LDHD (NM_153486) Human Tagged ORF Clone

CAT#: RG206631

LDHD (GFP-tagged) - Human lactate dehydrogenase D (LDHD), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LDHD
• Synonyms: DLACD; DLD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206631 representing NM_153486
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGACTGCTCAGGTCTGCAACCTGGGAGCTGTTCCCCTGGAGGGGCTACTGCTCCCAGAAGGCAA
  AGGGAGAGCTCTGCAGGGACTTCGTAGAGGCTCTGAAGGCCGTGGTGGGCGGCTCCCACGTGTCCACTGC
  CGCGGTGGTCCGAGAGCAGCACGGGCGCGATGAGTCGGTGCACAGGTGCGAACCTCCTGATGCTGTGGTG
  TGGCCCCAGAACGTGGAGCAGGTCAGCCGGCTGGCAGCCCTGTGCTATCGCCAAGGTGTGCCCATCATCC
  CATTCGGCACCGGCACCGGGCTTGAGGGTGGCGTCTGTGCTGTGCAGGGCGGCGTCTGCGTTAACCTGAC
  GCATATGGACCGAATCCTGGAGCTGAACCAGGAGGACTTCTCTGTGGTGGTGGAGCCAGGTGTCACCCGC
  AAAGCCCTCAACGCCCACCTGCGGGACAGCGGCCTCTGGTTTCCCGTGGACCCAGGCGCGGACGCCTCTC
  TCTGTGGCATGGCGGCCACCGGGGCGTCGGGGACCAACGCGGTCCGCTACGGCACCATGCGGGACAACGT
  GCTCAACCTGGAGGTGGTGCTGCCCGACGGGCGGCTGCTGCACACGGCGGGCCGAGGCCGGCATTTCCGC
  TTCGGCTTCTGGCCAGAAATCCCTCATCACACAGCCTGGTACTCACCTTGTGTGTCCCTGGGACGTAGGA
  AGAGTGCAGCCGGCTACAACCTCACGGGGCTCTTCGTGGGCTCCGAGGGGACGCTGGGCCTCATCACAGC
  CACCACCCTGCGCCTGCACCCTGCCCCTGAGGCCACAGTGGCCGCCACGTGTGCGTTCCCCAGTGTCCAG
  GCTGCTGTGGACAGCACTGTACACATCCTCCAGGCTGCAGTGCCCGTAGCCCGCATTGAGTTCCTGGATG
  AAGTCATGATGGATGCCTGCAACAGGTACAGCAAGCTGAATTGCTTAGTGGCGCCCACACTCTTCCTGGA
  GTTCCATGGCTCCCAGCAGGCACTGGAGGAGCAGCTGCAGCGCACAGAGGAGATAGTCCAGCAGAACGGA
  GCCTCTGACTTCTCCTGGGCCAAGGAGGCCGAGGAGCGCAGCCGGCTTTGGACAGCACGGCACAATGCCT
  GGTACGCAGCCCTGGCCACGCGGCCAGGCTGCAAGGGCTACTCCACGGATGTGTGTGTGCCCATCTCCCG
  GCTGCCGGAGATCGTGGTGCAGACCAAGGAGGATCTGAATGCCTCAGGACTCACAGGAAGCATTGTCGGG
  CATGTGGGTGACGGCAACTTCCACTGCATCCTGCTGGTCAACCCTGATGACGCCGAGGAACTGGGCAGGG
  TCAAGGCTTTTGCAGAACAGCTGGGCAGGCGGGCACTGGCTCTCCACGGAACGTGCACGGGGGAGCATGG
  CATCGGAATGGGCAAGCGGCAGCTGCTGCAGGAGGAGGTGGGCGCCGTGGGCGTGGAGACCATGCGGCAG
  CTCAAGGCCGTGCTAGACCCCCAAGGCCTCATGAATCCAGGCAAAGTGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206631 representing NM_153486
  Red=Cloning site Green=Tags(s)
  
  MARLLRSATWELFPWRGYCSQKAKGELCRDFVEALKAVVGGSHVSTAAVVREQHGRDESVHRCEPPDAVV
  WPQNVEQVSRLAALCYRQGVPIIPFGTGTGLEGGVCAVQGGVCVNLTHMDRILELNQEDFSVVVEPGVTR
  KALNAHLRDSGLWFPVDPGADASLCGMAATGASGTNAVRYGTMRDNVLNLEVVLPDGRLLHTAGRGRHFR
  FGFWPEIPHHTAWYSPCVSLGRRKSAAGYNLTGLFVGSEGTLGLITATTLRLHPAPEATVAATCAFPSVQ
  AAVDSTVHILQAAVPVARIEFLDEVMMDACNRYSKLNCLVAPTLFLEFHGSQQALEEQLQRTEEIVQQNG
  ASDFSWAKEAEERSRLWTARHNAWYAALATRPGCKGYSTDVCVPISRLPEIVVQTKEDLNASGLTGSIVG
  HVGDGNFHCILLVNPDDAEELGRVKAFAEQLGRRALALHGTCTGEHGIGMGKRQLLQEEVGAVGVETMRQ
  LKAVLDPQGLMNPGKVL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153486
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153486.3, NP_705690.2
• RefSeq Size: 2096
• RefSeq ORF: 1524
• Locus ID: 197257
• Protein Pathways: Pyruvate metabolism
• Gene Summary: The protein encoded by this gene belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The similar protein in yeast has both D-lactate and D-glycerate dehydrogenase activities. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]