TFE3 (NM_006521) Human Tagged ORF Clone

CAT#: RG206840

TFE3 (GFP-tagged) - Human transcription factor binding to IGHM enhancer 3 (TFE3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TFE3
• Synonyms: bHLHe33; RCCP2; RCCX1; TFEA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206840 representing NM_006521
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCATGCGGCCGAACCAGCTCGGGATGGCGTAGAGGCCAGCGCGGAGGGCCCTCGAGCCGTGTTCG
  TGCTGTTGGAGGAGCGCAGGCCGGCCGACTCGGCTCAGCTGCTCAGCCTGAACTCTTTGCTTCCGGAATC
  CGGGATTGTTGCTGACATAGAATTAGAAAACGTCCTTGATCCTGACAGCTTCTACGAGCTCAAAAGCCAA
  CCCTTACCCCTTCGCTCAAGCCTCCCAATATCACTGCAGGCCACACCAGCCACCCCAGCTACACTCTCTG
  CATCGTCTTCTGCAGGGGGCTCCAGGACCCCTGCCATGTCGTCATCTTCTTCATCGAGGGTCTTGCTGCG
  GCAGCAGCTAATGCGGGCCCAGGCGCAGGAGCAGGAGAGGCGTGAGCGTCGGGAACAGGCCGCCGCGGCT
  CCCTTCCCCAGTCCTGCACCTGCCTCTCCTGCCATCTCTGTGGTTGGCGTCTCTGCTGGGGGCCACACAT
  TGAGCCGTCCACCCCCTGCTCAGGTGCCCAGGGAGGTGCTCAAGGTGCAGACCCATCTGGAGAACCCAAC
  GCGCTACCACCTGCAGCAGGCGCGCCGGCAGCAGGTGAAACAGTACCTGTCCACCACACTCGGGCCCAAG
  CTGGCTTCCCAGGCCCTCACCCCACCGCCGGGGCCCGCAAGTGCCCAGCCACTGCCTGCCCCTGAGGCTG
  CCCACACTACCGGCCCCACAGGCAGTGCGCCCAACAGCCCCATGGCGCTGCTCACCATCGGGTCCAGCTC
  AGAGAAGGAGATTGATGATGTCATTGATGAGATCATCAGCCTGGAGTCCAGTTACAATGATGAAATGCTC
  AGCTATCTGCCCGGAGGCACCACAGGACTGCAGCTCCCCAGCACGCTGCCTGTGTCAGGGAATCTGCTTG
  ATGTGTACAGTAGTCAAGGCGTGGCCACACCAGCCATCACTGTCAGCAACTCCTGCCCAGCTGAGCTGCC
  CAACATCAAACGGGAGATCTCTGAGACCGAGGCAAAGGCCCTTTTGAAGGAACGGCAGAAGAAAGACAAT
  CACAACCTAATTGAGCGTCGCAGGCGATTCAACATTAACGACAGGATCAAGGAACTGGGCACTCTCATCC
  CTAAGTCCAGTGACCCGGAGATGCGCTGGAACAAGGGCACCATCCTGAAGGCCTCTGTGGATTATATCCG
  CAAGCTGCAGAAGGAGCAGCAGCGCTCCAAAGACCTGGAGAGCCGGCAGCGATCCCTGGAGCAGGCCAAC
  CGCAGCCTGCAGCTCCGAATTCAGGAACTAGAACTGCAGGCCCAGATCCATGGCCTGCCAGTGCCTCCCA
  CTCCAGGGCTGCTTTCCTTGGCCACGACTTCGGCTTCTGACAGCCTCAAGCCAGAGCAGCTGGACATTGA
  GGAGGAGGGCAGGCCAGGCGCAGCAACGTTCCATGTAGGGGGGGGACCTGCCCAGAATGCTCCCCATCAG
  CAGCCCCCTGCACCGCCCTCAGATGCCCTTCTGGACCTGCACTTTCCCAGCGACCACCTGGGGGACCTGG
  GAGACCCCTTCCACCTGGGGCTGGAGGACATTCTGATGGAGGAGGAGGAGGGGGTGGTGGGAGGACTGTC
  GGGGGGTGCCCTGTCCCCACTGCGGGCTGCCTCCGATCCCCTGCTCTCTTCAGTGTCCCCTGCTGTCTCC
  AAGGCCAGCAGCCGCCGCAGCAGCTTCAGCATGGAAGAGGAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206840 representing NM_006521
  Red=Cloning site Green=Tags(s)
  
  MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQ
  PLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAA
  PFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPK
  LASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEML
  SYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDN
  HNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQAN
  RSLQLRIQELELQAQIHGLPVPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAATFHVGGGPAQNAPHQ
  QPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLRAASDPLLSSVSPAVS
  KASSRRSSFSMEEES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006521
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006521.3, NP_006512.2
• RefSeq Size: 3431 bp
• RefSeq ORF: 1728 bp
• Locus ID: 7030
• Cytogenetics: Xp11.23
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]'