MMP19 (NM_002429) Human Tagged ORF Clone

CAT#: RG208245

  • TrueORF®

MMP19 (GFP-tagged) - Human matrix metallopeptidase 19 (MMP19), transcript variant 1


  "NM_002429" in other vectors (4)

Reconstitution Protocol

USD 460.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol MMP19
Synonyms CODA; MMP18; RASI-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG208245 representing NM_002429
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTGCCAGCAGCTGTGGCTGGGCTTCCTACTCCCCATGACAGTCTCAGGCCGGGTCCTGGGGCTTG
CAGAGGTGGCGCCCGTGGACTACCTGTCACAATATGGGTACCTACAGAAGCCTCTAGAAGGATCTAATAA
CTTCAAGCCAGAAGATATCACCGAGGCTCTGAGAGCTTTTCAGGAAGCATCTGAACTTCCAGTCTCAGGT
CAGCTGGATGATGCCACAAGGGCCCGCATGAGGCAGCCTCGTTGTGGCCTAGAGGATCCCTTCAACCAGA
AGACCCTTAAATACCTGTTGCTGGGCCGCTGGAGAAAGAAGCACCTGACTTTCCGCATCTTGAACCTGCC
CTCCACCCTTCCACCCCACACAGCCCGGGCAGCCCTGCGTCAAGCCTTCCAGGACTGGAGCAATGTGGCT
CCCTTGACCTTCCAAGAGGTGCAGGCTGGTGCGGCTGACATCCGCCTCTCCTTCCATGGCCGCCAAAGCT
CGTACTGTTCCAATACTTTTGATGGGCCTGGGAGAGTCCTGGCCCATGCCGACATCCCAGAGCTGGGCAG
TGTGCACTTCGACGAAGACGAGTTCTGGACTGAGGGGACCTACCGTGGGGTGAACCTGCGCATCATTGCA
GCCCATGAAGTGGGCCATGCTCTGGGGCTTGGGCACTCCCGATATTCCCAGGCCCTCATGGCCCCAGTCT
ACGAGGGCTACCGGCCCCACTTTAAGCTGCACCCAGATGATGTGGCAGGGATCCAGGCTCTCTATGGCAA
GAAGAGTCCAGTGATAAGGGATGAGGAAGAAGAAGAGACAGAGCTGCCCACTGTGCCCCCAGTGCCCACA
GAACCCAGTCCCATGCCAGACCCTTGCAGTAGTGAACTGGATGCCATGATGCTGGGGCCCCGTGGGAAGA
CCTATGCTTTCAAGGGGGACTATGTGTGGACTGTATCAGATTCAGGACCGGGCCCCTTGTTCCGAGTGTC
TGCCCTTTGGGAGGGGCTCCCCGGAAACCTGGATGCTGCTGTCTACTCGCCTCGAACACAATGGATTCAC
TTCTTTAAGGGAGACAAGGTGTGGCGCTACATTAATTTCAAGATGTCTCCTGGCTTCCCCAAGAAGCTGA
ATAGGGTAGAACCTAACCTGGATGCAGCTCTCTATTGGCCTCTCAACCAAAAGGTGTTCCTCTTTAAGGG
CTCCGGGTACTGGCAGTGGGACGAGCTAGCCCGAACTGACTTCAGCAGCTACCCCAAACCAATCAAGGGT
TTGTTTACGGGAGTGCCAAACCAGCCCTCGGCTGCTATGAGTTGGCAAGATGGCCGAGTCTACTTCTTCA
AGGGCAAAGTCTACTGGCGCCTCAACCAGCAGCTTCGAGTAGAGAAAGGCTATCCCAGAAATATTTCCCA
CAACTGGATGCACTGTCGTCCCCGGACTATAGACACTACCCCATCAGGTGGGAATACCACTCCCTCAGGT
ACGGGCATAACCTTGGATACCACTCTCTCAGCCACAGAAACCACGTTTGAATAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG208245 representing NM_002429
Red=Cloning site Green=Tags(s)

MNCQQLWLGFLLPMTVSGRVLGLAEVAPVDYLSQYGYLQKPLEGSNNFKPEDITEALRAFQEASELPVSG
QLDDATRARMRQPRCGLEDPFNQKTLKYLLLGRWRKKHLTFRILNLPSTLPPHTARAALRQAFQDWSNVA
PLTFQEVQAGAADIRLSFHGRQSSYCSNTFDGPGRVLAHADIPELGSVHFDEDEFWTEGTYRGVNLRIIA
AHEVGHALGLGHSRYSQALMAPVYEGYRPHFKLHPDDVAGIQALYGKKSPVIRDEEEEETELPTVPPVPT
EPSPMPDPCSSELDAMMLGPRGKTYAFKGDYVWTVSDSGPGPLFRVSALWEGLPGNLDAAVYSPRTQWIH
FFKGDKVWRYINFKMSPGFPKKLNRVEPNLDAALYWPLNQKVFLFKGSGYWQWDELARTDFSSYPKPIKG
LFTGVPNQPSAAMSWQDGRVYFFKGKVYWRLNQQLRVEKGYPRNISHNWMHCRPRTIDTTPSGGNTTPSG
TGITLDTTLSATETTFEY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002429
ORF Size 1524 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002429.4, NP_002420.1
RefSeq Size 3262 bp
RefSeq ORF 1527 bp
Locus ID 4327
Cytogenetics 12q13.2
Domains hemopexin, Peptidase_M10
Protein Families Protease, Secreted Protein
Gene Summary 'This gene encodes a member of a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded protein is secreted as an inactive proprotein, which is activated upon cleavage by extracellular proteases. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013]'

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