Cytohesin 3 (CYTH3) (NM_004227) Human Tagged ORF Clone

CAT#: RG206003

CYTH3 (GFP-tagged) - Human cytohesin 3 (CYTH3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CYTH3
• Synonyms: ARNO3; cytohesin-3; GRP1; PSCD3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206003 representing NM_004227
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAGACGGCGGCGGCGAGGGTGGTGGCGTGCCTGAAGACCTCTCATTAGAAGAGAGAGAAGAAC
  TTCTAGACATTCGTCGAAGAAAAAAGGAACTTATTGATGACATTGAGAGGCTGAAATATGAAATTGCAGA
  GGTGATGACAGAGATCGACAATCTAACTTCCGTAGAGGAGAGCAAAACGACTCAGAGGAACAAACAGATA
  GCCATGGGAAGAAAGAAATTCAACATGGATCCCAAAAAGGGAATTCAGTTTCTAATAGAAAATGACCTGC
  TACAGAGTTCCCCAGAAGACGTCGCCCAGTTCCTTTATAAAGGAGAAGGCCTAAATAAGACCGTCATTGG
  GGACTACCTGGGTGAAAGGGATGAATTTAATATTAAAGTTCTTCAAGCCTTTGTTGAACTCCATGAGTTT
  GCTGATCTCAACCTTGTACAAGCCTTAAGGCAGTTCTTATGGAGCTTCAGGCTGCCCGGGGAGGCGCAGA
  AGATTGATCGCATGATGGAGGCTTTCGCTTCTCGCTACTGCCTGTGCAACCCCGGGGTCTTCCAGTCCAC
  AGACACGTGCTACGTGCTGTCATTCGCCATCATCATGCTCAACACCAGCCTCCACAACCACAACGTGCGT
  GACAAGCCCACGGCAGAACGGTTCATCGCCATGAACCGCGGCATCAACGAGGGCGGGGACCTCCCTGAGG
  AGCTGCTGAGGAATTTGTATGAGAGCATTAAGAACGAGCCATTTAAGATCCCGGAGGACGACGGGAACGA
  CCTGACCCACACCTTCTTCAACCCCGACCGCGAGGGCTGGCTCCTGAAGCTGGGAGGGCGTGTGAAGACC
  TGGAAGCGCCGGTGGTTCATCCTGACCGATAACTGCCTCTATTACTTTGAATACACAACAGATAAGGAGC
  CCAGGGGAATCATCCCGTTGGAAAACCTCAGCATCAGGGAGGTGGAGGACCCCCGGAAACCCAACTGTTT
  TGAGCTCTACAATCCCAGCCACAAAGGGCAGGTCATCAAGGCCTGTAAGACGGAGGCCGACGGCCGCGTG
  GTAGAGGGGAACCATGTGGTGTACCGGATCTCAGCCCCGAGCCCGGAGGAGAAGGAGGAGTGGATGAAAT
  CCATCAAAGCCAGTATCAGCAGAGATCCCTTCTATGACATGTTGGCAACGAGGAAACGAAGGATTGCCAA
  TAAAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206003 representing NM_004227
  Red=Cloning site Green=Tags(s)
  
  MDEDGGGEGGGVPEDLSLEEREELLDIRRRKKELIDDIERLKYEIAEVMTEIDNLTSVEESKTTQRNKQI
  AMGRKKFNMDPKKGIQFLIENDLLQSSPEDVAQFLYKGEGLNKTVIGDYLGERDEFNIKVLQAFVELHEF
  ADLNLVQALRQFLWSFRLPGEAQKIDRMMEAFASRYCLCNPGVFQSTDTCYVLSFAIIMLNTSLHNHNVR
  DKPTAERFIAMNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKT
  WKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSHKGQVIKACKTEADGRV
  VEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRKRRIANKK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004227
• ORF Size: 1197 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004227.3, NP_004218.1
• RefSeq Size: 4482
• RefSeq ORF: 1200
• Locus ID: 9265
• Domains: Sec7, PH
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. PSCD family members have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This encoded protein is involved in the control of Golgi structure and function, and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ARF1. [provided by RefSeq, Jul 2008]