Interferon regulatory factor 9 (IRF9) (NM_006084) Human Tagged ORF Clone

CAT#: RG207768

IRF9 (GFP-tagged) - Human interferon regulatory factor 9 (IRF9)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IRF9
• Synonyms: IRF-9; ISGF3; ISGF3G; p48
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207768 representing NM_006084
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCAGGCAGGGCACGCTGCACCCGAAAACTCCGGAACTGGGTGGTGGAGCAAGTGGAGAGTGGGC
  AGTTTCCCGGAGTGTGCTGGGATGATACAGCTAAGACCATGTTCCGGATTCCCTGGAAACATGCAGGCAA
  GCAGGACTTCCGGGAGGACCAGGATGCTGCCTTCTTCAAGGCCTGGGCAATATTTAAGGGAAAGTATAAG
  GAGGGGGACACAGGAGGTCCAGCTGTCTGGAAGACTCGCCTGCGCTGTGCACTCAACAAGAGTTCTGAAT
  TTAAGGAGGTTCCTGAGAGGGGCCGCATGGATGTTGCTGAGCCCTACAAGGTGTATCAGTTGCTGCCACC
  AGGAATCGTCTCTGGCCAGCCAGGGACTCAGAAAGTACCATCAAAGCGACAGCACAGTTCTGTGTCCTCT
  GAGAGGAAGGAGGAAGAGGATGCCATGCAGAACTGCACACTCAGTCCCTCTGTGCTCCAGGACTCCCTCA
  ATAATGAGGAGGAGGGGGCCAGTGGGGGAGCAGTCCATTCAGACATTGGGAGCAGCAGCAGCAGCAGCAG
  CCCTGAGCCACAGGAAGTTACAGACACAACTGAGGCCCCCTTTCAAGGGGATCAGAGGTCCCTGGAGTTT
  CTGCTTCCTCCAGAGCCAGACTACTCACTGCTGCTCACCTTCATCTACAACGGGCGCGTGGTGGGCGAGG
  CCCAGGTGCAAAGCCTGGATTGCCGCCTTGTGGCTGAGCCCTCAGGCTCTGAGAGCAGCATGGAGCAGGT
  GCTGTTCCCCAAGCCTGGCCCACTGGAGCCCACGCAGCGCCTGCTGAGCCAGCTTGAGAGGGGCATCCTA
  GTGGCCAGCAACCCCCGAGGCCTCTTCGTGCAGCGCCTTTGCCCCATCCCCATCTCCTGGAATGCACCCC
  AGGCTCCACCTGGGCCAGGCCCGCATCTGCTGCCCAGCAACGAGTGCGTGGAGCTCTTCAGAACCGCCTA
  CTTCTGCAGAGACTTGGTCAGGTACTTTCAGGGCCTGGGCCCCCCACCGAAGTTCCAGGTAACACTGAAT
  TTCTGGGAAGAGAGCCATGGCTCCAGCCATACTCCACAGAATCTTATCACAGTGAAGATGGAGCAGGCCT
  TTGCCCGATACTTGCTGGAGCAGACTCCAGAGCAGCAGGCAGCCATTCTGTCCCTGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207768 representing NM_006084
  Red=Cloning site Green=Tags(s)
  
  MASGRARCTRKLRNWVVEQVESGQFPGVCWDDTAKTMFRIPWKHAGKQDFREDQDAAFFKAWAIFKGKYK
  EGDTGGPAVWKTRLRCALNKSSEFKEVPERGRMDVAEPYKVYQLLPPGIVSGQPGTQKVPSKRQHSSVSS
  ERKEEEDAMQNCTLSPSVLQDSLNNEEEGASGGAVHSDIGSSSSSSSPEPQEVTDTTEAPFQGDQRSLEF
  LLPPEPDYSLLLTFIYNGRVVGEAQVQSLDCRLVAEPSGSESSMEQVLFPKPGPLEPTQRLLSQLERGIL
  VASNPRGLFVQRLCPIPISWNAPQAPPGPGPHLLPSNECVELFRTAYFCRDLVRYFQGLGPPPKFQVTLN
  FWEESHGSSHTPQNLITVKMEQAFARYLLEQTPEQQAAILSLV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006084
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006084.4, NP_006075.3
• RefSeq Size: 1699
• RefSeq ORF: 1182
• Locus ID: 10379
• Domains: IRF
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway
• Gene Summary: Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. [UniProtKB/Swiss-Prot Function]