MBD2 (NM_003927) Human Tagged ORF Clone

CAT#: RG207511

MBD2 (GFP-tagged) - Human methyl-CpG binding domain protein 2 (MBD2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MBD2
• Synonyms: DMTase; NY-CO-41
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207511 representing NM_003927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCGCACCCGGGGGGAGGCCGCTGCTGCCCGGAGCAGGAGGAGGGGGAGAGTGCGGCGGGCGGCA
  GCGGCGCTGGCGGCGACTCCGCCATAGAGCAGGGGGGCCAGGGCAGCGCGCTCGCCCCGTCCCCGGTGAG
  CGGCGTGCGCAGGGAAGGCGCTCGGGGCGGCGGCCGTGGCCGGGGGCGGTGGAAGCAGGCGGGCCGGGGC
  GGCGGCGTCTGTGGCCGTGGCCGGGGCCGGGGCCGTGGCCGGGGACGGGGACGGGGCCGGGGCCGGGGCC
  GCGGCCGTCCCCCGAGTGGCGGCAGCGGCCTTGGCGGCGACGGCGGCGGCTGCGGCGGCGGCGGCAGCGG
  TGGCGGCGGCGCCCCCCGGCGGGAGCCGGTCCCTTTCCCGTCGGGGAGCGCGGGGCCGGGGCCCAGGGGA
  CCCCGGGCCACGGAGAGCGGGAAGAGGATGGATTGCCCGGCCCTCCCCCCCGGATGGAAGAAGGAGGAAG
  TGATCCGAAAATCTGGGCTAAGTGCTGGCAAGAGCGATGTCTACTACTTCAGTCCAAGTGGTAAGAAGTT
  CAGAAGCAAGCCTCAGTTGGCAAGGTACCTGGGAAATACTGTTGATCTCAGCAGTTTTGACTTCAGAACT
  GGAAAGATGATGCCTAGTAAATTACAGAAGAACAAACAGAGACTGCGAAACGATCCTCTCAATCAAAATA
  AGGGTAAACCAGACTTGAATACAACATTGCCAATTAGACAAACAGCATCAATTTTCAAACAACCGGTAAC
  CAAAGTCACAAATCATCCTAGTAATAAAGTGAAATCAGACCCACAACGAATGAATGAACAGCCACGTCAG
  CTTTTCTGGGAGAAGAGGCTACAAGGACTTAGTGCATCAGATGTAACAGAACAAATTATAAAAACCATGG
  AACTACCCAAAGGTCTTCAAGGAGTTGGTCCAGGTAGCAATGATGAGACCCTTTTATCTGCTGTTGCCAG
  TGCTTTGCACACAAGCTCTGCGCCAATCACAGGGCAAGTCTCCGCTGCTGTGGAAAAGAACCCTGCTGTT
  TGGCTTAACACATCTCAACCCCTCTGCAAAGCTTTTATTGTCACAGATGAAGACATCAGGAAACAGGAAG
  AGCGAGTACAGCAAGTACGCAAGAAATTGGAAGAAGCACTGATGGCAGACATCTTGTCGCGAGCTGCTGA
  TACAGAAGAGATGGATATTGAAATGGACAGTGGAGATGAAGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207511 representing NM_003927
  Red=Cloning site Green=Tags(s)
  
  MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAGRG
  GGVCGRGRGRGRGRGRGRGRGRGRGRPPSGGSGLGGDGGGCGGGGSGGGGAPRREPVPFPSGSAGPGPRG
  PRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNTVDLSSFDFRT
  GKMMPSKLQKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKVTNHPSNKVKSDPQRMNEQPRQ
  LFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSAPITGQVSAAVEKNPAV
  WLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADILSRAADTEEMDIEMDSGDEA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003927
• ORF Size: 1233 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003927.3, NP_003918.1
• RefSeq Size: 2584
• RefSeq ORF: 1236
• Locus ID: 8932
• Domains: MBD
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]