CPSF73 (CPSF3) (NM_016207) Human Tagged ORF Clone

CAT#: RG205834

CPSF3 (GFP-tagged) - Human cleavage and polyadenylation specific factor 3, 73kDa (CPSF3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CPSF3
• Synonyms: CPSF-73; CPSF73
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205834 representing NM_016207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCGATTCCTGCTGAGGAGAGCGACCAGCTGCTGATCCGACCCCTTGGAGCTGGGCAAGAAGTAG
  GAAGATCATGTATTATTCTCGAGTTCAAAGGAAGAAAAATAATGCTCGACTGTGGGATCCACCCTGGCCT
  AGAAGGAATGGATGCTCTTCCTTATATTGATTTAATTGACCCAGCTGAGATTGATCTCCTATTAATTAGT
  CATTTCCATTTGGATCACTGTGGAGCTCTGCCCTGGTTTCTACAGAAGACAAGTTTCAAAGGAAGAACAT
  TTATGACTCATGCCACAAAAGCTATTTATAGATGGCTTCTTTCTGATTATGTCAAAGTTAGTAACATATC
  AGCAGACGACATGCTGTATACCGAGACAGATTTGGAAGAAAGCATGGACAAAATTGAAACTATCAACTTT
  CATGAAGTTAAGGAAGTTGCGGGAATCAAGTTTTGGTGTTACCATGCAGGTCACGTCCTAGGAGCCGCCA
  TGTTCATGATTGAGATCGCAGGCGTGAAGCTTTTGTACACTGGTGATTTCTCAAGACAAGAAGATAGGCA
  CTTAATGGCAGCTGAAATTCCTAATATTAAGCCTGATATTCTTATCATTGAATCTACTTATGGGACCCAT
  ATCCATGAGAAACGTGAAGAGCGAGAAGCAAGATTCTGTAACACTGTCCACGATATTGTAAACAGAGGAG
  GCAGGGGTCTCATTCCTGTCTTTGCTCTTGGAAGGGCTCAGGAGCTGCTCTTGATTCTAGATGAGTACTG
  GCAGAATCACCCAGAACTACATGACATTCCAATATACTATGCATCATCTTTGGCCAAGAAGTGTATGGCA
  GTGTACCAGACATATGTAAATGCCATGAATGACAAAATCCGCAAACAGATCAACATCAATAATCCCTTTG
  TTTTCAAACACATTAGTAACCTCAAGAGCATGGATCATTTTGATGACATTGGTCCCAGTGTTGTAATGGC
  CTCCCCAGGCATGATGCAAAGTGGCTTATCCAGAGAATTATTTGAAAGCTGGTGTACTGATAAGAGGAAT
  GGTGTCATTATAGCGGGATACTGTGTAGAAGGGACACTTGCCAAGCACATCATGTCTGAACCTGAAGAAA
  TCACTACTATGTCTGGACAGAAGTTACCACTGAAAATGTCTGTTGATTACATTTCTTTCTCAGCTCACAC
  GGATTACCAGCAAACCAGTGAATTTATTCGTGCTTTGAAACCGCCTCATGTGATTTTAGTCCATGGAGAA
  CAGAATGAAATGGCCAGATTGAAAGCAGCACTGATTCGAGAATATGAAGATAACGATGAAGTTCACATAG
  AGGTTCATAATCCTCGGAATACAGAAGCAGTGACCTTAAACTTCAGAGGAGAAAAACTAGCCAAGGTTAT
  GGGATTTTTAGCAGACAAAAAACCAGAACAAGGCCAGCGGGTCTCAGGAATACTTGTTAAAAGAAACTTT
  AATTATCACATACTTTCTCCTTGCGACCTGTCCAATTATACTGACCTGGCCATGAGCACGGTGAAGCAGA
  CCCAAGCCATTCCATATACTGGTCCCTTTAATTTGCTCTGTTACCAGCTGCAGAAATTGACAGGTGATGT
  GGAAGAATTAGAAATTCAAGAAAAACCTGCTCTGAAAGTGTTCAAAAATATTACTGTAATACAAGAACCA
  GGCATGGTGGTATTAGAATGGCTGGCAAACCCTTCTAATGATATGTATGCAGATACAGTAACAACTGTGA
  TATTGGAAGTTCAGTCAAATCCCAAAATAAGAAAAGGTGCAGTACAGAAGGTTTCTAAAAAATTAGAAAT
  GCACGTTTACAGCAAGAGGTTGGAGATCATGCTCCAGGACATATTTGGAGAAGACTGTGTAAGTGTAAAG
  GATGACTCTATTCTTAGCGTCACAGTGGACGGGAAAACTGCCAACCTTAACTTGGAGACACGGACTGTAG
  AATGTGAAGAGGGAAGTGAAGACGATGAATCCCTCCGAGAAATGGTGGAGCTGGCTGCACAGAGACTGTA
  CGAGGCCCTGACGCCAGTTCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205834 representing NM_016207
  Red=Cloning site Green=Tags(s)
  
  MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLIDPAEIDLLLIS
  HFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINF
  HEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTH
  IHEKREEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSLAKKCMA
  VYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQSGLSRELFESWCTDKRN
  GVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGE
  QNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNF
  NYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLCYQLQKLTGDVEELEIQEKPALKVFKNITVIQEP
  GMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEIMLQDIFGEDCVSVK
  DDSILSVTVDGKTANLNLETRTVECEEGSEDDESLREMVELAAQRLYEALTPVH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016207
• ORF Size: 2052 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016207.2, NP_057291.1
• RefSeq Size: 2286
• RefSeq ORF: 2055
• Locus ID: 51692
• Domains: lactamase_B
• Gene Summary: This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]