PIGU (NM_080476) Human Tagged ORF Clone

CAT#: RG207146

PIGU (GFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class U (PIGU)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIGU
• Synonyms: CDC91L1; GAB1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207146 representing NM_080476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCCCTTGGTCCTGGTGCTGGTGGTGGCTGTGACAGTGCGGGCGGCCTTGTTCCGCTCCAGTC
  TGGCCGAGTTCATTTCCGAGCGGGTGGAGGTGGTGTCCCCACTGAGCTCTTGGAAGAGAGTGGTTGAAGG
  CCTTTCACTGTTGGACTTGGGAGTATCTCCGTATTCTGGAGCAGTATTTCATGAAACTCCATTAATAATA
  TACCTCTTTCATTTCCTAATTGACTATGCTGAATTGGTGTTTATGATAACTGATGCACTCACTGCTATTG
  CCCTGTATTTTGCAATCCAGGACTTCAATAAAGTTGTGTTTAAAAAGCAGAAACTCCTCCTAGAACTGGA
  CCAGTATGCCCCAGATGTGGCCGAACTCATCCGGACCCCTATGGAAATGCGTTACATCCCTTTGAAAGTG
  GCCCTGTTCTATCTCTTAAATCCTTACACGATTTTGTCTTGTGTTGCCAAGTCTACCTGTGCCATCAACA
  ACACCCTCATTGCTTTCTTCATTTTGACTACGATAAAAGGCAGTGCTTTCCTCAGTGCTATTTTTCTTGC
  CTTAGCGACATACCAGTCTCTGTACCCACTCACCTTGTTTGTCCCAGGACTCCTCTATCTCCTCCAGCGG
  CAGTACATACCTGTGAAAATGAAGAGCAAAGCCTTCTGGATCTTTTCTTGGGAGTATGCCATGATGTATG
  TGGGAAGCCTAGTGGTAATCATTTGCCTCTCCTTCTTCCTTCTCAGCTCTTGGGATTTCATCCCCGCAGT
  CTATGGCTTTATACTTTCTGTTCCAGATCTCACTCCAAACATTGGTCTTTTCTGGTACTTCTTTGCAGAG
  ATGTTTGAGCACTTCAGCCTCTTCTTTGTATGTGTGTTTCAGATCAACGTCTTCTTCTACACCATCCCCT
  TAGCCATAAAGCTAAAGGAGCACCCCATCTTCTTCATGTTTATCCAGATCGCTGTCATCGCCATCTTTAA
  GTCCTACCCGACAGTGGGGGACGTGGCGCTCTACATGGCCTTCTTCCCCGTGTGGAACCATCTCTACAGA
  TTCCTGAGAAACATCTTTGTCCTCACCTGCATCATCATCGTCTGTTCCCTGCTCTTCCCTGTCCTGTGGC
  ACCTCTGGATTTATGCAGGAAGTGCCAACTCTAATTTCTTTTATGCCATCACACTGACCTTCAACGTTGG
  GCAGATCCTGCTCATCTCTGATTACTTCTATGCCTTCCTGCGGCGGGAGTACTACCTCACACATGGCCTC
  TACTTGACCGCCAAGGATGGCACAGAGGCCATGCTCGTGCTCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207146 representing NM_080476
  Red=Cloning site Green=Tags(s)
  
  MAAPLVLVLVVAVTVRAALFRSSLAEFISERVEVVSPLSSWKRVVEGLSLLDLGVSPYSGAVFHETPLII
  YLFHFLIDYAELVFMITDALTAIALYFAIQDFNKVVFKKQKLLLELDQYAPDVAELIRTPMEMRYIPLKV
  ALFYLLNPYTILSCVAKSTCAINNTLIAFFILTTIKGSAFLSAIFLALATYQSLYPLTLFVPGLLYLLQR
  QYIPVKMKSKAFWIFSWEYAMMYVGSLVVIICLSFFLLSSWDFIPAVYGFILSVPDLTPNIGLFWYFFAE
  MFEHFSLFFVCVFQINVFFYTIPLAIKLKEHPIFFMFIQIAVIAIFKSYPTVGDVALYMAFFPVWNHLYR
  FLRNIFVLTCIIIVCSLLFPVLWHLWIYAGSANSNFFYAITLTFNVGQILLISDYFYAFLRREYYLTHGL
  YLTAKDGTEAMLVLK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080476
• ORF Size: 1305 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_080476.4, NP_536724.1
• RefSeq Size: 1833
• RefSeq ORF: 1308
• Locus ID: 128869
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Cdc91, a predicted integral membrane protein that may function in cell division control. The protein encoded by this gene is the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins. [provided by RefSeq, Jul 2008]