HSPC014 (POMP) (NM_015932) Human Tagged ORF Clone

CAT#: RG204812

POMP (GFP-tagged) - Human proteasome maturation protein (POMP)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POMP
• Synonyms: C13orf12; HSPC014; PNAS-110; PRAAS2; UMP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204812 representing NM_015932
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCCAGAGGACTTGGATCTGAGCTAAAGGACAGTATTCCAGTTACTGAACTTTCAGCAAGTGGAC
  CTTTTGAAAGTCATGATCTTCTTCGGAAAGGTTTTTCTTGTGTGAAAAATGAACTTTTGCCTAGTCATCC
  CCTTGAATTATCAGAAAAAAATTTCCAGCTCAACCAAGATAAAATGAATTTTTCCACACTGAGAAACATT
  CAGGGTCTATTTGCTCCGCTAAAATTACAGATGGAATTCAAGGCAGTGCAGCAGGTTCAGCGTCTTCCAT
  TTCTTTCAAGCTCAAATCTTTCACTGGATGTTTTGAGGGGTAATGATGAGACTATTGGATTTGAGGATAT
  TCTTAATGATCCATCACAAAGCGAAGTCATGGGAGAGCCACACTTGATGGTGGAATATAAACTTGGTTTA
  CTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204812 representing NM_015932
  Red=Cloning site Green=Tags(s)
  
  MNARGLGSELKDSIPVTELSASGPFESHDLLRKGFSCVKNELLPSHPLELSEKNFQLNQDKMNFSTLRNI
  QGLFAPLKLQMEFKAVQQVQRLPFLSSSNLSLDVLRGNDETIGFEDILNDPSQSEVMGEPHLMVEYKLGL
  L
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015932
• ORF Size: 423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015932.2, NP_057016.1
• RefSeq Size: 1352
• RefSeq ORF: 426
• Locus ID: 51371
• Protein Pathways: Proteasome
• Gene Summary: The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5' UTR of this gene has been associated with KLICK syndrome, a rare skin disorder. [provided by RefSeq, Aug 2010]