SIGLECL1 (SIGLEC12) (NM_053003) Human Tagged ORF Clone

CAT#: RG207808

SIGLEC12 (GFP-tagged) - Human sialic acid binding Ig-like lectin 12 (SIGLEC12), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SIGLEC12
• Synonyms: S2V; Siglec-XII; SIGLECL1; SLG
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207808 representing NM_053003
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACTGCTGCTGCTACTGCTGCCACCCCTGCTCTGTGGGAGAGTGGGGGCTAAGGAACAGAAGGATT
  ACCTGCTGACAATGCAGAAGTCCGTGACGGTGCAGGAGGGCCTGTGTGTCTCTGTGCTTTGCTCCTTCTC
  CTACCCCCAAAATGGCTGGACTGCCTCCGATCCAGTTCATGGCTACTGGTTCCGGGCAGGGGACCATGTA
  AGCCGGAACATTCCAGTGGCCACAAACAACCCAGCTCGAGCAGTGCAGGAGGAGACTCGGGACCGATTCC
  ACCTCCTTGGGGACCCACAGAACAAGGATTGTACCCTGAGCATCAGAGACACCAGAGAGAGTGATGCAGG
  GACATACGTCTTTTGTGTAGAGAGAGGAAATATGAAATGGAATTATAAATATGACCAGCTCTCTGTGAAT
  GTGACAGCGTCCCAGGACCTACTGTCAAGATACAGGCTGGAGGTGCCAGAGTCGGTGACTGTGCAGGAGG
  GTCTGTGTGTCTCTGTGCCCTGCAGTGTCCTTTACCCCCATTACAACTGGACTGCCTCTAGCCCTGTTTA
  TGGATCCTGGTTCAAGGAAGGGGCCGATATACCATGGGATATTCCAGTGGCCACAAACACCCCAAGTGGA
  AAAGTGCAAGAGGATACCCACGGTCGATTCCTCCTCCTTGGGGACCCACAGACCAACAACTGCTCCCTGA
  GCATCAGAGATGCCAGGAAGGGGGATTCAGGGAAGTACTACTTCCAGGTGGAGAGAGGAAGCAGGAAATG
  GAACTACATATATGACAAGCTCTCTGTGCATGTGACAGCCCTGACTCACATGCCCACCTTCTCCATCCCG
  GGGACCCTGGAGTCTGGCCACCCCAGGAACCTGACCTGCTCTGTGCCCTGGGCCTGTGAACAGGGGACGC
  CCCCCACGATCACCTGGATGGGGGCCTCCGTGTCCTCCCTGGACCCCACTATCACTCGCTCCTCGATGCT
  CAGCCTCATCCCACAGCCCCAGGACCATGGCACCAGCCTCACCTGTCAGGTGACCTTGCCTGGGGCCGGC
  GTGACCATGACCAGGGCTGTCCGACTCAACATATCCTATCCTCCTCAGAACTTGACCATGACTGTCTTCC
  AAGGAGATGGCACAGCATCCACAACCTTGAGGAATGGCTCGGCCCTTTCAGTCCTGGAGGGCCAGTCCCT
  GCACCTTGTCTGTGCTGTCGACAGCAATCCCCCTGCCAGGCTGAGCTGGACCTGGGGGAGCCTGACCCTG
  AGCCCCTCACAGTCCTCGAACCTTGGGGTGCTGGAGCTGCCTCGAGTGCATGTGAAGGATGAAGGGGAAT
  TCACCTGCCGAGCTCAGAACCCTCTAGGCTCCCAGCACATTTCCCTGAGCCTCTCCCTGCAAAACGAGTA
  CACAGGCAAAATGAGGCCTATATCAGGAGTGACGCTAGGGGCATTCGGGGGAGCTGGAGCCACAGCCCTG
  GTCTTCCTGTACTTCTGCATCATCTTCGTTGTAGTGAGGTCCTGCAGGAAGAAATCGGCAAGGCCAGCAG
  TGGGCGTGGGGGATACAGGCATGGAGGACGCAAACGCTGTCAGGGGCTCAGCCTCTCAGGGACCCCTGAT
  TGAATCCCCGGCAGATGACAGCCCCCCACACCATGCTCCGCCAGCCCTGGCCACCCCCTCCCCAGAGGAA
  GGAGAGATCCAGTATGCATCCCTCAGCTTCCACAAAGCGAGGCCTCAGTACCCACAGGAACAGGAGGCCA
  TCGGCTATGAGTACTCCGAGATCAACATCCCCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207808 representing NM_053003
  Red=Cloning site Green=Tags(s)
  
  MLLLLLLLPPLLCGRVGAKEQKDYLLTMQKSVTVQEGLCVSVLCSFSYPQNGWTASDPVHGYWFRAGDHV
  SRNIPVATNNPARAVQEETRDRFHLLGDPQNKDCTLSIRDTRESDAGTYVFCVERGNMKWNYKYDQLSVN
  VTASQDLLSRYRLEVPESVTVQEGLCVSVPCSVLYPHYNWTASSPVYGSWFKEGADIPWDIPVATNTPSG
  KVQEDTHGRFLLLGDPQTNNCSLSIRDARKGDSGKYYFQVERGSRKWNYIYDKLSVHVTALTHMPTFSIP
  GTLESGHPRNLTCSVPWACEQGTPPTITWMGASVSSLDPTITRSSMLSLIPQPQDHGTSLTCQVTLPGAG
  VTMTRAVRLNISYPPQNLTMTVFQGDGTASTTLRNGSALSVLEGQSLHLVCAVDSNPPARLSWTWGSLTL
  SPSQSSNLGVLELPRVHVKDEGEFTCRAQNPLGSQHISLSLSLQNEYTGKMRPISGVTLGAFGGAGATAL
  VFLYFCIIFVVVRSCRKKSARPAVGVGDTGMEDANAVRGSASQGPLIESPADDSPPHHAPPALATPSPEE
  GEIQYASLSFHKARPQYPQEQEAIGYEYSEINIPK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053003
• ORF Size: 1785 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053003.2, NP_443729.1
• RefSeq Size: 2126
• RefSeq ORF: 1788
• Locus ID: 89858
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane
• Gene Summary: Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]