Carbohydrate sulfotransferase 4 (CHST4) (NM_005769) Human Tagged ORF Clone

CAT#: RG206533

CHST4 (GFP-tagged) - Human carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4 (CHST4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHST4
• Synonyms: GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206533 representing NM_005769
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACTGCCTAAAAAAATGAAGCTCCTGCTGTTTCTGGTTTCCCAGATGGCCATCTTGGCTCTATTCT
  TCCACATGTACAGCCACAACATCAGCTCCCTGTCTATGAAGGCACAGCCCGAGCGCATGCACGTGCTGGT
  TCTGTCTTCCTGGCGCTCTGGCTCTTCTTTTGTGGGGCAGCTTTTTGGGCAGCACCCAGATGTTTTCTAC
  CTGATGGAGCCCGCCTGGCACGTGTGGATGACCTTCAAGCAGAGCACCGCCTGGATGCTGCACATGGCTG
  TGCGGGATCTGATACGGGCCGTCTTCTTGTGCGACATGAGCGTCTTTGATGCCTACATGGAACCTGGTCC
  CCGGAGACAGTCCAGCCTCTTTCAGTGGGAGAACAGCCGGGCCCTGTGTTCTGCACCTGCCTGTGACATC
  ATCCCACAAGATGAAATCATCCCCCGGGCTCACTGCAGGCTCCTGTGCAGTCAACAGCCCTTTGAGGTGG
  TGGAGAAGGCCTGCCGCTCCTACAGCCACGTGGTGCTCAAGGAGGTGCGCTTCTTCAACCTGCAGTCCCT
  CTACCCGCTGCTGAAAGACCCCTCCCTCAACCTGCATATCGTGCACCTGGTCCGGGACCCCCGGGCCGTG
  TTCCGTTCCCGAGAACGCACAAAGGGAGATCTCATGATTGACAGTCGCATTGTGATGGGGCAGCATGAGC
  AGAAACTCAAGAAGGAGGACCAACCCTACTATGTGATGCAGGTCATCTGCCAAAGCCAGCTGGAGATCTA
  CAAGACCATCCAGTCCTTGCCCAAGGCCCTGCAGGAACGCTACCTGCTTGTGCGCTATGAGGACCTGGCT
  CGAGCCCCTGTGGCCCAGACTTCCCGAATGTATGAATTCGTGGGATTGGAATTCTTGCCCCATCTTCAGA
  CCTGGGTGCATAACATCACCCGAGGCAAGGGCATGGGTGACCACGCTTTCCACACAAATGCCAGGGATGC
  CCTTAATGTCTCCCAGGCTTGGCGCTGGTCTTTGCCCTATGAAAAGGTTTCTCGACTTCAGAAAGCCTGT
  GGCGATGCCATGAATTTGCTGGGCTACCGCCACGTCAGATCTGAACAAGAACAGAGAAACCTGTTGCTGG
  ATCTTCTGTCTACCTGGACTGTCCCTGAGCAAATCCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206533 representing NM_005769
  Red=Cloning site Green=Tags(s)
  
  MLLPKKMKLLLFLVSQMAILALFFHMYSHNISSLSMKAQPERMHVLVLSSWRSGSSFVGQLFGQHPDVFY
  LMEPAWHVWMTFKQSTAWMLHMAVRDLIRAVFLCDMSVFDAYMEPGPRRQSSLFQWENSRALCSAPACDI
  IPQDEIIPRAHCRLLCSQQPFEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAV
  FRSRERTKGDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLA
  RAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKAC
  GDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005769
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005769.1, NP_005760.1
• RefSeq Size: 2032
• RefSeq ORF: 1161
• Locus ID: 10164
• Protein Families: Transmembrane
• Protein Pathways: Keratan sulfate biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]