MRPS15 (NM_031280) Human Tagged ORF Clone

CAT#: RG207694

MRPS15 (GFP-tagged) - Human mitochondrial ribosomal protein S15 (MRPS15), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MRPS15
• Synonyms: DC37; MPR-S15; RPMS15; S15mt
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207694 representing NM_031280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGGTCGCGTGGAGGACGCTGAGTTTGATTCGGACCCGGGCAGTTACCCAGGTCCTAGTACCCG
  GGCTGCCGGGCGGTGGGAGCGCCAAGTTTCCTTTCAACCAGTGGGGCCTGCAGCCTCGAAGTCTCCTCCT
  CCAGGCCGCGCGCGGATATGTCGTCCGGAAACCAGCCCAGTCTAGGCTGGATGATGACCCACCTCCTTCT
  ACGCTGCTCAAAGACTACCAGAATGTCCCTGGAATTGAGAAGGTTGATGATGTCGTGAAAAGACTCTTGT
  CTTTGGAAATGGCCAACAAGAAGGAGATGCTAAAAATCAAGCAAGAACAGTTTATGAAGAAGATTGTTGC
  AAACCCAGAGGACACCAGATCCCTGGAGGCTCGAATTATTGCCTTGTCTGTCAAGATCCGCAGTTATGAA
  GAACACTTGGAGAAACATCGAAAGGACAAAGCCCACAAACGCTATCTGCTAATGAGCATTGACCAGAGGA
  AAAAGATGCTCAAAAACCTCCGTAACACCAACTATGATGTCTTTGAGAAGATATGCTGGGGGCTGGGAAT
  TGAGTACACCTTCCCCCCTCTGTATTACCGAAGAGCCCACCGCCGATTCGTGACCAAGAAGGCTCTGTGC
  ATTCGGGTTTTCCAGGAGACTCAAAAGCTGAAGAAGCGAAGAAGAGCCTTAAAGGCTGCAGCAGCAGCCC
  AAAAACAAGCAAAGCGGAGGAACCCAGACAGCCCTGCCAAAGCCATACCAAAGACACTCAAAGACAGCCA
  A
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207694 representing NM_031280
  Red=Cloning site Green=Tags(s)
  
  MLRVAWRTLSLIRTRAVTQVLVPGLPGGGSAKFPFNQWGLQPRSLLLQAARGYVVRKPAQSRLDDDPPPS
  TLLKDYQNVPGIEKVDDVVKRLLSLEMANKKEMLKIKQEQFMKKIVANPEDTRSLEARIIALSVKIRSYE
  EHLEKHRKDKAHKRYLLMSIDQRKKMLKNLRNTNYDVFEKICWGLGIEYTFPPLYYRRAHRRFVTKKALC
  IRVFQETQKLKKRRRALKAAAAAQKQAKRRNPDSPAKAIPKTLKDSQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031280
• ORF Size: 771 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031280.2, NP_112570.2
• RefSeq Size: 891
• RefSeq ORF: 774
• Locus ID: 64960
• Domains: Ribosomal_S15
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q. [provided by RefSeq, Jul 2008]