Dihydropyrimidinase (DPYS) (NM_001385) Human Tagged ORF Clone

CAT#: RG206603

DPYS (GFP-tagged) - Human dihydropyrimidinase (DPYS)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DPYS
• Synonyms: DHP; DHPase
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206603 representing NM_001385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCTCGCGGCTCCTGATCCGCGGGGGTCGCGTGGTCAACGATGACTTCTCGGAGGTGGCCG
  ACGTGCTGGTGGAGGACGGCGTGGTGCGGGCACTCGGGCACGACCTGCTGCCTCCCGGGGGCGCTCCTGC
  GGGGCTGCGGGTCCTCGACGCCGCCGGCAAGCTCGTCCTGCCCGGAGGCATCGACACACACACGCACATG
  CAGTTCCCCTTCATGGGCTCGCGGTCCATCGACGACTTCCACCAGGGCACCAAGGCTGCTCTCTCAGGAG
  GCACCACCATGATTATTGATTTCGCCATTCCTCAGAAAGGTGGCTCCCTCATTGAGGCCTTCGAGACCTG
  GCGAAGCTGGGCTGATCCCAAAGTTTGCTGCGACTACAGCCTTCATGTGGCAGTGACGTGGTGGAGTGAC
  CAGGTTAAAGAAGAAATGAAAATCCTTGTGCAAGATAAAGGTGTTAACTCTTTCAAGATGTTTATGGCCT
  ATAAAGATCTGTACATGGTGACAGACCTGGAGCTGTACGAAGCCTTCTCTCGGTGCAAGGAAATTGGAGC
  AATTGCCCAGGTCCATGCGGAAAATGGAGACTTAATTGCAGAGGGAGCAAAGAAGATGTTGGCTCTGGGG
  ATAACAGGCCCTGAGGGCCACGAGCTGTGCCGCCCAGAGGCAGTGGAGGCAGAGGCCACGCTGAGAGCCA
  TCACCATAGCCAGCGCTGTGAACTGTCCTCTCTACATTGTGCATGTGATGAGCAAGTCTGCAGCTAAGGT
  GATAGCGGATGCAAGGAGAGATGGGAAGGTGGTCTATGGTGAACCCATAGCAGCCAGTCTTGGCACAGAT
  GGCACTCACTACTGGAATAAAGAATGGCACCATGCAGCCCACCATGTCATGGGTCCACCTTTGCGACCAG
  ACCCCTCAACACCCGACTTCCTCATGAATCTGTTGGCTAATGATGATCTAACCACAACAGGGACTGATAA
  CTGCACTTTCAACACCTGCCAGAAAGCTCTTGGGAAGGATGATTTTACCAAGATCCCCAATGGGGTGAAT
  GGTGTTGAAGATCGGATGTCCGTAATATGGGAAAAAGGCGTGCATAGTGGTAAAATGGATGAAAACAGAT
  TTGTGGCAGTTACCAGCACAAATGCAGCCAAAATTTTTAATCTCTATCCAAGAAAAGGAAGAATAGCTGT
  AGGATCAGATGCTGACATTGTTATTTGGGACCCAAAAGGCACAAGGACTATCTCAGCAAAAACTCATCAT
  CAGGCTGTTAACTTCAACATTTTCGAGGGCATGGTTTGCCACGGGGTGCCCCTTGTGACTATTTCAAGAG
  GCAAAGTGGTATATGAAGCCGGAGTGTTCAGTGTCACGGCAGGAGATGGGAAGTTTATTCCTCGAAAACC
  ATTTGCTGAATATATTTACAAACGAATAAAGCAGCGAGACCGGACTTGCACACCTACCCCTGTGGAGCGT
  GCACCCTATAAGGGAGAAGTCGCCACACTGAAATCCAGAGTGACAAAAGAAGATGCCACAGCAGGGACCA
  GGAAACAGGCCCACCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206603 representing NM_001385
  Red=Cloning site Green=Tags(s)
  
  MAAPSRLLIRGGRVVNDDFSEVADVLVEDGVVRALGHDLLPPGGAPAGLRVLDAAGKLVLPGGIDTHTHM
  QFPFMGSRSIDDFHQGTKAALSGGTTMIIDFAIPQKGGSLIEAFETWRSWADPKVCCDYSLHVAVTWWSD
  QVKEEMKILVQDKGVNSFKMFMAYKDLYMVTDLELYEAFSRCKEIGAIAQVHAENGDLIAEGAKKMLALG
  ITGPEGHELCRPEAVEAEATLRAITIASAVNCPLYIVHVMSKSAAKVIADARRDGKVVYGEPIAASLGTD
  GTHYWNKEWHHAAHHVMGPPLRPDPSTPDFLMNLLANDDLTTTGTDNCTFNTCQKALGKDDFTKIPNGVN
  GVEDRMSVIWEKGVHSGKMDENRFVAVTSTNAAKIFNLYPRKGRIAVGSDADIVIWDPKGTRTISAKTHH
  QAVNFNIFEGMVCHGVPLVTISRGKVVYEAGVFSVTAGDGKFIPRKPFAEYIYKRIKQRDRTCTPTPVER
  APYKGEVATLKSRVTKEDATAGTRKQAHP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001385
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001385.1, NP_001376.1
• RefSeq Size: 2123 bp
• RefSeq ORF: 1560 bp
• Locus ID: 1807
• Cytogenetics: 8q22.3
• Domains: Amidohydro_1
• Protein Families: Druggable Genome
• Protein Pathways: beta-Alanine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Pantothenate and CoA biosynthesis, Pyrimidine metabolism
• Gene Summary: 'Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria. [provided by RefSeq, Jul 2008]'