WRCH1 (RHOU) (NM_021205) Human Tagged ORF Clone

CAT#: RG207871

RHOU (GFP-tagged) - Human ras homolog gene family, member U (RHOU), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RHOU
• Synonyms: ARHU; CDC42L1; G28K; hG28K; WRCH1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207871 representing NM_021205
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCGCAGCAGGGGGACCCCGCGTTCCCCGACCGCTGCGAGGCGCCTCCGGTGCCGCCGCGTCGGG
  AGCGCGGTGGACGCGGGGGACGCGGGCCTGGGGAGCCGGGGGGCCGGGGGCGTGCGGGGGGTGCCGAGGG
  GCGCGGCGTCAAGTGCGTGCTGGTCGGCGACGGCGCGGTGGGCAAGACGAGCCTGGTGGTGAGCTACACC
  ACCAACGGCTACCCCACCGAGTACATCCCTACTGCCTTCGACAACTTCTCCGCGGTGGTGTCTGTGGATG
  GGCGGCCCGTGAGACTCCAACTCTGTGACACTGCCGGACAGGATGAATTTGACAAGCTGAGGCCTCTCTG
  CTACACCAACACAGACATCTTCCTGCTCTGCTTCAGTGTCGTGAGCCCCTCATCCTTCCAGAACGTCAGT
  GAGAAATGGGTGCCGGAGATTCGATGCCACTGTCCCAAAGCCCCCATCATCCTAGTTGGAACGCAGTCGG
  ATCTCAGAGAAGATGTCAAAGTCCTCATTGAGTTGGACAAATGCAAAGAAAAGCCAGTGCCTGAAGAGGC
  GGCTAAGCTGTGCGCCGAGGAAATCAAAGCCGCCTCCTACATCGAGTGTTCAGCCTTGACTCAAAAAAAC
  CTCAAAGAGGTCTTTGATGCAGCCATCGTCGCTGGCATTCAATACTCGGACACTCAGCAACAGCCAAAGA
  AGTCTAAAAGCAGGACTCCAGATAAAATGAAAAACCTCTCCAAGTCCTGGTGGAAGAAGTACTGCTGTTT
  CGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207871 representing NM_021205
  Red=Cloning site Green=Tags(s)
  
  MPPQQGDPAFPDRCEAPPVPPRRERGGRGGRGPGEPGGRGRAGGAEGRGVKCVLVGDGAVGKTSLVVSYT
  TNGYPTEYIPTAFDNFSAVVSVDGRPVRLQLCDTAGQDEFDKLRPLCYTNTDIFLLCFSVVSPSSFQNVS
  EKWVPEIRCHCPKAPIILVGTQSDLREDVKVLIELDKCKEKPVPEEAAKLCAEEIKAASYIECSALTQKN
  LKEVFDAAIVAGIQYSDTQQQPKKSKSRTPDKMKNLSKSWWKKYCCFV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021205
• ORF Size: 774 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021205.4, NP_067028.1
• RefSeq Size: 4369
• RefSeq ORF: 777
• Locus ID: 58480
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus. [provided by RefSeq, Mar 2011]