PSMA2 (NM_002787) Human Tagged ORF Clone

CAT#: RG208272

PSMA2 (GFP-tagged) - Human proteasome (prosome, macropain) subunit, alpha type, 2 (PSMA2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PSMA2
• Synonyms: HC3; MU; PMSA2; PSC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208272 representing NM_002787
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCGCGGGTACAGCTTTTCGCTGACTACATTCAGCCCGTCTGGTAAACTTGTCCAGATTGAAT
  ATGCTTTGGCTGCTGTAGCTGGAGGAGCCCCGTCCGTGGGAATTAAAGCTGCAAATGGTGTGGTATTAGC
  AACTGAGAAAAAACAGAAATCCATTCTGTATGATGAGCGAAGTGTACACAAAGTAGAACCAATTACCAAG
  CATATAGGTTTGGTGTACAGTGGCATGGGCCCCGATTACAGAGTGCTTGTGCACAGAGCTCGAAAACTAG
  CTCAACAATACTATCTTGTGTACCAAGAACCCATTCCTACAGCTCAGCTGGTACAGAGAGTAGCTTCTGT
  GATGCAAGAATATACTCAGTCAGGTGGTGTTCGTCCATTTGGAGTTTCTTTACTTATTTGTGGTTGGAAT
  GAGGGACGACCATATTTATTTCAGTCAGATCCATCTGGAGCTTACTTTGCCTGGAAAGCTACAGCAATGG
  GAAAGAACTATGTGAATGGGAAGACTTTCCTTGAGAAAAGATATAATGAAGATCTGGAACTTGAAGATGC
  CATTCATACAGCCATCTTAACCCTAAAGGAAAGCTTTGAAGGGCAAATGACAGAGGATAACATAGAAGTT
  GGAATCTGCAATGAAGCTGGATTTAGGAGGCTTACTCCAACTGAAGTTAAGGATTACTTGGCTGCCATAG
  CA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208272 representing NM_002787
  Red=Cloning site Green=Tags(s)
  
  MAERGYSFSLTTFSPSGKLVQIEYALAAVAGGAPSVGIKAANGVVLATEKKQKSILYDERSVHKVEPITK
  HIGLVYSGMGPDYRVLVHRARKLAQQYYLVYQEPIPTAQLVQRVASVMQEYTQSGGVRPFGVSLLICGWN
  EGRPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNEDLELEDAIHTAILTLKESFEGQMTEDNIEV
  GICNEAGFRRLTPTEVKDYLAAIA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002787
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002787.3, NP_002778.1
• RefSeq Size: 1477 bp
• RefSeq ORF: 705 bp
• Locus ID: 5683
• Cytogenetics: 7p14.1
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq, Jul 2008]'