MBD2 (NM_003927) Human Untagged Clone

CAT#: SC321741

MBD2 (untagged)-Human methyl-CpG binding domain protein 2 (MBD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MBD2
• Synonyms: DMTase; NY-CO-41
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003927.3 CCACGCGTCCGCCGGGATTCCAAGGGCTCGGTTACGGAAGAAGCGCAGCGCCGGCTGGGG
  AGGGGGCTGGATGCGCGCGCACCCGGGGGGAGGCCGCTGCTGCCCGGAGCAGGAGGAGGG
  GGAGAGTGCGGCGGGCGGCAGCGGCGCTGGCGGCGACTCCGCCATAGAGCAGGGGGGCCA
  GGGCAGCGCGCTCGCCCCGTCCCCGGTGAGCGGCGTGCGCAGGGAAGGCGCTCGGGGCGG
  CGGCCGTGGCCGGGGGCGGTGGAAGCAGGCGGGCCGGGGCGGCGGCGTCTGTGGCCGTGG
  CCGGGGCCGGGGCCGTGGCCGGGGACGGGGACGGGGCCGGGGCCGGGGCCGCGGCCGTCC
  CCCGAGTGGCGGCAGCGGCCTTGGCGGCGACGGCGGCGGCTGCGGCGGCGGCGGCAGCGG
  TGGCGGCGGCGCCCCCCGGCGGGAGCCGGTCCCTTTCCCGTCGGGGAGCGCGGGGCCGGG
  GCCCAGGGGACCCCGGGCCACGGAGAGCGGGAAGAGGATGGATTGCCCGGCCCTCCCCCC
  CGGATGGAAGAAGGAGGAAGTGATCCGAAAATCTGGGCTAAGTGCTGGCAAGAGCGATGT
  CTACTACTTCAGTCCAAGTGGTAAGAAGTTCAGAAGCAAGCCTCAGTTGGCAAGGTACCT
  GGGAAATACTGTTGATCTCAGCAGTTTTGACTTCAGAACTGGAAAGATGATGCCTAGTAA
  ATTACAGAAGAACAAACAGAGACTGCGAAACGATCCTCTCAATCAAAATAAGGGTAAACC
  AGACTTGAATACAACATTGCCAATTAGACAAACAGCATCAATTTTCAAACAACCGGTAAC
  CAAAGTCACAAATCATCCTAGTAATAAAGTGAAATCAGACCCACAACGAATGAATGAACA
  GCCACGTCAGCTTTTCTGGGAGAAGAGGCTACAAGGACTTAGTGCATCAGATGTAACAGA
  ACAAATTATAAAAACCATGGAACTACCCAAAGGTCTTCAAGGAGTTGGTCCAGGTAGCAA
  TGATGAGACCCTTTTATCTGCTGTTGCCAGTGCTTTGCACACAAGCTCTGCGCCAATCAC
  AGGGCAAGTCTCCGCTGCTGTGGAAAAGAACCCTGCTGTTTGGCTTAACACATCTCAACC
  CCTCTGCAAAGCTTTTATTGTCACAGATGAAGACATCAGGAAACAGGAAGAGCGAGTACA
  GCAAGTACGCAAGAAATTGGAAGAAGCACTGATGGCAGACATCTTGTCGCGAGCTGCTGA
  TACAGAAGAGATGGATATTGAAATGGACAGTGGAGATGAAGCCTAAGAATATGATCAGGT
  AACTTTCGACCGACTTTCCCCAAGAGAAAATTCCTAGAAATTGAACAAAAATGTTTCCAC
  TGGCTTTTGCCTGTAAGAAAAAAAATGTACCCGAGCACATAGAGCTTTTTAATAGCACTA
  ACCAATGCCTTTTTAGATGTATTTTTGATGTATATATCTATTATTCAAAAAATCATGTTT
  ATTTTGAGTCCTAGGACTTAAAATTAGTCTTTTGTAATATCAAGCAGGACCCTAAGATGA
  AGCTGAGCTTTTGATGCCAGGTGCAATCTACTGGAAATGTAGCACTTACGTAAAACATTT
  GTTTCCCCCACAGTTTTAATAAGAACAGATCAGGAATTCTAAATAAATTTCCCAGTTAAA
  GATTATTGTGACTTCACTGTATATAAACATATTTTTATACTTTATTGAAAGGGGACACCT
  GTACATTCTTCCATCATCACTGTAAAGACAAATAAATGATTATATTCACAAAAAAAAAAA
  AAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003927.3 unedited
  GGTACATTGTATACGACTCCTATAGGGCGGGGCACGCGGCGCGCCGGTACCGGTCCGGAA
  GCCCGGGATATCGTCGACCCACGCGTCCGCCGGGATTCCAAGGGCTCGGTTACGGAAGAA
  GCGCAGCGCCGGCTGGGGAGGGGGCTGGATGCGCGCGCACCCGGGGGGAGGCCGCTGCTG
  CCCGGAGCAGGAGGAGGGGGAGAGTGCGGCGGGCGGCAGCGGCGCTGGCGGCGACTCCGC
  CATAGAGCAGGGGGGCCAGGGCAGCGCGCTCGCCCCGTCCCCGGTGAGCGGCGTGCGCAG
  GGAAGGCGCTCGGGGCGGTGGTTTTTTTTCGGGGGCGGTGGAAGCAGGCGGGCCGGGGCG
  GCGGCGTCTGTGGCCGTGGCCGGGGCCGGGGCCGTGGCCGGGGACGGGGACGGGGCCGGG
  GCCGGGGCCGCGGCCGTCCCCCGAGTGGCGGCAGCGGCCTTGGCGGCGACGGCGGCGGCT
  GCGGCGGCGGCGGCAGCGGTGGCGGCGGCGCCCCCCGGCGGGAGCCGGTCCCTTTCCCGT
  CGGGGAGCGCGGGGCCGGGGCCCAGGGGACCCCGGGCCACGGAGAGCGGGAAGAGGATGG
  ATTGCCCGGCCCTCCCCCCCGGATGGAAGAGGAGGAAGTGATCCGAAAATCTGGGCTAAG
  TGCTGGCAAGAGCGATGTCTACTACTTTCAGTCCAAGTGGTAAGAAGTTCAGGAGCAAGC
  CTCAGTTGGCAAAGGTACCTGGGAAATACTGTTGATCCTCAGCAGTTTGACTTCAGAACT
  GGGAAAGATGATGCTAGTAATTACGGAGACAAACGAGACTGCGAACGATCTCTCATTCAA
  AATAGGTAAACCCGACTTGATACAACATGGCCCATTAAACAAACAGCATTCATTTTCGAA
  ACAACCGGTTAAC
• Restriction Sites: Please inquire
• ACCN: NM_003927
• ORF Size: 1236 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_003927.3, NP_003918.1
• RefSeq Size: 2584
• RefSeq ORF: 1236
• Locus ID: 8932
• Domains: MBD
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.