPYECEIRNPVSASRSDPVTLNLLPKLPIPYITINNLNPRENKDVLAFTCEPKSENYTYIWWLNGQSL
  PVSPGVKRPIENRILILPSVTRNETGPYQCEIQDRYGGLRSNPVILNVLYGPDLPRIYPSFTYYRSGENL
  DLSCFTESNPPAEYFWTINGKFQQSGQKLFIPQITRNHSGLYACSVHNSATGKEISKSMTVKVSGPCHGD
  LTESQS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002784
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002784.2, NP_002775.2
• RefSeq Size: 1731 bp
• RefSeq ORF: 1281 bp
• Locus ID: 5678
• Cytogenetics: 19q13.31
• Domains: ig, IGc2, IG
• Protein Families: Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a member of the pregnancy-specific glycoprotein (PSG) family. This protein family and the closely related carcinoembryonic antigen cell adhesion molecule (CEACAM) gene family are both members of the immunoglobulin superfamily, and are organized as a large gene cluster. This protein is thought to inhibit platelet-fibrinogen interactions. Several studies suggest that reduced serum concentrations of PSGs are associated with fetal growth restrictions, while up-regulation of this gene has been observed in colorectal cancers. Several pseudogenes of this gene are found on chromosome 19. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Sep 2014]'