PAFAH (PLA2G7) (NM_005084) Human Tagged ORF Clone

CAT#: RG207776

PLA2G7 (GFP-tagged) - Human phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) (PLA2G7), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PLA2G7
• Synonyms: LDL-PLA2; LP-PLA2; PAFAD; PAFAH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207776 representing NM_005084
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCACCCAAATTGCATGTGCTTTTCTGCCTCTGCGGCTGCCTGGCTGTGGTTTATCCTTTTGACT
  GGCAATACATAAATCCTGTTGCCCATATGAAATCATCAGCATGGGTCAACAAAATACAAGTACTGATGGC
  TGCTGCAAGCTTTGGCCAAACTAAAATCCCCCGGGGAAATGGGCCTTATTCCGTTGGTTGTACAGACTTA
  ATGTTTGATCACACTAATAAGGGCACCTTCTTGCGTTTATATTATCCATCCCAAGATAATGATCGCCTTG
  ACACCCTTTGGATCCCAAATAAAGAATATTTTTGGGGTCTTAGCAAATTTCTTGGAACACACTGGCTTAT
  GGGCAACATTTTGAGGTTACTCTTTGGTTCAATGACAACTCCTGCAAACTGGAATTCCCCTCTGAGGCCT
  GGTGAAAAATATCCACTTGTTGTTTTTTCTCATGGTCTTGGGGCATTCAGGACACTTTATTCTGCTATTG
  GCATTGACCTGGCATCTCATGGGTTTATAGTTGCTGCTGTAGAACACAGAGATAGATCTGCATCTGCAAC
  TTACTATTTCAAGGACCAATCTGCTGCAGAAATAGGGGACAAGTCTTGGCTCTACCTTAGAACCCTGAAA
  CAAGAGGAGGAGACACATATACGAAATGAGCAGGTACGGCAAAGAGCAAAAGAATGTTCCCAAGCTCTCA
  GTCTGATTCTTGACATTGATCATGGAAAGCCAGTGAAGAATGCATTAGATTTAAAGTTTGATATGGAACA
  ACTGAAGGACTCTATTGATAGGGAAAAAATAGCAGTAATTGGACATTCTTTTGGTGGAGCAACGGTTATT
  CAGACTCTTAGTGAAGATCAGAGATTCAGATGTGGTATTGCCCTGGATGCATGGATGTTTCCACTGGGTG
  ATGAAGTATATTCCAGAATTCCTCAGCCCCTCTTTTTTATCAACTCTGAATATTTCCAATATCCTGCTAA
  TATCATAAAAATGAAAAAATGCTACTCACCTGATAAAGAAAGAAAGATGATTACAATCAGGGGTTCAGTC
  CACCAGAATTTTGCTGACTTCACTTTTGCAACTGGCAAAATAATTGGACACATGCTCAAATTAAAGGGAG
  ACATAGATTCAAATGCAGCTATTGATCTTAGCAACAAAGCTTCATTAGCATTCTTACAAAAGCATTTAGG
  ACTTCATAAAGATTTTGATCAGTGGGACTGCTTGATTGAAGGAGATGATGAGAATCTTATTCCAGGGACC
  AACATTAACACAACCAATCAACACATCATGTTACAGAACTCTTCAGGAATAGAGAAATACAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207776 representing NM_005084
  Red=Cloning site Green=Tags(s)
  
  MVPPKLHVLFCLCGCLAVVYPFDWQYINPVAHMKSSAWVNKIQVLMAAASFGQTKIPRGNGPYSVGCTDL
  MFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRP
  GEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLK
  QEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVI
  QTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKCYSPDKERKMITIRGSV
  HQNFADFTFATGKIIGHMLKLKGDIDSNAAIDLSNKASLAFLQKHLGLHKDFDQWDCLIEGDDENLIPGT
  NINTTNQHIMLQNSSGIEKYN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005084
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005084.2, NP_005075.2
• RefSeq Size: 1561
• RefSeq ORF: 1326
• Locus ID: 7941
• Domains: PAF-AH_p_II
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Ether lipid metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2009]