iASPP (PPP1R13L) (NM_006663) Human Tagged ORF Clone

CAT#: RG207150

PPP1R13L (GFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 13 like (PPP1R13L), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPP1R13L
• Synonyms: IASPP; NKIP1; RAI; RAI4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207150 representing NM_006663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCGAGGCATTCCAGAGCGCGCGGGACTTTCTGGACATGAACTTCCAGTCGCTGGCCATGAAAC
  ACATGGATCTGAAGCAGATGGAGCTGGACACGGCGGCGGCCAAGGTGGATGAACTGACCAAGCAGCTGGA
  GTCGCTGTGGTCAGACTCTCCCGCGCCTCCTGGCCCGCAGGCCGGACCCCCTTCTAGGCCGCCCCGGTAC
  AGCTCCAGCTCGATCCCTGAGCCCTTCGGCAGCCGAGGGTCCCCCCGGAAGGCGGCCACCGACGGCGCAG
  ACACCCCGTTCGGACGATCAGAGAGTGCCCCAACCCTACACCCCTACAGCCCGCTGTCCCCCAAGGGACG
  GCCGTCGTCGCCGCGCACCCCGCTCTACCTGCAGCCGGACGCCTACGGCAGCCTGGACCGCGCGACCTCG
  CCCCGGCCCCGCGCCTTCGATGGCGCAGGCAGCTCCCTCGGCCGTGCGCCCTCCCCGCGGCCCGGGCCAG
  GCCCGCTCCGCCAGCAGGGTCCCCCCACGCCTTTCGACTTCCTGGGCCGCGCAGGCTCCCCCCGCGGCAG
  CCCCCTGGCGGAGGGGCCCCAGGCCTTCTTCCCCGAGCGTGGGCCGTCACCGCGCCCCCCTGCCACAGCC
  TACGACGCGCCAGCGTCCGCCTTCGGGAGCTCCCTGCTAGGCTCCGGCGGCAGCGCATTCGCCCCGCCTC
  TGCGCGCGCAAGACGACCTGACGCTGCGCCGGCGGCCTCCGAAAGCCTGGAACGAGTCTGACCTGGACGT
  GGCGTACGAGAAGAAGCCTTCGCAGACAGCGAGCTATGAACGCCTGGACGTCTTCGCAAGGCCTGCCTCG
  CCGAGCCTGCAGCTGTTGCCTTGGAGGGAGAGCAGCCTGGATGGACTGGGGGGCACCGGCAAGGACAACC
  TCACTAGCGCCACCCTGCCGCGCAATTACAAGGTCTCTCCTCTGGCCAGCGACCGGCGTTCAGACGCGGG
  CAGCTACCGGCGCTCGCTGGGCTCCGCGGGGCCGTCGGGCACTTTGCCTCGCAGCTGGCAGCCCGTCAGC
  CGCATCCCCATGCCCCCCTCCAGCCCCCAGCCCCGCGGGGCCCCGCGCCAGCGTCCCATCCCCCTCAGCA
  TGATCTTCAAGCTGCAGAACGCCTTCTGGGAGCACGGGGCCAGCCGCGCCATGCTCCCTGGGTCCCCCCT
  CTTCACCCGAGCACCCCCGCCTAAGCTGCAGCCCCAACCACAACCACAGCCCCAGCCACAATCACAACCA
  CAGCCCCAGCTGCCCCCACAGCCCCAGACCCAACCCCAAACCCCTACCCCAGCCCCCCAGCATCCCCAAC
  AGACATGGCCCCCTGTGAACGAAGGACCCCCCAAACCCCCCACCGAGCTGGAGCCTGAGCCGGAGATAGA
  GGGGCTGCTGACACCAGTGCTGGAGGCTGGCGATGTGGATGAAGGCCCTGTAGCAAGGCCTCTCAGCCCC
  ACGAGGCTGCAGCCAGCACTGCCACCGGAGGCACAGTCGGTGCCCGAGCTGGAGGAGGTGGCACGGGTGT
  TGGCGGAAATTCCCCGGCCCCTCAAACGCAGGGGCTCCATGGAGCAGGCCCCTGCTGTGGCCCTGCCCCC
  TACCCACAAGAAACAGTACCAGCAGATCATCAGCCGCCTCTTCCATCGTCATGGGGGGCCAGGGCCCGGG
  GGGCCGGAGCCAGAGCTGTCCCCCATCACTGAGGGATCTGAGGCCAGGGCAGGGCCCCCTGCTCCTGCCC
  CACCAGCTCCCATTCCACCCCCGGCCCCGTCCCAGAGCAGCCCACCAGAGCAGCCGCAGAGCATGGAGAT
  GCGCTCTGTGCTGCGGAAGGCGGGCTCCCCGCGCAAGGCCCGCCGCGCGCGCCTCAACCCTCTGGTGCTC
  CTCCTGGACGCGGCGCTGACCGGGGAGCTGGAGGTGGTGCAGCAGGCGGTGAAGGAGATGAACGACCCGA
  GCCAGCCCAACGAGGAGGGCATCACTGCCTTGCACAACGCCATCTGCGGCGCCAACTACTCTATCGTGGA
  TTTCCTCATCACCGCGGGTGCCAATGTCAACTCCCCCGACAGCCACGGCTGGACACCCTTGCACTGCGCG
  GCGTCGTGCAACGACACAGTCATCTGCATGGCGCTGGTGCAGCACGGCGCTGCAATCTTCGCCACCACGC
  TCAGCGACGGCGCCACCGCCTTCGAGAAGTGCGACCCTTACCGCGAGGGTTATGCTGACTGCGCCACCTA
  CCTGGCAGACGTCGAGCAGAGTATGGGGCTGATGAACAGCGGGGCAGTGTACGCTCTCTGGGACTACAGC
  GCCGAGTTCGGGGACGAGCTGTCCTTCCGCGAGGGCGAGTCGGTCACCGTGCTGCGGAGGGACGGGCCGG
  AGGAGACCGACTGGTGGTGGGCCGCGCTGCACGGCCAGGAGGGCTACGTGCCGCGGAACTACTTCGGGCT
  GTTCCCCAGGGTGAAGCCTCAAAGGAGTAAAGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207150 representing NM_006663
  Red=Cloning site Green=Tags(s)
  
  MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRY
  SSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATS
  PRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFPERGPSPRPPATA
  YDAPASAFGSSLLGSGGSAFAPPLRAQDDLTLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPAS
  PSLQLLPWRESSLDGLGGTGKDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVS
  RIPMPPSSPQPRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQP
  QPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVDEGPVARPLSP
  TRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHKKQYQQIISRLFHRHGGPGPG
  GPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPEQPQSMEMRSVLRKAGSPRKARRARLNPLVL
  LLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCA
  ASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYS
  AEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006663
• ORF Size: 2484 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006663.2, NP_006654.2
• RefSeq Size: 3117
• RefSeq ORF: 2487
• Locus ID: 10848
• Domains: SH3, ANK
• Protein Families: Transcription Factors
• Gene Summary: IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53. [supplied by OMIM, Mar 2008]