PLCD1 (NM_006225) Human Untagged Clone

CAT#: SC303752

PLCD1 (untagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLCD1
• Synonyms: NDNC3; PLC-III
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006225 edited
  CCGCACTCCGCTCGGACCCCAGGCCGCCGGTGCTGTCGCTACTCAAGTGAGTCCCGCGGT
  GCCCCTCCCGCCGCGCCGCCCGTCGGGCATGGACTCGGGCCGGGACTTCCTGACCCTGCA
  CGGCCTACAGGATGATGAGGATCTACAGGCGCTGCTGAAGGGCAGCCAGCTCCTGAAGGT
  GAAGTCCAGCTCATGGAGGAGAGAGCGCTTCTACAAGTTGCAGGAGGACTGCAAGACCAT
  CTGGCAGGAGTCCCGCAAGGTCATGCGGACCCCGGAGTCCCAGCTGTTCTCCATCGAGGA
  CATTCAGGAGGTGCGAATGGGGCACCGCACGGAGGGTCTGGAGAAGTTCGCCCGTGATGT
  GCCCGAGGACCGCTGCTTCTCCATTGTCTTCAAGGACCAGCGCAATACACTAGACCTCAT
  CGCCCCATCGCCAGCTGATGCCCAGCACTGGGTGCTGGGGCTGCACAAGATCATCCACCA
  CTCAGGCTCCATGGACCAGCGTCAGAAGCTACAGCACTGGATTCACTCCTGCTTGCGAAA
  AGCTGACAAAAACAAGGACAACAAGATGAGCTTCAAGGAGCTGCAGAACTTCCTGAAGGA
  GCTCAACATCCAGGTGGACGACAGCTATGCCCGGAAGATCTTCAGGGAGTGTGACCACTC
  CCAGACAGACTCCCTGGAGGACGAGGAGATTGAGGCCTTCTACAAGATGCTGACCCAGCG
  GGTGGAGATCGACCGCACCTTCGCCGAGGCCGCGGGCTCAGGGGAGACTCTGTCGGTGGA
  TCAGTTAGTGACGTTCCTGCAGCACCAGCAGCGGGAGGAGGCGGCAGGGCCTGCGCTGGC
  CCTCTCCCTCATTGAGCGCTACGAGCCCAGCGAGACTGCCAAGGCGCAGCGGCAGATGAC
  CAAGGACGGCTTCCTCATGTACTTACTGTCGGCTGACGGCAGCGCCTTCAGCCTGGCACA
  CCGCCGTGTCTACCAGGACATGGGCCAGCCACTTAGCCACTACCTGGTGTCCTCTTCACA
  CAACACCTACCTGCTGGAGGACCAGCTAGCCGGGCCCAGCAGCACTGAAGCCTACATCCG
  GGCACTGTGCAAAGGCTGCCGATGCCTGGAGCTTGACTGCTGGGACGGGCCCAACCAGGA
  ACCAATCATCTACCACGGCTATACTTTCACTTCCAAGATCCTCTTCTGCGATGTGCTCAG
  GGCCATCCGGGACTATGCCTTCAAGGCGTCCCCCTACCCTGTCATCCTATCCCTGGAGAA
  CCACTGCACACTGGAGCAGCAGCGCGTGATGGCGCGGCACCTGCATGCCATCCTGGGCCC
  CATGCTGTTGAACCGACCACTGGATGGGGTCACCAACAGCCTGCCCTCCCCTGAGCAACT
  GAAGGGGAAGATCCTGCTGAAGGGGAAGAAGCTCGGGGGGCTCCTGCCCCCTGGAGGGGA
  GGGTGGCCCTGAGGCCACTGTGGTGTCAGACGAAGACGAGGCTGCTGAGATGGAGGATGA
  GGCAGTGAGGAGCCGTGTGCAGCACAAGCCCAAGGAGGACAAGCTCAGGCTAGCACAGGA
  GCTCTCTGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGGGGCTTCTCCAGTCCTGG
  CACCCCTGGACAGGCCTTCTACGAGATGGCGTCCTTCTCTGAGAACCGTGCCCTTCGACT
  GCTCCAAGAATCAGGAAACGGCTTTGTCCGCCACAACGTGGGGCACCTGAGCAGAATCTA
  CCCGGCTGGATGGAGAACAGACTCCTCCAACTACAGCCCCGTGGAGATGTGGAATGGGGG
  CTGCCAGATCGTGGCCCTGAATTTCCAGACACCTGGGCCAGAGATGGACGTGTACCAGGG
  CCGCTTCCAGGACAACGGGGCCTGTGGGTACGTGCTGAAGCCCGCCTTCCTGCGAGACCC
  CAACGGCACCTTTAACCCCCGCGCCCTGGCTCAGGGGCCCTGGTGGGCACGGAAGCGGCT
  CAACATCAGGGTCATTTCGGGGCAGCAGCTGCCAAAAGTCAACAAGAATAAGAATTCAAT
  TGTGGACCCCAAAGTGACAGTGGAGATCCATGGCGTGAGCCGGGACGTGGCCAGCCGCCA
  GACTGCTGTCATCACCAACAATGGTTTCAACCCATGGTGGGACACGGAGTTTGCGTTTGA
  GGTAGTTGTGCCTGACCTTGCCCTCATCCGCTTCTTGGTGGAAGATTATGATGCCTCCTC
  CAAGAATGACTTCATTGGCCAGAGTACCATCCCCTTGAACAGCCTCAAGCAAGGATACCG
  CCATGTCCACCTCATGTCTAAGAACGGGGACCAGCATCCATCAGCCACCCTCTTTGTGAA
  GATCTCCCTCCAGGACTAGGCTGGAGGAAGCCAGTGGGGTCCCCCCTGAGTGGGCTGGGC
  CCTCTGTCCACATGTGGGGACAGGGCTGGTGTGGCTGCTCCCAGCCTCTTGCTCAGAGCT
  AGGCCCCCAAATTGCCTTCAGCCCTAACATAGTGTCTGCTGCTGCCTCCCTGGGGACCAG
  GAGCTAGCCCAGTCCCTGGAGCTGTCCTTCATTCCGTTAGGAATAACACTGCAGCCCTCT
  CCACCCTCCGGCCAGCGAGTGGTCAAGGATTTTTATAAAAATCACGATAAGATTAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006225
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006225.1, NP_006216.1
• RefSeq Size: 2627 bp
• RefSeq ORF: 2271 bp
• Locus ID: 5333
• Cytogenetics: 3p22.2
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: 'This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to transcript variant 1, and initiates translation from an in-frame upstream AUG, resulting in a shorter isoform (2) with a different N-terminus compared to isoform 1.