GPC1 (NM_002081) Human Tagged ORF Clone

CAT#: RG208602

GPC1 (GFP-tagged) - Human glypican 1 (GPC1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPC1
• Synonyms: glypican
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208602 representing NM_002081
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCGGGCCCGAGGCTGGTGGCTGCTATGTGCGGCCGCAGCGCTGGTCGCCTGCGCCCGCGGGG
  ACCCGGCCAGCAAGAGCCGGAGCTGCGGCGAGGTCCGCCAGATCTACGGAGCCAAGGGCTTCAGCCTGAG
  CGACGTGCCCCAGGCGGAGATCTCGGGTGAGCACCTGCGGATCTGTCCCCAGGGCTACACCTGCTGCACC
  AGCGAGATGGAGGAGAACCTGGCCAACCGCAGCCATGCCGAGCTGGAGACCGCGCTCCGGGACAGCAGCC
  GCGTCCTGCAGGCCATGCTTGCCACCCAGCTGCGCAGCTTCGATGACCACTTCCAGCACCTGCTGAACGA
  CTCGGAGCGGACGCTGCAGGCCACCTTCCCCGGCGCCTTCGGAGAGCTGTACACGCAGAACGCGAGGGCC
  TTCCGGGACCTGTACTCAGAGCTGCGCCTGTACTACCGCGGTGCCAACCTGCACCTGGAGGAGACGCTGG
  CCGAGTTCTGGGCCCGCCTGCTCGAGCGCCTCTTCAAGCAGCTGCACCCCCAGCTGCTGCTGCCTGATGA
  CTACCTGGACTGCCTGGGCAAGCAGGCCGAGGCGCTGCGGCCCTTCGGGGAGGCCCCGAGAGAGCTGCGC
  CTGCGGGCCACCCGTGCCTTCGTGGCTGCTCGCTCCTTTGTGCAGGGCCTGGGCGTGGCCAGCGACGTGG
  TCCGGAAAGTGGCTCAGGTCCCCCTGGGCCCGGAGTGCTCGAGAGCTGTCATGAAGCTGGTCTACTGTGC
  TCACTGCCTGGGAGTCCCCGGCGCCAGGCCCTGCCCTGACTATTGCCGAAATGTGCTCAAGGGCTGCCTT
  GCCAACCAGGCCGACCTGGACGCCGAGTGGAGGAACCTCCTGGACTCCATGGTGCTCATCACCGACAAGT
  TCTGGGGTACATCGGGTGTGGAGAGTGTCATCGGCAGCGTGCACACGTGGCTGGCGGAGGCCATCAACGC
  CCTCCAGGACAACAGGGACACGCTCACGGCCAAGGTCATCCAGGGCTGCGGGAACCCCAAGGTCAACCCC
  CAGGGCCCTGGGCCTGAGGAGAAGCGGCGCCGGGGCAAGCTGGCCCCGCGGGAGAGGCCACCTTCAGGCA
  CGCTGGAGAAGCTGGTCTCTGAAGCCAAGGCCCAGCTCCGCGACGTCCAGGACTTCTGGATCAGCCTCCC
  AGGGACACTGTGCAGTGAGAAGATGGCCCTGAGCACTGCCAGTGATGACCGCTGCTGGAACGGGATGGCC
  AGAGGCCGGTACCTCCCCGAGGTCATGGGTGACGGCCTGGCCAACCAGATCAACAACCCCGAGGTGGAGG
  TGGACATCACCAAGCCGGACATGACCATCCGGCAGCAGATCATGCAGCTGAAGATCATGACCAACCGGCT
  GCGCAGCGCCTACAACGGCAACGACGTGGACTTCCAGGACGCCAGTGACGACGGCAGCGGCTCGGGCAGC
  GGTGATGGCTGTCTGGATGACCTCTGCGGCCGGAAGGTCAGCAGGAAGAGCTCCAGCTCCCGGACGCCCT
  TGACCCATGCCCTCCCAGGCCTGTCAGAGCAGGAAGGACAGAAGACCTCGGCTGCCAGCTGCCCCCAGCC
  CCCGACCTTCCTCCTGCCCCTCCTCCTCTTCCTGGCCCTTACAGTAGCCAGGCCCCGGTGGCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208602 representing NM_002081
  Red=Cloning site Green=Tags(s)
  
  MELRARGWWLLCAAAALVACARGDPASKSRSCGEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCT
  SEMEENLANRSHAELETALRDSSRVLQAMLATQLRSFDDHFQHLLNDSERTLQATFPGAFGELYTQNARA
  FRDLYSELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLLPDDYLDCLGKQAEALRPFGEAPRELR
  LRATRAFVAARSFVQGLGVASDVVRKVAQVPLGPECSRAVMKLVYCAHCLGVPGARPCPDYCRNVLKGCL
  ANQADLDAEWRNLLDSMVLITDKFWGTSGVESVIGSVHTWLAEAINALQDNRDTLTAKVIQGCGNPKVNP
  QGPGPEEKRRRGKLAPRERPPSGTLEKLVSEAKAQLRDVQDFWISLPGTLCSEKMALSTASDDRCWNGMA
  RGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRSAYNGNDVDFQDASDDGSGSGS
  GDGCLDDLCGRKVSRKSSSSRTPLTHALPGLSEQEGQKTSAASCPQPPTFLLPLLLFLALTVARPRWR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002081
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002081.1, NP_002072.1
• RefSeq Size: 3692 bp
• RefSeq ORF: 1677 bp
• Locus ID: 2817
• Cytogenetics: 2q37.3
• Domains: Glypican
• Protein Families: Druggable Genome
• Gene Summary: 'Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]'