CROT (NM_021151) Human Tagged ORF Clone

CAT#: RG207888

CROT (GFP-tagged) - Human carnitine O-octanoyltransferase (CROT), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CROT
• Synonyms: COT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207888 representing NM_021151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATCAATTGGCTAAATCAACTGAAGAACGAACATTTCAGTACCAGGATTCTCTTCCATCACTGC
  CTGTTCCTTCACTTGAAGAATCATTAAAAAAATACCTTGAATCAGTGAAACCATTTGCAAATCAAGAAGA
  ATATAAGAAAACTGAAGAAATAGTTCAAAAATTTCAAAGTGGGATTGGAGAAAAATTGCACCAGAAATTG
  CTTGAAAGAGCAAAAGGAAAAAGAAATTGGCTGGAAGAGTGGTGGCTGAATGTTGCCTATCTGGATGTTC
  GTATACCATCACAATTGAATGTCAACTTTGCGGGTCCTGCAGCTCATTTTGAACACTACTGGCCTCCAAA
  GGAAGGGACTCAATTAGAAAGAGGAAGTATAACTCTTTGGCATAACTTGAACTACTGGCAGCTATTAAGA
  AAAGAAAAAGTGCCTGTTCATAAAGTTGGAAATACTCCTCTAGATATGAATCAATTCCGAATGCTATTTT
  CTACCTGCAAGGTTCCAGGAATTACTAGAGACTCCATTATGAATTATTTTAGGACTGAGAGTGAAGGGCG
  TTCCCCAAACCACATTGTAGTGCTGTGTCGAGGCCGAGCTTTTGTCTTTGATGTAATACATGAAGGATGT
  TTGGTCACCCCGCCAGAGCTTCTCAGACAACTGACATATATCCACAAGAAGTGCCATAGTGAACCTGATG
  GACCTGGGATTGCAGCATTAACTAGTGAGGAGCGAACTCGATGGGCTAAGGCACGAGAATATCTGATTGG
  TCTTGATCCAGAGAACTTGGCTTTGTTAGAAAAAATTCAGAGTAGTTTACTGGTATATTCCATGGAGGAT
  AGCAGTCCACATGTAACACCAGAGGATTATTCTGAGATTATTGCAGCCATCCTTATTGGAGATCCAACAG
  TACGCTGGGGTGACAAATCCTATAACTTGATTTCCTTTTCTAATGGAGTATTTGGCTGTAATTGTGATCA
  TGCTCCTTTTGATGCAATGATTATGGTGAACATCAGTTATTATGTGGATGAGAAAATTTTTCAGAATGAA
  GGAAGATGGAAGGGTTCAGAGAAGGTACGAGATATACCACTTCCAGAAGAGCTCATTTTCATTGTGGATG
  AGAAAGTTTTAAATGACATCAACCAAGCTAAAGCCCAGTATCTCAGGGAGGCATCTGATCTACAGATTGC
  GGCTTATGCCTTTACATCTTTTGGCAAAAAGCTAACCAAGAACAAGATGCTTCACCCGGATACGTTTATT
  CAGCTTGCACTTCAGCTGGCCTATTACAGACTTCATGGACACCCTGGTTGTTGCTATGAAACAGCTATGA
  CAAGACATTTTTATCATGGCCGTACAGAGACTATGCGATCATGCACAGTTGAAGCAGTGAGGTGGTGCCA
  GTCCATGCAGGATCCTTCTGTCAATCTTCGTGAGCGGCAGCAAAAGATGTTACAAGCTTTTGCAAAGCAT
  AATAAAATGATGAAAGATTGTTCAGCTGGAAAAGGATTTGATCGTCACCTTTTAGGTCTCTTACTCATAG
  CAAAAGAGGAAGGTCTTCCTGTTCCAGAACTCTTTACGGACCCACTTTTTTCCAAAAGCGGAGGAGGTGG
  AAATTTTGTTCTCTCAACAAGTCTGGTTGGCTATTTACGAGTCCAGGGAGTGGTAGTTCCCATGGTACAC
  AATGGTTATGGATTTTTCTACCATATCAGAGATGACAGGTTTGTTGTGGCCTGTTCAGCCTGGAAATCCT
  GTCCCGAGACTGATGCGGAAAAGCTAGTTCAGCTGACTTTTTGTGCTTTTCATGATATGATACAGCTGAT
  GAACTCTACTCATCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207888 representing NM_021151
  Red=Cloning site Green=Tags(s)
  
  MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVKPFANQEEYKKTEEIVQKFQSGIGEKLHQKL
  LERAKGKRNWLEEWWLNVAYLDVRIPSQLNVNFAGPAAHFEHYWPPKEGTQLERGSITLWHNLNYWQLLR
  KEKVPVHKVGNTPLDMNQFRMLFSTCKVPGITRDSIMNYFRTESEGRSPNHIVVLCRGRAFVFDVIHEGC
  LVTPPELLRQLTYIHKKCHSEPDGPGIAALTSEERTRWAKAREYLIGLDPENLALLEKIQSSLLVYSMED
  SSPHVTPEDYSEIIAAILIGDPTVRWGDKSYNLISFSNGVFGCNCDHAPFDAMIMVNISYYVDEKIFQNE
  GRWKGSEKVRDIPLPEELIFIVDEKVLNDINQAKAQYLREASDLQIAAYAFTSFGKKLTKNKMLHPDTFI
  QLALQLAYYRLHGHPGCCYETAMTRHFYHGRTETMRSCTVEAVRWCQSMQDPSVNLRERQQKMLQAFAKH
  NKMMKDCSAGKGFDRHLLGLLLIAKEEGLPVPELFTDPLFSKSGGGGNFVLSTSLVGYLRVQGVVVPMVH
  NGYGFFYHIRDDRFVVACSAWKSCPETDAEKLVQLTFCAFHDMIQLMNSTHL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021151
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021151.2, NP_066974.2
• RefSeq Size: 3211
• RefSeq ORF: 1839
• Locus ID: 54677
• Domains: Carn_acyltransf
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]