NEI3 (NEIL3) (NM_018248) Human Tagged ORF Clone

CAT#: RG206838

NEIL3 (GFP-tagged) - Human nei endonuclease VIII-like 3 (E. coli) (NEIL3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NEIL3
• Synonyms: FGP2; FPG2; hFPG2; hNEI3; NEI3; ZGRF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206838 representing NM_018248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAAGGACCAGGCTGTACTCTGAATGGAGAGAAGATTCGAGCGCGGGTGCTCCCGGGCCAGGCGG
  TGACCGGCGTGCGGGGAAGCGCTCTGCGGAGTCTGCAGGGCCGCGCCTTGCGGCTCGCAGCCTCCACGGT
  TGTGGTCTCCCCGCAGGCTGCTGCACTGAATAATGATTCCAGCCAGAATGTCTTGAGCCTGTTTAATGGA
  TATGTTTACAGTGGCGTGGAAACTTTGGGGAAGGAGCTCTTTATGTACTTTGGACCAAAAGCTTTACGGA
  TTCATTTCGGAATGAAAGGCTTCATCATGATTAATCCACTTGAGTATAAATATAAAAATGGAGCTTCTCG
  TGTTTTGGAAGTGCAGCTCACCAAAGATTTGATTTGTTTCTTTGACTCATCAGTAGAACTCAGAAACTCA
  ATGGAAAGCCAACAGAGAATAAGAATGATGAAAGAATTAGATGTATGTTCACCTGAATTTAGTTTCTTGA
  GAGCAGAAAGTGAAGTTAAAAAACAGAAAGGCCGGATGCTAGGTGATGTGCTAATGGATCAGAACGTATT
  GCCTGGAGTAGGGAACATCATCAAAAATGAAGCTCTCTTTGACAGTGGTCTCCACCCAGCTGTTAAAGTT
  TGTCAATTAACAGATGAACAGATCCATCACCTCATGAAAATGATACGTGATTTCAGCATTCTCTTTTACA
  GGTGCCGTAAAGCAGGACTTGCTCTCTCTAAACACTATAAGGTTTACAAGCGTCCCAATTGTGGTCAGTG
  CCACTGCAGAATAACTGTGTGCCGCTTTGGGGACAATAACAGAATGACATATTTCTGTCCTCACTGTCAA
  AAAGAAAATCCTCAACATGTTGACATATGCAAGCTACCGACTAGAAATACTATAATCAGTTGGACATCTA
  GCAGGGTGGATCATGTTATGGACTCCGTGGCTCGGAAGTCGGAAGAGCACTGGACCTGTGTGGTGTGTAC
  TTTAATCAATAAGCCCTCTTCTAAGGCATGTGATGCTTGCTTGACCTCAAGGCCTATTGATTCAGTGCTC
  AAGAGTGAAGAAAATTCTACTGTCTTTAGCCACTTAATGAAGTACCCGTGTAATACTTTTGGAAAACCTC
  ATACAGAAGTCAAGATCAACAGGAAAACTGCATTTGGAACTACAACTCTTGTCTTGACTGATTTTAGCAA
  TAAATCCAGTACTTTGGAAAGAAAAACAAAGCAAAACCAGATACTAGATGAGGAGTTTCAAAACTCTCCT
  CCTGCTAGTGTGTGTTTGAATGATATACAGCACCCCTCCAAGAAGACAACAAACGATATAACTCAACTAT
  CCAGCAAAGTAAACATATCACCTACAATCAGTTCAGAATCTAAATTATTTAGTCCAGCACATAAAAAACC
  GAAAACAGCCCACTACTCATCACCAGAGCTTAAAAGCTGCAACCCTGGATATTCTAACAGTGAACTTCAA
  ATTAATATGACAGATGGCCCTCGTACCTTAAATCCTGACAGCCCTCGCTGCAGTAAACACAACCGCCTCT
  GCATTCTCCGAGTTGTGAGGAAGGATGGGGAAAACAAGGGCAGGCAGTTTTATGCCTGTCCTCTACCTAG
  AGAAGCACAATGTGGATTTTTTGAATGGGCAGATTTGTCCTTCCCATTCTGCAACCATGGCAAGCGTTCC
  ACCATGAAAACAGTATTGAAGATTGGACCTAACAATGGAAAGAATTTTTTTGTGTGTCCTCTTGGGAAGG
  AAAAACAATGCAATTTTTTCCAGTGGGCAGAAAATGGGCCAGGAATAAAAATTATTCCTGGATGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206838 representing NM_018248
  Red=Cloning site Green=Tags(s)
  
  MVEGPGCTLNGEKIRARVLPGQAVTGVRGSALRSLQGRALRLAASTVVVSPQAAALNNDSSQNVLSLFNG
  YVYSGVETLGKELFMYFGPKALRIHFGMKGFIMINPLEYKYKNGASRVLEVQLTKDLICFFDSSVELRNS
  MESQQRIRMMKELDVCSPEFSFLRAESEVKKQKGRMLGDVLMDQNVLPGVGNIIKNEALFDSGLHPAVKV
  CQLTDEQIHHLMKMIRDFSILFYRCRKAGLALSKHYKVYKRPNCGQCHCRITVCRFGDNNRMTYFCPHCQ
  KENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVL
  KSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSP
  PASVCLNDIQHPSKKTTNDITQLSSKVNISPTISSESKLFSPAHKKPKTAHYSSPELKSCNPGYSNSELQ
  INMTDGPRTLNPDSPRCSKHNRLCILRVVRKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRS
  TMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018248
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018248.1, NP_060718.1
• RefSeq Size: 2273
• RefSeq ORF: 1818
• Locus ID: 55247
• Domains: Fapy_DNA_glyco, zf-RanBP
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]). [supplied by OMIM, Mar 2008]