PAK6 (NM_020168) Human Tagged ORF Clone

CAT#: RG207438

PAK6 (GFP-tagged) - Human p21 protein (Cdc42/Rac)-activated kinase 6 (PAK6), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PAK6
• Synonyms: PAK5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207438 representing NM_020168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGCAAGAAAAAGAAGAAACGCCCTGAGATCTCAGCGCCACAGAACTTCCAGCACCGTGTCCACA
  CCTCCTTCGACCCCAAAGAAGGCAAGTTTGTGGGCCTCCCCCCACAATGGCAGAACATCCTGGACACACT
  GCGGCGCCCCAAGCCCGTGGTGGACCCTTCGCGAATCACACGGGTGCAGCTCCAGCCCATGAAGACAGTG
  GTGCGGGGCAGCGCGATGCCTGTGGATGGCTACATCTCGGGGCTGCTCAACGACATCCAGAAGTTGTCAG
  TCATCAGCTCCAACACCCTGCGTGGCCGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCT
  GGGGGATGAGCACTGGGCCACCGACCCAGACATGTACCTCCAGAGCCCCCAGTCTGAGCGCACTGACCCC
  CACGGCCTCTACCTCAGCTGCAACGGGGGCACACCAGCAGGCCACAAGCAGATGCCGTGGCCCGAGCCAC
  AGAGCCCACGGGTCCTGCCCAATGGGCTGGCTGCAAAGGCACAGTCCCTGGGCCCCGCCGAGTTTCAGGG
  TGCCTCGCAGCGCTGTCTGCAGCTGGGTGCCTGCCTGCAGAGCTCCCCACCAGGAGCCTCGCCCCCCACG
  GGCACCAATAGGCATGGAATGAAGGCTGCCAAGCATGGCTCTGAGGAGGCCCGGCCACAGTCCTGCCTGG
  TGGGCTCAGCCACAGGCAGGCCAGGTGGGGAAGGCAGCCCTAGCCCTAAGACCCGGGAGAGCAGCCTGAA
  GCGCAGGCTATTCCGAAGCATGTTCCTGTCCACTGCTGCCACAGCCCCTCCAAGCAGCAGCAAGCCAGGC
  CCTCCACCACAGAGCAAGCCCAACTCCTCTTTCCGACCGCCGCAGAAAGACAACCCCCCAAGCCTGGTGG
  CCAAGGCCCAGTCCTTGCCCTCGGACCAGCCGGTGGGGACCTTCAGCCCTCTGACCACTTCGGATACCAG
  CAGCCCCCAGAAGTCCCTCCGCACAGCCCCGGCCACAGGCCAGCTTCCAGGCCGGTCTTCCCCAGCGGGA
  TCCCCCCGCACCTGGCACGCCCAGATCAGCACCAGCAACCTGTACCTGCCCCAGGACCCCACGGTTGCCA
  AGGGTGCCCTGGCTGGTGAGGACACAGGTGTTGTGACACATGAGCAGTTCAAGGCTGCGCTCAGGATGGT
  GGTGGACCAGGGTGACCCCCGGCTGCTGCTGGACAGCTACGTGAAGATTGGCGAGGGCTCCACCGGCATC
  GTCTGCTTGGCCCGGGAGAAGCACTCGGGCCGCCAGGTGGCCGTCAAGATGATGGACCTCAGGAAGCAGC
  AGCGCAGGGAGCTGCTCTTCAACGAGGTGGTGATCATGCGGGACTACCAGCACTTCAACGTGGTGGAGAT
  GTACAAGAGCTACCTGGTGGGCGAGGAGCTGTGGGTGCTCATGGAGTTCCTGCAGGGAGGAGCCCTCACA
  GACATCGTCTCCCAAGTCAGGCTGAATGAGGAGCAGATTGCCACTGTGTGTGAGGCTGTGCTGCAGGCCC
  TGGCCTACCTGCATGCTCAGGGTGTCATCCACCGGGACATCAAGAGTGACTCCATCCTGCTGACCCTCGA
  TGGCAGGGTGAAGCTCTCGGACTTCGGATTCTGTGCTCAGATCAGCAAAGACGTCCCTAAGAGGAAGTCC
  CTGGTGGGAACCCCCTACTGGATGGCTCCTGAAGTGATCTCCAGGTCTTTGTATGCCACTGAGGTGGATA
  TCTGGTCTCTGGGCATCATGGTGATTGAGATGGTAGATGGGGAGCCACCGTACTTCAGTGACTCCCCAGT
  GCAAGCCATGAAGAGGCTCCGGGACAGCCCCCCACCCAAGCTGAAAAACTCTCACAAGGTCTCCCCAGTG
  CTGCGAGACTTCCTGGAGCGGATGCTGGTGCGGGACCCCCAAGAGAGAGCCACAGCCCAGGAGCTCCTAG
  ACCACCCCTTCCTGCTGCAGACAGGGCTACCTGAGTGCCTGGTGCCCCTGATCCAGCTCTACCGAAAGCA
  GACCTCCACCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207438 representing NM_020168
  Red=Cloning site Green=Tags(s)
  
  MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTV
  VRGSAMPVDGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDP
  HGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPT
  GTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFRSMFLSTAATAPPSSSKPG
  PPPQSKPNSSFRPPQKDNPPSLVAKAQSLPSDQPVGTFSPLTTSDTSSPQKSLRTAPATGQLPGRSSPAG
  SPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGI
  VCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALT
  DIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKS
  LVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPV
  LRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020168
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020168.3, NP_064553.1
• RefSeq Size: 3889
• RefSeq ORF: 2046
• Locus ID: 56924
• Domains: PBD, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: This gene encodes a member of a family of p21-stimulated serine/threonine protein kinases, which contain an amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. These kinases function in a number of cellular processes, including cytoskeleton rearrangement, apoptosis, and the mitogen-activated protein (MAP) kinase signaling pathway. The protein encoded by this gene interacts with androgen receptor (AR) and translocates to the nucleus, where it is involved in transcriptional regulation. Changes in expression of this gene have been linked to prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]