DCLRE1B (NM_022836) Human Tagged ORF Clone

CAT#: RG205744

DCLRE1B (GFP-tagged) - Human DNA cross-link repair 1B (DCLRE1B)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DCLRE1B
• Synonyms: APOLLO; SNM1B; SNMIB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205744 representing NM_022836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGGGTCCTGATCCCCCATACGCCCATCGCAGTGGACTTCTGGAGCCTGCGCCGGGCTGGCACCG
  CACGTCTCTTCTTCTTGTCTCACATGCACTCGGACCACACCGTGGGCCTGTCTAGCACCTGGGCCCGGCC
  CCTCTACTGCTCCCCAATTACAGCCCACCTCTTGCATCGTCACCTACAGGTATCTAAGCAATGGATCCAA
  GCCCTGGAGGTTGGTGAGAGCCATGTATTACCCCTAGATGAAATTGGACAAGAGACCATGACCGTAACCC
  TCCTCGATGCCAATCACTGTCCTGGTTCTGTCATGTTTCTCTTTGAAGGATATTTTGGAACCATCCTCTA
  CACAGGTGATTTTCGATACACACCATCCATGCTAAAGGAGCCAGCCCTGACACTGGGGAAACAGATCCAT
  ACTTTATACCTAGACAACACCAATTGCAATCCAGCCCTGGTTCTTCCTTCCCGACAAGAAGCTGCCCACC
  AGATTGTCCAGCTCATTCGAAAACACCCACAACATAACATAAAGATTGGACTCTACAGCCTGGGAAAGGA
  ATCACTGCTGGAGCAGCTGGCCCTGGAGTTTCAGACCTGGGTGGTATTGAGTCCTCGGCGCCTGGAGTTG
  GTACAGCTACTGGGCCTGGCAGATGTGTTCACAGTGGAGGAGAAGGCTGGCCGCATCCATGCAGTAGACC
  ATATGGAGATCTGCCATTCCAACATGCTGCGTTGGAACCAGACCCACCCTACGATTGCTATCCTTCCCAC
  AAGCCGAAAAATCCACAGCTCCCACCCTGATATCCACGTCATCCCTTACTCTGACCATTCCTCTTACTCC
  GAGCTTCGTGCCTTTGTCGCAGCACTGAAGCCTTGCCAGGTGGTGCCCATTGTAAGTCGGCGGCCCTGTG
  GAGGCTTTCAGGACAGTCTGAGCCCCAGGATCTCCGTGCCCCTGATTCCGGACTCTGTACAGCAATACAT
  GAGTTCTTCCTCTAGAAAACCAAGCCTTCTCTGGCTGTTAGAAAGGAGGCTAAAGAGGCCGAGAACCCAA
  GGTGTTGTGTTTGAATCCCCTGAGGAAAGTGCTGATCAATCTCAAGCTGACAGAGACTCAAAGAAGGCCA
  AGAAAGAGAAACTTTCTCCCTGGCCTGCGGACCTTGAAAAGCAGCCTTCCCACCATCCTTTGCGGATCAA
  GAAGCAGTTGTTCCCAGATCTCTATAGCAAAGAATGGAACAAGGCAGTGCCTTTCTGTGAGTCTCAAAAG
  AGGGTGACTATGTTGACGGCCCCACTGGGATTTTCAGTGCACTTAAGGTCTACAGATGAGGAGTTTATTT
  CTCAAAAAACCAGGGAGGAAATTGGTTTAGGGTCCCCCTTGGTACCCATGGGAGATGATGATGGAGGTCC
  AGAAGCCACAGGGAATCAGAGTGCCTGGATGGGCCATGGTTCTCCCCTGTCCCACAGCAGCAAGGGCACC
  CCTCTTCTAGCTACTGAATTCAGGGGTCTAGCACTCAAATATCTTCTGACTCCAGTGAACTTTTTCCAGG
  CAGGGTATTCTTCCAGGAGATTTGACCAGCAAGTGGAAAAATACCATAAACCCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205744 representing NM_022836
  Red=Cloning site Green=Tags(s)
  
  MNGVLIPHTPIAVDFWSLRRAGTARLFFLSHMHSDHTVGLSSTWARPLYCSPITAHLLHRHLQVSKQWIQ
  ALEVGESHVLPLDEIGQETMTVTLLDANHCPGSVMFLFEGYFGTILYTGDFRYTPSMLKEPALTLGKQIH
  TLYLDNTNCNPALVLPSRQEAAHQIVQLIRKHPQHNIKIGLYSLGKESLLEQLALEFQTWVVLSPRRLEL
  VQLLGLADVFTVEEKAGRIHAVDHMEICHSNMLRWNQTHPTIAILPTSRKIHSSHPDIHVIPYSDHSSYS
  ELRAFVAALKPCQVVPIVSRRPCGGFQDSLSPRISVPLIPDSVQQYMSSSSRKPSLLWLLERRLKRPRTQ
  GVVFESPEESADQSQADRDSKKAKKEKLSPWPADLEKQPSHHPLRIKKQLFPDLYSKEWNKAVPFCESQK
  RVTMLTAPLGFSVHLRSTDEEFISQKTREEIGLGSPLVPMGDDDGGPEATGNQSAWMGHGSPLSHSSKGT
  PLLATEFRGLALKYLLTPVNFFQAGYSSRRFDQQVEKYHKPC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022836
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022836.2, NP_073747.1
• RefSeq Size: 3664
• RefSeq ORF: 1599
• Locus ID: 64858
• Protein Families: Druggable Genome
• Gene Summary: DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]). [supplied by OMIM, Mar 2008]