HIBADH (NM_152740) Human Tagged ORF Clone

CAT#: RG207125

HIBADH (GFP-tagged) - Human 3-hydroxyisobutyrate dehydrogenase (HIBADH)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HIBADH
• Synonyms: NS5ATP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207125 representing NM_152740
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCCTTACGGCTCCTCGGAGCTGCCTCCGGTCTCCGGTACTGGAGCCGGCGGCTGCGGCCGG
  CAGCCGGCAGCTTTGCAGCGGTGTGTTCTAGGTCAGTGGCTTCAAAGACTCCAGTTGGATTCATTGGACT
  GGGCAACATGGGGAATCCAATGGCAAAAAATCTCATGAAACATGGCTATCCACTTATTATTTATGATGTG
  TTCCCTGATGCCTGCAAAGAGTTTCAAGATGCAGGTGAACAGGTAGTATCTTCCCCAGCAGATGTTGCTG
  AAAAAGCTGACAGAATTATTACAATGCTGCCCACCAGTATCAATGCAATAGAAGCTTATTCCGGAGCAAA
  TGGGATTCTAAAAAAAGTGAAGAAGGGCTCATTATTAATAGATTCCAGCACTATTGATCCTGCAGTTTCA
  AAAGAATTGGCCAAAGAAGTTGAGAAAATGGGAGCAGTTTTCATGGATGCCCCTGTTTCTGGTGGTGTAG
  GAGCTGCACGATCTGGGAACCTCACGTTTATGGTGGGAGGAGTTGAAGATGAATTTGCTGCTGCCCAAGA
  GTTGCTGGGGTGCATGGGCTCCAACGTGGTGTACTGTGGAGCTGTTGGGACTGGGCAGGCGGCAAAGATC
  TGCAACAACATGCTGTTAGCTATTAGTATGATTGGAACTGCTGAAGCTATGAATCTTGGAATCAGGTTAG
  GGCTTGACCCAAAACTACTGGCTAAAATCCTAAATATGAGCTCAGGACGGTGTTGGTCAAGTGACACTTA
  TAATCCTGTACCTGGAGTGATGGATGGCGTTCCCTCGGCTAATAACTATCAGGGTGGATTTGGAACAACA
  CTCATGGCTAAGGATCTGGGATTGGCACAAGACTCTGCTACCAGCACAAAGAGCCCAATCCTTCTTGGCA
  GTCTGGCCCATCAGATCTACAGGATGATGTGTGCAAAGGGCTACTCAAAGAAAGACTTCTCATCCGTGTT
  CCAGTTCCTACGAGAGGAGGAGACCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207125 representing NM_152740
  Red=Cloning site Green=Tags(s)
  
  MAASLRLLGAASGLRYWSRRLRPAAGSFAAVCSRSVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDV
  FPDACKEFQDAGEQVVSSPADVAEKADRIITMLPTSINAIEAYSGANGILKKVKKGSLLIDSSTIDPAVS
  KELAKEVEKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQAAKI
  CNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGTT
  LMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKDFSSVFQFLREEETF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152740
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152740.2, NP_689953.1
• RefSeq Size: 2006
• RefSeq ORF: 1011
• Locus ID: 11112
• Domains: NAD_binding_2
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011]