LAT2 (SLC7A8) (NM_012244) Human Tagged ORF Clone

CAT#: RG208586

SLC7A8 (GFP-tagged) - Human solute carrier family 7 (amino acid transporter, L-type), member 8 (SLC7A8), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC7A8
• Synonyms: LAT2; LPI-PC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208586 representing NM_012244
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAGGAGCCAGGCACCGAAACAACACCGAAAAGAAACACCCAGGTGGGGGCGAGTCGGACGCCA
  GCCCCGAGGCTGGTTCCGGAGGGGGCGGAGTAGCCCTGAAGAAAGAGATCGGATTGGTCAGTGCCTGTGG
  TATCATCGTAGGGAACATCATCGGCTCTGGAATCTTTGTCTCGCCAAAGGGAGTGCTGGAGAATGCTGGT
  TCTGTGGGCCTTGCTCTCATCGTCTGGATTGTGACGGGCTTCATCACAGTTGTGGGAGCCCTCTGCTATG
  CTGAACTCGGGGTCACCATCCCCAAATCTGGAGGTGACTACTCCTATGTCAAGGACATCTTCGGAGGACT
  GGCTGGGTTCCTGAGGCTGTGGATTGCTGTGCTGGTGATCTACCCCACCAACCAGGCTGTCATCGCCCTC
  ACCTTCTCCAACTACGTGCTGCAGCCGCTCTTCCCCACCTGCTTCCCCCCAGAGTCTGGCCTTCGGCTCC
  TGGCTGCCATCTGCTTATTGCTCCTCACATGGGTCAACTGTTCCAGTGTGCGGTGGGCCACCCGGGTTCA
  AGACATCTTCACAGCTGGGAAGCTCCTGGCCTTGGCCCTGATTATCATCATGGGGATTGTACAGATATGC
  AAAGGAGAGTACTTCTGGCTGGAGCCAAAGAATGCATTTGAGAATTTCCAGGAACCTGACATCGGCCTCG
  TCGCACTGGCTTTCCTTCAGGGCTCCTTTGCCTATGGAGGCTGGAACTTTCTGAATTACGTGACTGAGGA
  GCTTGTTGATCCCTACAAGAACCTTCCCAGAGCCATCTTCATCTCCATCCCACTGGTCACATTTGTGTAT
  GTCTTTGCCAATGTCGCTTATGTCACTGCAATGTCCCCCCAGGAGCTGCTGGCATCCAACGCCGTCGCTG
  TGACTTTTGGAGAGAAGCTCCTAGGAGTCATGGCCTGGATCATGCCCATTTCTGTTGCCCTGTCCACATT
  TGGAGGAGTTAATGGGTCTCTCTTCACCTCCTCTCGGCTGTTCTTCGCTGGAGCCCGAGAGGGCCACCTT
  CCCAGTGTGTTGGCCATGATCCACGTGAAGCGCTGCACCCCAATCCCAGCCCTGCTCTTCACATGCATCT
  CCACCCTGCTGATGCTGGTCACCAGTGACATGTACACACTCATCAACTACGTGGGCTTCATCAACTACCT
  CTTCTATGGGGTCACGGTTGCTGGACAGATAGTCCTTCGCTGGAAGAAGCCTGATATCCCCCGCCCCATC
  AAGATCAACCTGCTGTTCCCCATCATCTACTTGCTGTTCTGGGCCTTCCTGCTGGTCTTCAGCCTGTGGT
  CAGAGCCGGTGGTGTGTGGCATTGGCCTGGCCATCATGCTGACAGGAGTGCCTGTCTATTTCCTGGGTGT
  TTACTGGCAACACAAGCCCAAGTGTTTCAGTGACTTCATTGAGCTGCTAACCCTGGTGAGCCAGAAGATG
  TGTGTGGTCGTGTACCCCGAGGTGGAGCGGGGCTCAGGGACAGAGGAGGCTAATGAGGACATGGAGGAGC
  AGCAGCAGCCCATGTACCAACCCACTCCCACGAAGGACAAGGACGTGGCGGGGCAGCCCCAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208586 representing NM_012244
  Red=Cloning site Green=Tags(s)
  
  MEEGARHRNNTEKKHPGGGESDASPEAGSGGGGVALKKEIGLVSACGIIVGNIIGSGIFVSPKGVLENAG
  SVGLALIVWIVTGFITVVGALCYAELGVTIPKSGGDYSYVKDIFGGLAGFLRLWIAVLVIYPTNQAVIAL
  TFSNYVLQPLFPTCFPPESGLRLLAAICLLLLTWVNCSSVRWATRVQDIFTAGKLLALALIIIMGIVQIC
  KGEYFWLEPKNAFENFQEPDIGLVALAFLQGSFAYGGWNFLNYVTEELVDPYKNLPRAIFISIPLVTFVY
  VFANVAYVTAMSPQELLASNAVAVTFGEKLLGVMAWIMPISVALSTFGGVNGSLFTSSRLFFAGAREGHL
  PSVLAMIHVKRCTPIPALLFTCISTLLMLVTSDMYTLINYVGFINYLFYGVTVAGQIVLRWKKPDIPRPI
  KINLLFPIIYLLFWAFLLVFSLWSEPVVCGIGLAIMLTGVPVYFLGVYWQHKPKCFSDFIELLTLVSQKM
  CVVVYPEVERGSGTEEANEDMEEQQQPMYQPTPTKDKDVAGQPQP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012244
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012244.2, NP_036376.2
• RefSeq Size: 4237
• RefSeq ORF: 1608
• Locus ID: 23428
• Domains: aa_permeases
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. [UniProtKB/Swiss-Prot Function]