Glycoprotein 2 (GP2) (NM_001007242) Human Tagged ORF Clone

CAT#: RG207512

GP2 (GFP-tagged) - Human glycoprotein 2 (zymogen granule membrane) (GP2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GP2
• Synonyms: ZAP75
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207512 representing NM_001007242
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGATGGTGGGCTCTGGCCTCCTGTGGCTGGCCTTGGTCTCCTGCATTCTGACCCAGGCATCTG
  CAGTGCAGCGAGACCCATCCACTGTGGAGGACAAGTGTGAGAAGGCCTGCCGCCCCGAGGAGGAGTGCCT
  TGCCCTCAACAGCACCTGGGGCTGTTTCTGCAGACAGGACCTCAATAGTTCTGATGTCCACAGTTTGCAG
  CCTCAGCTAGACTGTGGGCCCAGGGAGATCAAGGTGAAGGTGGACAAATGTTTGCTGGGAGGCCTGGGTT
  TGGGGGAGGAGGTCATTGCCTACCTGCGAGACCCAAACTGCAGCAGCATCTTGCAGACAGAGGAGAGGAA
  CTGGGTATCTGTGACCAGCCCCGTCCAGGCTAGTGCCTGCAGGAACATTCTGGAGAGAAATCAAACCCAT
  GCCATCTACAAAAACACCCTCTCCTTGGTCAATGATTTCATCATCAGAGACACCATCCTCAACATCAACT
  TCCAATGTGCCTACCCACTGGACATGAAAGTCAGCCTCCAAGCTGCCTTGCAGCCCATTGTAAGTTCCCT
  GAACGTCAGTGTGGACGGGAATGGAGAGTTCATTGTCAGGATGGCCCTCTTCCAAGACCAGAACTACACG
  AATCCTTACCAAGGGGATGCAGTTGAACTGTCTGTTGAGTCCGTGCTGTATGTGGGTGCCATCTTGGAAC
  AAGGGGACACCTCCCGGTTTAACCTGGTGTTGAGGAACTGCTATGCCACCCCCACTGAAGACAAGGCTGA
  CCTTGTGAAGTATTTCATCATCAGAAACAGCTGCTCAAATCAACGTGATTCCACCATCCACGTGGAGGAG
  AATGGGCAGTCCTCGGAAAGCCGGTTCTCAGTTCAGATGTTCATGTTTGCTGGACATTATGACCTAGTTT
  TCCTGCATTGTGAGATTCATCTCTGTGATTCTCTTAATGAACAGTGCCAGCCTTCTTGCTCAAGAAGTCA
  AGTCCGCAGTGAAGTACCGGCCATCGACCTAGCCCGGGTTCTAGATTTGGGGCCCATCACTCGGAGAGGT
  GCACAGTCTCCCGGTGTCATGAATGGAACCCCTAGCACTGCAGGGTTCCTGGTGGCCTGGCCTATGGTCC
  TCCTGACTGTCCTCCTGGCTTGGCTGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207512 representing NM_001007242
  Red=Cloning site Green=Tags(s)
  
  MERMVGSGLLWLALVSCILTQASAVQRDPSTVEDKCEKACRPEEECLALNSTWGCFCRQDLNSSDVHSLQ
  PQLDCGPREIKVKVDKCLLGGLGLGEEVIAYLRDPNCSSILQTEERNWVSVTSPVQASACRNILERNQTH
  AIYKNTLSLVNDFIIRDTILNINFQCAYPLDMKVSLQAALQPIVSSLNVSVDGNGEFIVRMALFQDQNYT
  NPYQGDAVELSVESVLYVGAILEQGDTSRFNLVLRNCYATPTEDKADLVKYFIIRNSCSNQRDSTIHVEE
  NGQSSESRFSVQMFMFAGHYDLVFLHCEIHLCDSLNEQCQPSCSRSQVRSEVPAIDLARVLDLGPITRRG
  AQSPGVMNGTPSTAGFLVAWPMVLLTVLLAWLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007242
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007242.1, NP_001007243.2
• RefSeq Size: 1998 bp
• RefSeq ORF: 1164 bp
• Locus ID: 2813
• Cytogenetics: 16p12.3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. The encoded protein binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]'