TFE3 (NM_006521) Human Untagged Clone

CAT#: SC116014

TFE3 (untagged)-Human transcription factor binding to IGHM enhancer 3 (TFE3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFE3
• Synonyms: bHLHe33; RCCP2; RCCX1; TFEA
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006521, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTCATGCGGCCGAACCAGCTCGGGATGGCGTAGAGGCCAGCGCGGAGGGCCCTCGAGCCGTGTTCG
  TGCTGTTGGAGGAGCGCAGGCCGGCCGACTCGGCTCAGCTGCTCAGCCTGAACTCTTTGCTTCCGGAATC
  CGGGATTGTTGCTGACATAGAATTAGAAAACGTCCTTGATCCTGACAGCTTCTACGAGCTCAAAAGCCAA
  CCCTTACCCCTTCGCTCAAGCCTCCCAATATCACTGCAGGCCACACCAGCCACCCCAGCTACACTCTCTG
  CATCGTCTTCTGCAGGGGGCTCCAGGACCCCTGCCATGTCGTCATCTTCTTCATCGAGGGTCTTGCTGCG
  GCAGCAGCTAATGCGGGCCCAGGCGCAGGAGCAGGAGAGGCGTGAGCGTCGGGAACAGGCCGCCGCGGCT
  CCCTTCCCCAGTCCTGCACCTGCCTCTCCTGCCATCTCTGTGGTTGGCGTCTCTGCTGGGGGCCACACAT
  TGAGCCGTCCACCCCCTGCTCAGGTGCCCAGGGAGGTGCTCAAGGTGCAGACCCATCTGGAGAACCCAAC
  GCGCTACCACCTGCAGCAGGCGCGCCGGCAGCAGGTGAAACAGTACCTGTCCACCACACTCGGGCCCAAG
  CTGGCTTCCCAGGCCCTCACCCCACCGCCGGGGCCCGCAAGTGCCCAGCCACTGCCTGCCCCTGAGGCTG
  CCCACACTACCGGCCCCACAGGCAGTGCGCCCAACAGCCCCATGGCGCTGCTCACCATCGGGTCCAGCTC
  AGAGAAGGAGATTGATGATGTCATTGATGAGATCATCAGCCTGGAGTCCAGTTACAATGATGAAATGCTC
  AGCTATCTGCCCGGAGGCACCACAGGACTGCAGCTCCCCAGCACGCTGCCTGTGTCAGGGAATCTGCTTG
  ATGTGTACAGTAGTCAAGGCGTGGCCACACCAGCCATCACTGTCAGCAACTCCTGCCCAGCTGAGCTGCC
  CAACATCAAACGGGAGATCTCTGAGACCGAGGCAAAGGCCCTTTTGAAGGAACGGCAGAAGAAAGACAAT
  CACAACCTAATTGAGCGTCGCAGGCGATTCAACATTAACGACAGGATCAAGGAACTGGGCACTCTCATCC
  CTAAGTCCAGTGACCCGGAGATGCGCTGGAACAAGGGCACCATCCTGAAGGCCTCTGTGGATTATATCCG
  CAAGCTGCAGAAGGAGCAGCAGCGCTCCAAAGACCTGGAGAGCCGGCAGCGATCCCTGGAGCAGGCCAAC
  CGCAGCCTGCAGCTCCGAATTCAGGAACTAGAACTGCAGGCCCAGATCCATGGCCTGCCAGTACCTCCCA
  CTCCAGGGCTGCTTTCCTTGGCCACGACTTCGGCTTCTGACAGCCTCAAGCCAGAGCAGCTGGACATTGA
  GGAGGAGGGCAGGCCAGGCGCAGCAACGTTCCATGTAGGGGGGGGACCTGCCCAGAATGCTCCCCATCAG
  CAGCCCCCTGCACCGCCCTCAGATGCCCTTCTGGACCTGCACTTTCCCAGCGACCACCTGGGGGACCTGG
  GAGACCCCTTCCACCTGGGGCTGGAGGACATTCTGATGGAGGAGGAGGAGGGGGTGGTGGGAGGACTGTC
  GGGGGGTGCCCTGTCCCCACTGCGGGCTGCCTCCGATCCCCTGCTCTCTTCAGTGTCCCCTGCTGTCTCC
  AAGGCCAGCAGCCGCCGCAGCAGCTTCAGCATGGAAGAGGAGTCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006521 unedited
  ATTACCCCGCCCCGTTGCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG
  AATTCGGCACGAGGAGGGGGGGCGTCGGTCCGTTCTGGGCGGGGGATGACTCACAGCCCA
  TCCCATCTCCCCGACGCCGCCCGCCCGCGCAGTGCTAGCTCCATGGCTTAGCGGAGGAGG
  CGGCAGTGGCGAGCTGGGGGGAGGGGGGACTCTTATTTTGTTAGGGGGACCGGGCCGAGG
  CCCGACCGGCCTGGCAGGGCTCGCCCGGGGCCGGGCGTCATGTCTCATGCGGCCGAACCA
  GCTCGGGATGGCGTAGAGGCCAGCGCGGAGGGCCCTCGAGCCGTGTTCGTGCTGTTGGAG
  GAGCGCAGGCCGGCCGACTCGGCTCAGCTGCTCAGCCTGAACTCTTTGCTTCCGGAATCC
  GGGATTGTTGCTGACATAGAATTAGAAAACGTCCTTGATCCTGACAGCTTCTACGAGCTC
  AAAAGCCAACCCTTACCCCTTCGCTCAAGCCTCCCAATATCACTGCAGGCCACACCAGCC
  ACCCCAGCTACACTCTCTGCATCGTCTTCTGCAGGGGGCTCCAGGACCCCTGCCATGTCG
  TCATCTTCTTCATCGAGGGTCTTGCTGCGGCAGCAGCTAATGCTGGCCCAGGCGCAGGAG
  CAGGAGAGGCGTGAGCTGTCGGCACAGGCCGCCGCGGCTCCCTTCCCCAGTCTGCACCTG
  CCTCTCCTGCCTCTCATGTGGCTGGCGTCTCTGCTGGGGGCCACACATGAGCCGTCCACC
  CCTGCTCAGTGCCCCAGGGAGTGCTCAAGGTGCAGACCATCTGGAGAACCTACGCGCTAC
  CACTGCAGCAGCGCGCTGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006521 unedited
  TATGGTACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCAAACACTTCC
  TATCTTTTATTATTTTTAATCACAAATTTTTTTACCCCTCATTTTACCCTCCATGGGGCC
  TGGCCCCAGCAATCCTTATCTAACACCCTTGGTTGTAAGTATGATGGGTGGAAAAGGAGG
  GGGGAGTCCCCCATTTCCCCAAAGGCCCCGGTTGGGTCTTTTTTTTCAAAAAAACCATTT
  TTTTTAAAAAATTCTGGAACCCCTTTTGGCCCCTGAACCCCCCCCCCCCCTTTTTTTTTA
  AAACTGGGGCTAAAAAAAGCAAGCCCCAACCAATTTTTAATTCCAAAAAACCCAGGGTTT
  AAAAAGTCCCGGTTTACCCCGGGAAATTCAGGGGATTTTTGGAAAAAAAAAAAAGGGGCT
  TTTTCCCCTTTTCCCCGGGCAAAAAAATTTTCCCCAGGGCCCACCCAAGTTTTTTTTTCC
  TTTTTCCTGGGGGGAAGGCCCCCTGGGGAAACTGGGAAAATTCTTTTCACCCCTTTTTGA
  AAAAATCAACACCTTTTTTTTTCCGGGAACCCCCATTGGAAGGGCCCGGGCTTGTAAAGG
  CCCAAGGTTTTTCCCCCCAAAAATGCCCCCTTTGGAATTTTGGGCCCCCAAAAACCTTGC
  CCTCGGGTCCGAAGTTTTTATTAAAACCCCGGGGACCCCCAAGGTTTAATGGGGGGGGGC
  CTCTTAAGGTGGGGGGGGCCCGCGGGACCCCCCCCTCACTTTTGGGGATATTTTCTTAGA
  GGAAAAAGGGACTTTTGCCCCCAGGGAAAACCCTTCCTGCACCCTAAATTTGTTTAAAAA
  TTTTGGGACCCGTTTCCCCCCCCCGCCTCCTGGAAAGGGCCCTCCACCCCGTTGGGGTTT
  GTTTTTTCGGTGAAAACAAAACCCGGGGACCCTTGTGCCAAAACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006521
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006521.3, NP_006512.2
• RefSeq Size: 3431 bp
• RefSeq ORF: 1728 bp
• Locus ID: 7030
• Cytogenetics: Xp11.23
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).