PLCD1 (NM_006225) Human Tagged ORF Clone

CAT#: RG208263

PLCD1 (GFP-tagged) - Human phospholipase C, delta 1 (PLCD1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PLCD1
• Synonyms: NDNC3; PLC-III
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208263 representing NM_006225
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGGGCCGGGACTTCCTGACCCTGCACGGCCTACAGGATGATGAGGATCTACAGGCGCTGCTGA
  AGGGCAGCCAGCTCCTGAAGGTGAAGTCCAGCTCATGGAGGAGAGAGCGCTTCTACAAGTTGCAGGAGGA
  CTGCAAGACCATCTGGCAGGAGTCCCGCAAGGTCATGCGGACCCCGGAGTCCCAGCTGTTCTCCATCGAG
  GACATTCAGGAGGTGCGAATGGGGCACCGCACGGAGGGTCTGGAGAAGTTCGCCCGTGATGTGCCCGAGG
  ACCGCTGCTTCTCCATTGTCTTCAAGGACCAGCGCAATACACTAGACCTCATCGCCCCATCGCCAGCTGA
  TGCCCAGCACTGGGTGCTGGGGCTGCACAAGATCATCCACCACTCAGGCTCCATGGACCAGCGTCAGAAG
  CTACAGCACTGGATTCACTCCTGCTTGCGAAAAGCTGACAAAAACAAGGACAACAAGATGAGCTTCAAGG
  AGCTGCAGAACTTCCTGAAGGAGCTCAACATCCAGGTGGACGACAGCTATGCCCGGAAGATCTTCAGGGA
  GTGTGACCACTCCCAGACAGACTCCCTGGAGGACGAGGAGATTGAGGCCTTCTACAAGATGCTGACCCAG
  CGGGTGGAGATCGACCGCACCTTCGCCGAGGCCGCGGGCTCAGGGGAGACTCTGTCGGTGGATCAGTTAG
  TGACGTTCCTGCAGCACCAGCAGCGGGAGGAGGCGGCAGGGCCTGCGCTGGCCCTCTCCCTCATTGAGCG
  CTACGAGCCCAGCGAGACTGCCAAGGCGCAGCGGCAGATGACCAAGGACGGCTTCCTCATGTACTTACTG
  TCGGCTGACGGCAGCGCCTTCAGCCTGGCACACCGCCGTGTCTACCAGGACATGGGCCAGCCACTTAGCC
  ACTACCTGGTGTCCTCTTCACACAACACCTACCTGCTGGAGGACCAGCTAGCCGGGCCCAGCAGCACTGA
  AGCCTACATCCGGGCACTGTGCAAAGGCTGCCGATGCCTGGAGCTTGACTGCTGGGACGGGCCCAACCAG
  GAACCAATCATCTACCACGGCTATACTTTCACTTCCAAGATCCTCTTCTGCGATGTGCTCAGGGCCATCC
  GGGACTATGCCTTCAAGGCGTCCCCCTACCCTGTCATCCTATCCCTGGAGAACCACTGCACACTGGAGCA
  GCAGCGCGTGATGGCGCGGCACCTGCATGCCATCCTGGGCCCCATGCTGTTGAACCGACCACTGGATGGG
  GTCACCAACAGCCTGCCCTCCCCTGAGCAACTGAAGGGGAAGATCCTGCTGAAGGGGAAGAAGCTCGGGG
  GGCTCCTGCCCCCTGGAGGGGAGGGTGGCCCTGAGGCCACTGTGGTGTCAGACGAAGACGAGGCTGCTGA
  GATGGAGGATGAGGCAGTGAGGAGCCGTGTGCAGCACAAGCCCAAGGAGGACAAGCTCAGGCTAGCACAG
  GAGCTCTCTGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGGGGCTTCTCCAGTCCTGGCACCCCTG
  GACAGGCCTTCTACGAGATGGCGTCCTTCTCTGAGAACCGTGCCCTTCGACTGCTCCAAGAATCAGGAAA
  CGGCTTTGTCCGCCACAACGTGGGGCACCTGAGCAGAATCTACCCGGCTGGATGGAGAACAGACTCCTCC
  AACTACAGCCCCGTGGAGATGTGGAATGGGGGCTGCCAGATCGTGGCCCTGAATTTCCAGACACCTGGGC
  CAGAGATGGACGTGTACCAGGGCCGCTTCCAGGACAACGGGGCCTGTGGGTACGTGCTGAAGCCCGCCTT
  CCTGCGAGACCCCAACGGCACCTTTAACCCCCGCGCCCTGGCTCAGGGGCCCTGGTGGGCACGGAAGCGG
  CTCAACATCAGGGTCATTTCGGGGCAGCAGCTGCCAAAAGTCAACAAGAATAAGAATTCAATTGTGGACC
  CCAAAGTGACAGTGGAGATCCATGGCGTGAGCCGGGACGTGGCCAGCCGCCAGACTGCTGTCATCACCAA
  CAATGGTTTCAACCCATGGTGGGACACGGAGTTTGCGTTTGAGGTAGTTGTGCCTGACCTTGCCCTCATC
  CGCTTCTTGGTGGAAGATTATGATGCCTCCTCCAAGAATGACTTCATTGGCCAGAGTACCATCCCCTTGA
  ACAGCCTCAAGCAAGGATACCGCCATGTCCACCTCATGTCTAAGAACGGGGACCAGCATCCATCAGCCAC
  CCTCTTTGTGAAGATCTCCCTCCAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208263 representing NM_006225
  Red=Cloning site Green=Tags(s)
  
  MDSGRDFLTLHGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRTPESQLFSIE
  DIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMDQRQK
  LQHWIHSCLRKADKNKDNKMSFKELQNFLKELNIQVDDSYARKIFRECDHSQTDSLEDEEIEAFYKMLTQ
  RVEIDRTFAEAAGSGETLSVDQLVTFLQHQQREEAAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLL
  SADGSAFSLAHRRVYQDMGQPLSHYLVSSSHNTYLLEDQLAGPSSTEAYIRALCKGCRCLELDCWDGPNQ
  EPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCTLEQQRVMARHLHAILGPMLLNRPLDG
  VTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEMEDEAVRSRVQHKPKEDKLRLAQ
  ELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGFVRHNVGHLSRIYPAGWRTDSS
  NYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLRDPNGTFNPRALAQGPWWARKR
  LNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNGFNPWWDTEFAFEVVVPDLALI
  RFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLFVKISLQD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006225
• ORF Size: 2268 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006225.2, NP_006216.2
• RefSeq Size: 2707 bp
• RefSeq ORF: 2271 bp
• Locus ID: 5333
• Cytogenetics: 3p22.2
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: 'This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]'