KAT2A (NM_021078) Human Tagged ORF Clone

CAT#: RG207889

KAT2A (GFP-tagged) - Human K(lysine) acetyltransferase 2A (KAT2A)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KAT2A
• Synonyms: GCN5; GCN5L2; hGCN5; PCAF-b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207889 representing NM_021078
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAACCTTCCCAGGCCCCGACCCCGGCCCCGGCTGCGCAGCCCCGGCCCCTTCAGTCCCCAGCCC
  CTGCCCCAACTCCGACTCCTGCACCCAGCCCGGCTTCAGCCCCGATTCCGACTCCCACCCCGGCACCAGC
  CCCTGCCCCAGCTGCAGCCCCAGCCGGCAGCACAGGGACTGGGGGGCCCGGGGTAGGAAGTGGGGGGGCC
  GGGAGCGGGGGGGATCCGGCTCGGCCTGGCCTGAGCCAGCAGCAGCGCGCCAGTCAGAGGAAGGCGCAAG
  TCCGGGGGCTGCCCCGCGCCAAGAAGCTTGAGAAGCTAGGGGTCTTCTCGGCTTGCAAGGCCAATGGAAC
  CTGTAAGTGTAATGGCTGGAAAAACCCCAAGCCCCCCACTGCACCCCGCATAGATCTGCAGCAGCCAGCT
  GCCAACCTGAGTGAGCTGTGCCGCAGTTGTGAGCACCCCTTGGCTGACCACGTATCCCACTTGGAGAATG
  TGTCAGAGGATGAGATAAACCGACTGCTGGGGATGGTGGTGGATGTGGAGAATCTCTTCATGTCTGTTCA
  CAAGGAAGAGGACACAGACACCAAGCAGGTCTATTTCTACCTCTTCAAGCTACTGCGGAAATGCATCCTG
  CAGATGACCCGGCCTGTGGTGGAGGGGTCCCTGGGCAGCCCTCCATTTGAGAAACCTAATATTGAGCAGG
  GTGTGCTGAACTTTGTGCAGTACAAGTTTAGTCACCTGGCTCCCCGGGAGCGGCAGACGATGTTCGAGCT
  CTCAAAGATGTTCTTGCTCTGCCTTAACTACTGGGAGCTTGAGACACCTGCCCAGTTTCGGCAGAGGTCT
  CAGGCTGAGGACGTGGCTACCTACAAGGTCAATTACACCAGATGGCTCTGTTACTGCCACGTGCCCCAGA
  GCTGTGATAGCCTCCCCCGCTACGAAACCACTCATGTCTTTGGGCGAAGCCTTCTCCGGTCCATTTTCAC
  CGTTACCCGCCGGCAGCTGCTGGAAAAGTTCCGAGTGGAGAAGGACAAATTGGTGCCCGAGAAGAGGACC
  CTCATCCTCACTCACTTCCCCAAATTCCTGTCCATGCTGGAGGAGGAGATCTATGGGGCAAACTCTCCAA
  TCTGGGAGTCAGGCTTCACCATGCCACCCTCAGAGGGGACACAGCTGGTTCCCCGGCCAGCTTCAGTCAG
  TGCAGCGGTTGTTCCCAGCACCCCCATCTTCAGCCCCAGCATGGGTGGGGGCAGCAACAGCTCCCTGAGT
  CTGGATTCTGCAGGGGCCGAGCCTATGCCAGGCGAGAAGAGGACGCTCCCAGAGAACCTGACCCTGGAGG
  ATGCCAAGCGGCTCCGTGTGATGGGTGACATCCCCATGGAGCTGGTCAATGAGGTCATGCTGACCATCAC
  TGACCCTGCTGCCATGCTGGGGCCTGAGACGAGCCTGCTTTCGGCCAATGCGGCCCGGGATGAGACAGCC
  CGCCTGGAGGAGCGCCGCGGCATCATCGAGTTCCATGTCATCGGCAACTCACTGACGCCCAAGGCCAACC
  GGCGGGTGTTGCTGTGGCTCGTGGGGCTGCAGAATGTCTTTTCCCACCAGCTGCCGCGCATGCCTAAGGA
  GTATATCGCCCGCCTCGTCTTTGACCCGAAGCACAAGACTCTGGCCTTGATCAAGGATGGGCGGGTCATC
  GGTGGCATCTGCTTCCGCATGTTTCCCACCCAGGGCTTCACGGAGATTGTCTTCTGTGCTGTCACCTCGA
  ATGAGCAGGTCAAGGGTTATGGGACCCACCTGATGAACCACCTGAAGGAGTATCACATCAAGCACAACAT
  TCTCTACTTCCTCACCTACGCCGACGAGTACGCCATCGGCTACTTCAAAAAGCAGGGTTTCTCCAAGGAC
  ATCAAGGTGCCCAAGAGCCGCTACCTGGGCTACATCAAGGACTACGAGGGAGCGACGCTGATGGAGTGTG
  AGCTGAATCCCCGCATCCCCTACACGGAGCTGTCCCACATCATCAAGAAGCAGAAAGAGATCATCAAGAA
  GCTGATTGAGCGCAAACAGGCCCAGATCCGCAAGGTCTACCCGGGGCTCAGCTGCTTCAAGGAGGGCGTG
  AGGCAGATCCCTGTGGAGAGCGTTCCTGGCATTCGAGAGACAGGCTGGAAGCCATTGGGGAAGGAGAAGG
  GGAAGGAGCTGAAGGACCCCGACCAGCTCTACACAACCCTCAAAAACCTGCTGGCCCAAATCAAGTCTCA
  CCCCAGTGCCTGGCCCTTCATGGAGCCTGTGAAGAAGTCGGAGGCCCCTGACTACTACGAGGTCATCCGC
  TTCCCCATTGACCTGAAGACCATGACTGAGCGGCTGCGAAGCCGCTACTACGTGACCCGGAAGCTCTTTG
  TGGCCGACCTGCAGCGGGTCATCGCCAACTGTCGCGAGTACAACCCCCCGGACAGCGAGTACTGCCGCTG
  TGCCAGCGCCCTGGAGAAGTTCTTCTACTTCAAGCTCAAGGAGGGAGGCCTCATTGACAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207889 representing NM_021078
  Red=Cloning site Green=Tags(s)
  
  MAEPSQAPTPAPAAQPRPLQSPAPAPTPTPAPSPASAPIPTPTPAPAPAPAAAPAGSTGTGGPGVGSGGA
  GSGGDPARPGLSQQQRASQRKAQVRGLPRAKKLEKLGVFSACKANGTCKCNGWKNPKPPTAPRIDLQQPA
  ANLSELCRSCEHPLADHVSHLENVSEDEINRLLGMVVDVENLFMSVHKEEDTDTKQVYFYLFKLLRKCIL
  QMTRPVVEGSLGSPPFEKPNIEQGVLNFVQYKFSHLAPRERQTMFELSKMFLLCLNYWELETPAQFRQRS
  QAEDVATYKVNYTRWLCYCHVPQSCDSLPRYETTHVFGRSLLRSIFTVTRRQLLEKFRVEKDKLVPEKRT
  LILTHFPKFLSMLEEEIYGANSPIWESGFTMPPSEGTQLVPRPASVSAAVVPSTPIFSPSMGGGSNSSLS
  LDSAGAEPMPGEKRTLPENLTLEDAKRLRVMGDIPMELVNEVMLTITDPAAMLGPETSLLSANAARDETA
  RLEERRGIIEFHVIGNSLTPKANRRVLLWLVGLQNVFSHQLPRMPKEYIARLVFDPKHKTLALIKDGRVI
  GGICFRMFPTQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHNILYFLTYADEYAIGYFKKQGFSKD
  IKVPKSRYLGYIKDYEGATLMECELNPRIPYTELSHIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKEGV
  RQIPVESVPGIRETGWKPLGKEKGKELKDPDQLYTTLKNLLAQIKSHPSAWPFMEPVKKSEAPDYYEVIR
  FPIDLKTMTERLRSRYYVTRKLFVADLQRVIANCREYNPPDSEYCRCASALEKFFYFKLKEGGLIDK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021078
• ORF Size: 2511 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021078.1, NP_066564.1
• RefSeq Size: 3074 bp
• RefSeq ORF: 2514 bp
• Locus ID: 2648
• Cytogenetics: 17q21.2
• Protein Families: Transcription Factors
• Protein Pathways: Notch signaling pathway
• Gene Summary: 'KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009]'