PTP epsilon (PTPRE) (NM_006504) Human Tagged ORF Clone

CAT#: RG207950

PTPRE (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, E (PTPRE), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRE
• Synonyms: HPTPE; PTPE; R-PTP-EPSILON
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207950 representing NM_006504
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCTTGTGTCCACTCCTGCTGGTGGGTTTTAGCTTGCCGCTCGCCAGGGCTCTCAGGGGCAACG
  AGACCACTGCCGACAGCAACGAGACAACCACGACCTCAGGCCCTCCGGACCCGGGCGCCTCCCAGCCGCT
  GCTGGCCTGGCTGCTACTGCCGCTGCTGCTCCTCCTCCTCGTGCTCCTTCTCGCCGCCTACTTCTTCAGG
  TTCAGGAAGCAGAGGAAAGCTGTGGTCAGCACCAGCGACAAGAAGATGCCCAACGGAATCTTGGAGGAGC
  AAGAGCAGCAAAGGGTGATGCTGCTCAGCAGGTCACCCTCAGGGCCCAAGAAGTATTTTCCCATCCCCGT
  GGAGCACCTGGAGGAGGAGATCCGTATCAGATCCGCCGACGACTGCAAGCAGTTTCGGGAGGAGTTCAAC
  TCATTGCCATCTGGACACATACAAGGAACTTTTGAACTGGCAAATAAAGAAGAAAACAGAGAAAAAAACA
  GATATCCCAACATCCTTCCCAATGACCATTCTAGGGTGATTCTGAGCCAACTGGATGGAATTCCCTGTTC
  AGACTACATCAATGCTTCCTACATAGATGGTTACAAAGAGAAGAATAAATTCATAGCAGCTCAAGGTCCC
  AAACAGGAAACGGTTAACGACTTCTGGAGAATGGTCTGGGAGCAAAAGTCTGCGACCATCGTCATGTTAA
  CAAACTTGAAAGAAAGGAAAGAGGAAAAGTGCCATCAGTACTGGCCCGACCAAGGCTGCTGGACCTATGG
  AAACATCCGGGTGTGCGTGGAGGACTGCGTGGTTTTGGTCGACTACACCATCCGGAAGTTCTGCATACAG
  CCACAGCTCCCCGACGGCTGCAAAGCCCCCAGGCTGGTCTCACAGCTGCACTTCACCAGCTGGCCCGACT
  TCGGAGTGCCTTTTACCCCCATTGGGATGCTGAAGTTCCTCAAGAAAGTAAAGACGCTCAACCCCGTGCA
  CGCTGGGCCCATCGTGGTCCACTGTAGCGCGGGCGTGGGCCGGACGGGCACCTTCATTGTGATCGATGCC
  ATGATGGCCATGATGCACGCGGAGCAGAAGGTGGATGTGTTTGAATTTGTGTCTCGAATCCGTAATCAGC
  GCCCTCAGATGGTTCAAACGGATATGCAGTACACGTTCATCTACCAAGCCTTACTCGAGTACTACCTCTA
  CGGGGACACAGAGCTGGACGTGTCCTCACTGGAGAAGCACCTGCAGACCATGCACGGCACCACAACCCAC
  TTCGACAAGATCGGGCTGGAGGAGGAGTTCAGGAAATTGACAAATGTCCGGATCATGAAGGAGAACATGA
  GGACGGGCAACTTGCCGGCAAACATGAAGAAGGCCAGGGTCATCCAGATCATCCCGTATGACTTCAACCG
  AGTGATCCTTTCCATGAAAAGGGGTCAAGAATACACAGACTACATCAACGCATCCTTCATAGACGGCTAC
  CGACAGAAGGACTATTTCATCGCCACCCAGGGGCCACTGGCACACACGGTTGAGGACTTCTGGAGGATGA
  TCTGGGAATGGAAATCCCACACTATCGTGATGCTGACGGAGGTGCAGGAGAGAGAGCAGGATAAATGCTA
  CCAGTATTGGCCAACCGAGGGCTCAGTTACTCATGGAGAAATAACGATTGAGATAAAGAATGATACCCTT
  TCAGAAGCCATCAGTATACGAGACTTTCTGGTCACTCTCAATCAGCCCCAGGCCCGCCAGGAGGAGCAGG
  TCCGAGTAGTGCGCCAGTTTCACTTCCACGGCTGGCCTGAGATCGGGATTCCCGCCGAGGGCAAAGGCAT
  GATTGACCTCATCGCAGCCGTGCAGAAGCAGCAGCAGCAGACAGGCAACCACCCCATCACCGTGCACTGC
  AGTGCCGGAGCTGGGCGAACAGGTACATTCATAGCCCTCAGCAACATTTTGGAGCGAGTAAAAGCCGAGG
  GGCTTTTAGATGTATTTCAAGCTGTGAAGAGTTTACGACTTCAGAGACCACATATGGTGCAAACCCTGGA
  ACAGTATGAATTCTGCTACAAAGTGGTACAAGATTTTATTGATATATTTTCTGATTATGCTAATTTCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207950 representing NM_006504
  Red=Cloning site Green=Tags(s)
  
  MEPLCPLLLVGFSLPLARALRGNETTADSNETTTTSGPPDPGASQPLLAWLLLPLLLLLLVLLLAAYFFR
  FRKQRKAVVSTSDKKMPNGILEEQEQQRVMLLSRSPSGPKKYFPIPVEHLEEEIRIRSADDCKQFREEFN
  SLPSGHIQGTFELANKEENREKNRYPNILPNDHSRVILSQLDGIPCSDYINASYIDGYKEKNKFIAAQGP
  KQETVNDFWRMVWEQKSATIVMLTNLKERKEEKCHQYWPDQGCWTYGNIRVCVEDCVVLVDYTIRKFCIQ
  PQLPDGCKAPRLVSQLHFTSWPDFGVPFTPIGMLKFLKKVKTLNPVHAGPIVVHCSAGVGRTGTFIVIDA
  MMAMMHAEQKVDVFEFVSRIRNQRPQMVQTDMQYTFIYQALLEYYLYGDTELDVSSLEKHLQTMHGTTTH
  FDKIGLEEEFRKLTNVRIMKENMRTGNLPANMKKARVIQIIPYDFNRVILSMKRGQEYTDYINASFIDGY
  RQKDYFIATQGPLAHTVEDFWRMIWEWKSHTIVMLTEVQEREQDKCYQYWPTEGSVTHGEITIEIKNDTL
  SEAISIRDFLVTLNQPQARQEEQVRVVRQFHFHGWPEIGIPAEGKGMIDLIAAVQKQQQQTGNHPITVHC
  SAGAGRTGTFIALSNILERVKAEGLLDVFQAVKSLRLQRPHMVQTLEQYEFCYKVVQDFIDIFSDYANFK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006504
• ORF Size: 2100 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006504.3, NP_006495.1
• RefSeq Size: 5376 bp
• RefSeq ORF: 2103 bp
• Locus ID: 5791
• Cytogenetics: 10q26.2
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Several alternatively spliced transcript variants of this gene have been reported, at least two of which encode a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains; another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokine-induced SATA signaling, as well as the activation of voltage-gated K+ channels. [provided by RefSeq, Oct 2015]'