GPC1 (NM_002081) Human Untagged Clone

CAT#: SC321494

GPC1 (untagged)-Human glypican 1 (GPC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPC1
• Synonyms: glypican
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002081.1 GGCTGCCCGAGCGAGCGTTCGGACCTCGCACCCCGCGCGCCCCGCGCCGCCGCCGCCGCC
  GGCTTTTGTTGTCTCCGCCTCCTCGGCCGCCGCCGCCTCTGGACCGCGAGCCGCGCGCGC
  CGGGACCTTGGCTCTGCCCTTCGCGGGCGGGAACTGCGCAGGACCCGGCCAGGATCCGAG
  AGAGGCGCGGGCGGGTGGCCGGGGGCGCCGCCGGCCCCGCCATGGAGCTCCGGGCCCGAG
  GCTGGTGGCTGCTATGTGCGGCCGCAGCGCTGGTCGCCTGCGCCCGCGGGGACCCGGCCA
  GCAAGAGCCGGAGCTGCGGCGAGGTCCGCCAGATCTACGGAGCCAAGGGCTTCAGCCTGA
  GCGACGTGCCCCAGGCGGAGATCTCGGGTGAGCACCTGCGGATCTGTCCCCAGGGCTACA
  CCTGCTGCACCAGCGAGATGGAGGAGAACCTGGCCAACCGCAGCCATGCCGAGCTGGAGA
  CCGCGCTCCGGGACAGCAGCCGCGTCCTGCAGGCCATGCTTGCCACCCAGCTGCGCAGCT
  TCGATGACCACTTCCAGCACCTGCTGAACGACTCGGAGCGGACGCTGCAGGCCACCTTCC
  CCGGCGCCTTCGGAGAGCTGTACACGCAGAACGCGAGGGCCTTCCGGGACCTGTACTCAG
  AGCTGCGCCTGTACTACCGCGGTGCCAACCTGCACCTGGAGGAGACGCTGGCCGAGTTCT
  GGGCCCGCCTGCTCGAGCGCCTCTTCAAGCAGCTGCACCCCCAGCTGCTGCTGCCTGATG
  ACTACCTGGACTGCCTGGGCAAGCAGGCCGAGGCGCTGCGGCCCTTCGGGGAGGCCCCGA
  GAGAGCTGCGCCTGCGGGCCACCCGTGCCTTCGTGGCTGCTCGCTCCTTTGTGCAGGGCC
  TGGGCGTGGCCAGCGACGTGGTCCGGAAAGTGGCTCAGGTCCCCCTGGGCCCGGAGTGCT
  CGAGAGCTGTCATGAAGCTGGTCTACTGTGCTCACTGCCTGGGAGTCCCCGGCGCCAGGC
  CCTGCCCTGACTATTGCCGAAATGTGCTCAAGGGCTGCCTTGCCAACCAGGCCGACCTGG
  ACGCCGAGTGGAGGAACCTCCTGGACTCCATGGTGCTCATCACCGACAAGTTCTGGGGTA
  CATCGGGTGTGGAGAGTGTCATCGGCAGCGTGCACACGTGGCTGGCGGAGGCCATCAACG
  CCCTCCAGGACAACAGGGACACGCTCACGGCCAAGGTCATCCAGGGCTGCGGGAACCCCA
  AGGTCAACCCCCAGGGCCCTGGGCCTGAGGAGAAGCGGCGCCGGGGCAAGCTGGCCCCGC
  GGGAGAGGCCACCTTCAGGCACGCTGGAGAAGCTGGTCTCTGAAGCCAAGGCCCAGCTCC
  GCGACGTCCAGGACTTCTGGATCAGCCTCCCAGGGACACTGTGCAGTGAGAAGATGGCCC
  TGAGCACTGCCAGTGATGACCGCTGCTGGAACGGGATGGCCAGAGGCCGGTACCTCCCCG
  AGGTCATGGGTGACGGCCTGGCCAACCAGATCAACAACCCCGAGGTGGAGGTGGACATCA
  CCAAGCCGGACATGACCATCCGGCAGCAGATCATGCAGCTGAAGATCATGACCAACCGGC
  TGCGCAGCGCCTACAACGGCAACGACGTGGACTTCCAGGACGCCAGTGACGACGGCAGCG
  GCTCGGGCAGCGGTGATGGCTGTCTGGATGACCTCTGCGGCCGGAAGGTCAGCAGGAAGA
  GCTCCAGCTCCCGGACGCCCTTGACCCATGCCCTCCCAGGCCTGTCAGAGCAGGAAGGAC
  AGAAGACCTCGGCTGCCAGCTGCCCCCAGCCCCCGACCTTCCTCCTGCCCCTCCTCCTCT
  TCCTGGCCCTTACAGTAGCCAGGCCCCGGTGGCGGTAACTGCCCCAAGGCCCCAGGGACA
  GAGGCCAAGGACTGACTTTGCCAAAAATACAACACAGACGATATTTAATTCAAAAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002081
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002081.1, NP_002072.1
• RefSeq Size: 3692 bp
• RefSeq ORF: 1677 bp
• Locus ID: 2817
• Cytogenetics: 2q37.3
• Domains: Glypican
• Protein Families: Druggable Genome
• Gene Summary: 'Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]'