SMAP2 (NM_022733) Human Tagged ORF Clone

CAT#: RG205875

SMAP2 (GFP-tagged) - Human small ArfGAP2 (SMAP2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SMAP2
• Synonyms: SMAP1L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205875 representing NM_022733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGGCAAGTCGGTGAAGGACGTGGATCGGTACCAGGCTGTCCTGGCCAACCTGCTGCTGGAGGAGG
  ATAACAAGTTTTGTGCAGATTGCCAGTCTAAAGGGCCGCGATGGGCCTCTTGGAACATTGGTGTGTTCAT
  CTGCATTCGATGTGCTGGAATCCACAGGAATCTGGGGGTGCACATATCCAGGGTAAAGTCAGTTAACCTC
  GACCAGTGGACTCAAGAACAGATTCAGTGCATGCAAGAGATGGGAAATGGAAAGGCAAACCGACTTTATG
  AAGCCTATCTTCCTGAGACCTTTCGGCGACCTCAGATAGACCCAGCTGTTGAAGGATTTATTCGAGACAA
  ATATGAGAAGAAGAAATACATGGACCGAAGTCTGGACATCAATGCCTTTAGGAAAGAAAAAGATGACAAG
  TGGAAAAGAGGGAGCGAACCAGTTCCAGAAAAAAAATTGGAACCTGTTGTTTTTGAGAAGGTGAAAATGC
  CACAGAAAAAAGAAGACCCACAGCTACCTCGGAAAAGCTCCCCGAAATCCACAGCGCCTGTCATGGATTT
  GTTGGGCCTTGATGCTCCTGTGGCCTGCTCCATTGCAAATAGTAAGACCAGCAATACCCTAGAGAAGGAT
  TTAGATCTGTTGGCCTCTGTTCCATCCCCTTCTTCTTCCGGTTCCAGAAAGGTTGTAGGTTCCATGCCAA
  CTGCAGGGAGTGCCGGCTCTGTTCCTGAAAATCTGAACCTGTTTCCGGAGCCAGGGAGCAAATCAGAAGA
  AATAGGCAAGAAACAGCTCTCTAAAGACTCCATTCTTTCACTGTATGGATCCCAGACGCCTCAAATGCCT
  ACTCAAGCAATGTTCATGGCTCCCGCTCAGATGGCATATCCCACAGCCTACCCCAGCTTCCCCGGGGTTA
  CACCTCCTAACAGCATAATGGGGAGCATGATGCCTCCACCAGTAGGCATGGTTGCTCAGCCAGGAGCTTC
  TGGGATGGTTGCCCCCATGGCCATGCCTGCAGGCTATATGGGTGGCATGCAGGCATCAATGATGGGTGTG
  CCGAATGGAATGATGACCACCCAGCAGGCTGGCTACATGGCAGGCATGGCAGCTATGCCCCAGACTGTGT
  ATGGGGTCCAGCCAGCTCAGCAGCTGCAATGGAACCTTACTCAGATGACCCAGCAGATGGCTGGGATGAA
  CTTCTATGGAGCCAATGGCATGATGAACTATGGACAGTCAATGAGTGGCGGAAATGGACAGGCAGCAAAT
  CAGACTCTCAGTCCTCAGATGTGGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205875 representing NM_022733
  Red=Cloning site Green=Tags(s)
  
  MTGKSVKDVDRYQAVLANLLLEEDNKFCADCQSKGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNL
  DQWTQEQIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINAFRKEKDDK
  WKRGSEPVPEKKLEPVVFEKVKMPQKKEDPQLPRKSSPKSTAPVMDLLGLDAPVACSIANSKTSNTLEKD
  LDLLASVPSPSSSGSRKVVGSMPTAGSAGSVPENLNLFPEPGSKSEEIGKKQLSKDSILSLYGSQTPQMP
  TQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGSMMPPPVGMVAQPGASGMVAPMAMPAGYMGGMQASMMGV
  PNGMMTTQQAGYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMSGGNGQAAN
  QTLSPQMWK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022733
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022733.1, NP_073570.1
• RefSeq Size: 2618
• RefSeq ORF: 1290
• Locus ID: 64744
• Domains: ArfGap
• Protein Pathways: Endocytosis
• Gene Summary: GTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network. [UniProtKB/Swiss-Prot Function]