PIGZ (NM_025163) Human Tagged ORF Clone

CAT#: RG208313

PIGZ (GFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIGZ
• Synonyms: GPI-MT-IV; PIG-Z; SMP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208313 representing NM_025163
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATCTGTGGATCCAGCGTAGCATCTGTAGCAGCTGGGACATCATTCCAGGTTTTGGGCCCGGTGT
  GTTGGCAACAACTGGATCTGAAGATGGCAGTCAGGGTGCTTTGGGGTGGTCTCAGCCTGCTCCGAGTGCT
  GTGGTGTCTCCTTCCGCAGACGGGCTATGTGCACCCAGATGAGTTCTTCCAGTCCCCTGAGGTGATGGCA
  GAGGACATCCTGGGCGTTCAGGCCGCGCGGCCCTGGGAGTTTTACCCCAGCAGCTCCTGCCGCTCGGTGC
  TCTTCCCCCTGCTGATCTCCGGTTCCACCTTCTGGCTGCTCAGGCTCTGGGAGGAGCTGGGGCCGTGGCC
  TGGCCTGGTGAGCGGCTATGCGCTGCTGGTGGGGCCTCGACTCCTCCTCACTGCCCTTTCCTTTGCTCTG
  GACGGGGCCGTGTACCACCTGGCCCCGCCGATGGGGGCGGATCGCTGGAACGCCCTGGCCCTGCTGTCTG
  GTTCCTACGTCACCCTGGTCTTCTACACAAGGACCTTCTCCAACACCATTGAGGGACTCCTCTTCACGTG
  GCTGCTGGTGCTGGTATCCTCCCATGTAACGTGGGGCCCTACACGCAAGGAGCCGGCGCCGGGTCCACGG
  TGGCGCAGCTGGCTTCTTGGAGGCATTGTGGCTGCTGGCTTCTTCAACCGGCCCACCTTTCTGGCCTTTG
  CTGTGGTCCCCCTCTACCTCTGGGGCACTCGTGGAGCCACAAACCCTGGTTTGAAGTCTCTGACCCGGGA
  GGCCCTGGTGCTGCTCCCTGGGGCGACCCTCACAGCAGCGGTGTTTGTGGCCACGGACAGCTGGTATTTC
  TCCAGCCCCGCTACATCCAGGAACCTTGTCCTGACACCTGTCAACTTCCTGCACTACAACCTGAATCCCC
  AAAACCTGGCGAGACATGGCACGCACGCGCGGCTCACTCACCTGGCAGTCAACGGCTTCCTGCTCTTCGG
  GGTGCTGCATGCCCAGGCCCTGCAGGCTGCGTGGCAACAGCTGCAAGTCGGCCTCCAGGCCTCTGCACAA
  ATGGGCCTCCTGAGGGCACTGGGTGCCCGGAGCCTGCTGTCCAGCCCCAGGTCCTATCTCCTTCTCCTCT
  ACTTCATGCCTCTGGCCCTGCTATCTGCCTTTAGCCACCAGGAGGCTCGGTTCCTGATTCCCCTCCTGGT
  CCCCCTGGTCCTGCTTTGTAGTCCACAGACGCAGCCTGTGCCTTGGAAGGGCACTGTGGTCCTCTTCAAC
  GCCCTCGGTGCCCTCCTCTTCGGCTGCCTGCATCAGGGGGGCCTGGTGCCTGGCCTGGAGTACCTGGAGC
  AGGTGGTCCATGCCCCTGTGCTCCCAAGCACACCCACCCACTACACACTCCTCTTCACTCACACCTACAT
  GCCCCCCCGGCACCTCCTACACCTCCCAGGCCTGGGGGCACCAGTGGAGGTGGTGGACATAGGGGGGACT
  GAGGACTGGGCCCTGTGCCAAACCCTGAAAAGCTTCACCAGACAACCAGCCTGCCAAGTGGCTGGTGGGC
  CATGGCTCTGCCGCCTCTTTGTGGTAACCCCTGGCACCACCAGGCGTGCCGTGGAGAAGTGCAGCTTCCC
  CTTCAAGAATGAAACACTTTTATTTCCCCATCTGACCCTGGAGGATCCACCAGCCCTGTCCTCCTTGCTG
  AGTGGGGCTTGGAGGGACCACCTCAGTCTTCACATTGTGGAGCTGGGGGAAGAAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208313 representing NM_025163
  Red=Cloning site Green=Tags(s)
  
  MQICGSSVASVAAGTSFQVLGPVCWQQLDLKMAVRVLWGGLSLLRVLWCLLPQTGYVHPDEFFQSPEVMA
  EDILGVQAARPWEFYPSSSCRSVLFPLLISGSTFWLLRLWEELGPWPGLVSGYALLVGPRLLLTALSFAL
  DGAVYHLAPPMGADRWNALALLSGSYVTLVFYTRTFSNTIEGLLFTWLLVLVSSHVTWGPTRKEPAPGPR
  WRSWLLGGIVAAGFFNRPTFLAFAVVPLYLWGTRGATNPGLKSLTREALVLLPGATLTAAVFVATDSWYF
  SSPATSRNLVLTPVNFLHYNLNPQNLARHGTHARLTHLAVNGFLLFGVLHAQALQAAWQQLQVGLQASAQ
  MGLLRALGARSLLSSPRSYLLLLYFMPLALLSAFSHQEARFLIPLLVPLVLLCSPQTQPVPWKGTVVLFN
  ALGALLFGCLHQGGLVPGLEYLEQVVHAPVLPSTPTHYTLLFTHTYMPPRHLLHLPGLGAPVEVVDIGGT
  EDWALCQTLKSFTRQPACQVAGGPWLCRLFVVTPGTTRRAVEKCSFPFKNETLLFPHLTLEDPPALSSLL
  SGAWRDHLSLHIVELGEET
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025163
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025163.2, NP_079439.2
• RefSeq Size: 2721
• RefSeq ORF: 1740
• Locus ID: 80235
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Gene Summary: The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq, Jul 2008]