GNAL (NM_182978) Human Tagged ORF Clone

CAT#: RG208226

GNAL (GFP-tagged) - Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type (GNAL), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GNAL
• Synonyms: DYT25
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208226 representing NM_182978
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTGTGCTACAGTCTGCGGCCGCTGCTTTTCGGGGGCCCAGGGGACGACCCCTGCGCGGCCTCGG
  AGCCGCCGGTGGAGGACGCGCAGCCCGCCCCGGCCCCGGCCCTGGCCCCAGTCCGGGCGGCCGCAAGGGA
  CACGGCCCGGACCCTGCTCCCTCGGGGCGGCGAAGGGAGCCCGGCATGCGCTCGGCCCAAAGCAGACAAG
  CCGAAGGAGAAGCGGCAGCGCACCGAGCAGCTTAGTGCCGAGGAGCGCGAGGCGGCCAAGGAGCGCGAGG
  CGGTCAAGGAGGCGAGGAAAGTGAGCCGGGGCATCGACCGCATGCTGCGCGACCAGAAGCGCGACCTGCA
  GCAGACGCACCGGCTCCTGCTGCTCGGGGCTGGTGAGTCTGGGAAAAGCACTATCGTCAAACAGATGAGG
  ATCCTGCACGTCAATGGGTTTAATCCCGAGGAAAAGAAACAGAAAATTCTGGACATCCGGAAAAATGTTA
  AAGATGCTATCGTGACAATTGTTTCAGCAATGAGTACTATAATACCTCCAGTTCCGCTGGCCAACCCTGA
  AAACCAATTTCGATCAGACTACATCAAGAGCATAGCCCCTATCACTGACTTTGAATATTCCCAGGAATTC
  TTTGACCATGTGAAAAAACTTTGGGACGATGAAGGCGTGAAGGCATGCTTTGAGAGATCCAACGAATACC
  AGCTGATTGACTGTGCACAATACTTCCTGGAAAGAATCGACAGCGTCAGCTTGGTTGACTACACACCCAC
  AGACCAGGACCTCCTCAGATGCAGAGTTCTGACATCTGGGATTTTTGAGACACGATTCCAAGTGGACAAA
  GTAAACTTCCACATGTTTGATGTTGGTGGCCAGAGGGATGAGAGGAGAAAATGGATCCAGTGCTTTAACG
  ATGTCACAGCTATCATTTACGTCGCAGCCTGCAGTAGCTACAACATGGTGATTCGAGAAGATAACAACAC
  CAACAGGCTGAGAGAGTCCCTGGATCTTTTTGAAAGCATCTGGAACAACAGGTGGTTACGGACCATTTCT
  ATCATCTTGTTCTTGAACAAACAAGATATGCTGGCAGAAAAAGTCTTGGCAGGGAAATCAAAAATTGAAG
  ACTATTTCCCAGAATATGCAAATTATACTGTTCCTGAAGACGCAACACCAGATGCAGGAGAAGATCCCAA
  AGTTACAAGAGCCAAGTTCTTTATCCGGGACCTGTTTTTGAGGATCAGCACGGCCACCGGTGACGGCAAA
  CATTACTGCTACCCGCACTTCACCTGCGCCGTGGACACAGAGAACATCCGCAGGGTGTTCAACGACTGCC
  GCGACATCATCCAGCGGATGCACCTCAAGCAGTATGAGCTCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208226 representing NM_182978
  Red=Cloning site Green=Tags(s)
  
  MGLCYSLRPLLFGGPGDDPCAASEPPVEDAQPAPAPALAPVRAAARDTARTLLPRGGEGSPACARPKADK
  PKEKRQRTEQLSAEEREAAKEREAVKEARKVSRGIDRMLRDQKRDLQQTHRLLLLGAGESGKSTIVKQMR
  ILHVNGFNPEEKKQKILDIRKNVKDAIVTIVSAMSTIIPPVPLANPENQFRSDYIKSIAPITDFEYSQEF
  FDHVKKLWDDEGVKACFERSNEYQLIDCAQYFLERIDSVSLVDYTPTDQDLLRCRVLTSGIFETRFQVDK
  VNFHMFDVGGQRDERRKWIQCFNDVTAIIYVAACSSYNMVIREDNNTNRLRESLDLFESIWNNRWLRTIS
  IILFLNKQDMLAEKVLAGKSKIEDYFPEYANYTVPEDATPDAGEDPKVTRAKFFIRDLFLRISTATGDGK
  HYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLKQYELL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182978
• ORF Size: 1374 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182978.1, NP_892023.1
• RefSeq Size: 6491 bp
• RefSeq ORF: 1377 bp
• Locus ID: 2774
• Cytogenetics: 18p11.21
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Olfactory transduction
• Gene Summary: 'This gene encodes a stimulatory G protein alpha subunit which mediates odorant signaling in the olfactory epithelium. This protein couples dopamine type 1 receptors and adenosine A2A receptors and is widely expressed in the central nervous system. Mutations in this gene have been associated with dystonia 25 and this gene is located in a susceptibility region for bipolar disorder and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]'