ADO (NM_032804) Human Tagged ORF Clone

CAT#: RG205583

ADO (GFP-tagged) - Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADO
• Synonyms: C10orf22
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205583 representing NM_032804
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGAGACAACATGGCCTCCTTGATCCAACGGATCGCCCGCCAGGCTTGCCTCACCTTCCGGGGCA
  GCTGGGGCGGCCGCGGCGCTTCCGATCGCGACGCGGCTTCTGGCGCGGAGGCGCCGATGCAGCCGGGCTT
  CCCCGAGAACCTGAGCAAGCTGAAGAGCCTCCTGACCCAGCTCCGCGCCGAGGACTTGAACATCGCCCCG
  CGCAAGGCCACACTGCAGCCGCTGCCGCCCAACCTGCCGCCAGTCACCTACATGCACATCTACGAGACGG
  ACGGCTTCAGCCTGGGCGTGTTCCTGCTCAAGAGCGGCACGTCCATCCCGCTGCACGACCACCCGGGCAT
  GCACGGCATGCTCAAGGTGCTGTACGGCACCGTGCGCATCAGCTGCATGGACAAGCTAGACGCGGGCGGC
  GGGCAACGGCCGCGGGCCTTGCCGCCCGAGCAGCAGTTCGAGCCGCCGCTGCAGCCCCGGGAGCGAGAAG
  CCGTGCGGCCGGGCGTGCTGCGTTCGCGGGCCGAGTACACCGAGGCCAGCGGCCCCTGCATCCTCACACC
  GCACCGGGACAACCTGCACCAGATCGACGCCGTGGAAGGGCCTGCCGCCTTCCTGGACATCCTGGCCCCG
  CCCTACGACCCGGACGATGGCCGGGACTGCCACTATTACCGGGTGCTGGAGCCGGTCAGGCCCAAGGAGG
  CCTCCAGCTCGGCCTGTGACCTGCCTCGAGAGGTGTGGCTCCTGGAGACCCCACAGGCCGATGACTTCTG
  GTGCGAGGGAGAACCCTATCCAGGTCCCAAGGTCTTCCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205583 representing NM_032804
  Red=Cloning site Green=Tags(s)
  
  MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAP
  RKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGG
  GQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAP
  PYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032804
• ORF Size: 810 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032804.5, NP_116193.2
• RefSeq Size: 3739
• RefSeq ORF: 813
• Locus ID: 84890
• Protein Pathways: Metabolic pathways, Taurine and hypotaurine metabolism
• Gene Summary: Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine (Dominy et al., 2007 [PubMed 17581819]). [supplied by OMIM, Mar 2008]